HindIII
CAT.NO | UNIT |
---|---|
E071 | 10000 U (500ul) |
SKU | E071 |
---|---|
Unit quantity | 10000 U (500ul) |
Applications | Restriction enzyme digestion of double-stranded DNA |
Components | HindIII enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204) |
Concentration | 20000 U/ml |
Expression System | An E. coli strain that carries the cloned and modified HindIII gene from Haemophilus influenzae Rd (ATCC 51907). The HindIII enzyme recognizes and cuts the sequence A^AGCTT. |
Inactivation Protocol | 80°C for 20 min |
Reaction Definition | 1X Universal Restriction Enzyme Buffer (Cat.No. E204). Incubate at 37°C. |
Reaction Buffer | Buffer 1: 10%, Buffer 2: 100%, Buffer 3: 10%, Universal Buffer: 100% Click here to view the buffer compatibility chart for restriction enzymes. |
Storage Condition | -20°C |
Storage Buffer | 0.1 mM EDTA, 50% Glycerol, 1 mM DTT, 10 mM Tris-HCl, 300 mM NaCl, 500 µg/ml BSA, pH 7.4 @ 25°C. |
Note | Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Not Sensitive |