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SacI
SacI
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| CAT.NO | UNIT |
|---|---|
| E073 | 10000 U (500 µl) |
| SKU | E073 |
|---|---|
| Unit quantity | 10000 U (500 µl) |
| Applications | Restriction enzyme digestion of double-stranded DNA |
| Components | SacI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204) |
| Concentration | 20000 U/ml |
| Reaction Definition | 1X Universal Restriction Enzyme Buffer (Cat.No. E204). Incubate at 37°C. |
| Reaction Buffer | Buffer 1: 10%, Buffer 2: 50%, Buffer 3: 10%, Universal Buffer: 100% Click here to view the buffer compatibility chart for restriction enzymes. |
| Storage Condition | -20°C |
| Storage Buffer | 0.1 mM EDTA, 50% Glycerol, 1 mM DTT, 10 mM Tris-HCl, 100 mM NaCl, 200 µg/ml BSA, pH 7.4 @ 25°C. |
| Note | Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Not Sensitive |
