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EcoRV
EcoRV
| CAT.NO | UNIT |
|---|---|
| E059 | 10000 U (500 µl) |
| SKU | E059 |
|---|---|
| Unit quantity | 10000 U (500 µl) |
| Applications | Restriction enzyme digestion of double-stranded DNA |
| Components | EcoRV enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204) |
| Concentration | 20000 U/ml |
| Expression System | An E.coli strain that carries the modified EcoRV gene from the plasmid J62 pLG74 (L.l. Glatman). The EcoRV enzyme recognizes and cuts the sequence GAT^ATC. |
| Inactivation Protocol | 65°C for 20 min |
| Reaction Definition | 1X Universal Restriction Enzyme Reaction Buffer (Cat. No. E204). Incubate at 37°C. |
| Reaction Buffer | Buffer 1: 25%, Buffer 2: 100%, Buffer 3: 100%, Universal Buffer: 100%. Click here to view the buffer compatibility chart for restriction enzymes. |
| Storage Condition | -20°C |
| Storage Buffer | 10mM Tris pH (7.5), 100mM NaCl, 1mM DTT, 0.1mM EDTA, and 50% (v/v) Glycerol. |
| Note | Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Blocked by Some Combinations of Overlapping |
