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SwaI
SwaI
| CAT.NO | UNIT |
|---|---|
| E061 | 10000 U (500 µl) |
| SKU | E061 |
|---|---|
| Unit quantity | 10000 U (500 µl) |
| Applications | Restriction enzyme digestion of double-stranded DNA |
| Components | SwaI enzyme supplied with 10X Restriction Enzyme Reaction Buffer 3 (Cat.No. E203) |
| Concentration | 20000 U/ml |
| Expression System | An E. coli strain that carries the SwaI gene from Staphylococcus warneri . The SwaI enzyme recognizes and cuts the sequence ATTT^AAAT. |
| Inactivation Protocol | 65°C for 20 min |
| Reaction Definition | 1X Restriction Enzyme Reaction Buffer 3 (Cat.No. E203). Incubate at 25°C. SwaI is unstable at 37°C and has reduced activity if incubated at 37°C. |
| Reaction Buffer | Buffer 1: 10%, Buffer 2: 10%, Buffer 3: 100%, Universal Buffer: 10% Click here to view the buffer compatibility chart for restriction enzymes. |
| Storage Condition | -20°C |
| Storage Buffer | 10mM Tris pH (7.5), 400mM NaCl, 1mM DTT, 0.1mM EDTA, and 50% (v/v) Glycerol. |
| Note | Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Not Sensitive |
