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EcoRI
EcoRI
| CAT.NO | UNIT |
|---|---|
| E048 | 20000 U (1 ml) |
| SKU | E048 |
|---|---|
| Unit quantity | 20000 U (1 ml) |
| Applications | Restriction enzyme digestion of double-stranded DNA |
| Components | EcoRI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204) |
| Concentration | 20000 U/ml |
| Expression System | An E. coli strain that carries the modified EcoRI gene from E. coli RY13. The EcoRI enzyme recognizes and cuts the sequence G^AATTC. |
| Inactivation Protocol | 65°C for 20 min |
| Reaction Definition | 1X Universal Restriction Enzyme Reaction Buffer (Cat. No. E204). Incubate at 37°C |
| Reaction Buffer | Buffer 1: 10%, Buffer 2: 100%, Buffer 3: 10%, Universal Buffer: 100% Click here to view the buffer compatibility chart for restriction enzymes. |
| Storage Condition | -20°C |
| Storage Buffer | 20 mM Tris-HCl (pH 7.5), 300 mM NaCl, 1 mM DTT, 0.1 mM EDTA, and 50% (v/v) Glycerol |
| Note | Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Blocked by Some Combinations of Overlapping |
