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MspI
MspI
| CAT.NO | UNIT |
|---|---|
| E072 | 10000 U (500ul) |
| SKU | E072 |
|---|---|
| Unit quantity | 10000 U (500ul) |
| Applications | Restriction enzyme digestion of double-stranded DNA. |
| Components | MspI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204) |
| Concentration | 20000 U/ml |
| Expression System | An E. coli strain that carries the cloned and modified MspI gene from Moraxella species (ATCC 49670). The MspI enzyme recognizes and cuts the sequence C^CGG. |
| Inactivation Protocol | No |
| Reaction Definition | 1X Universal Restriction Enzyme Buffer (Cat.No. E204). Incubate at 37°C. |
| Reaction Buffer | Buffer 1: 75%, Buffer 2: 100%, Buffer 3: 50%, Universal Buffer: 100% Click here to view the buffer compatibility chart for restriction enzymes. |
| Storage Condition | -20°C |
| Storage Buffer | 0.1 mM EDTA, 50% Glycerol, 1 mM DTT, 10 mM Tris-HCl, 50 mM KCl, 200 µg/ml BSA, pH 7.4 @ 25°C. |
| Note | Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Not Sensitive |
