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BglII

CAT.NO UNIT
E069 5000 U (500ul)
SKU E069
Unit quantity 5000 U (500ul)
Applications Restriction enzyme digestion of double-stranded DNA
Components BglII enzyme supplied with 10X Restriction Enzyme Reaction Buffer 3 (Cat.No. E203)
Concentration 10000 U/ml
Expression System AnE. coli strain that carries the cloned and modified BglII gene from Bacillus globigii (ATCC 49760). The BglII enzyme recognizes and cuts the sequence A^GATCT.
Inactivation Protocol No
Reaction Definition 1X Restriction Enzyme Reaction Buffer 3 (Cat.No. E203). Incubate at 37°C.
Reaction Buffer Buffer 1: 10%, Buffer 2: 10%, Buffer 3: 10%, Universal Buffer: 100%
Click here to view the buffer compatibility chart for restriction enzymes.
Storage Condition -20°C
Note Methylation Sensitivity
dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Not Sensitive

Supporting Protocol

MSDS
QC

Other