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AflII
AflII
| CAT.NO | UNIT |
|---|---|
| E050 | 10000 U (500 µl) |
| SKU | E050 |
|---|---|
| Unit quantity | 10000 U (500 µl) |
| Applications | Restriction enzyme digestion of double-stranded DNA |
| Components | AflII enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204) |
| Concentration | 20000 U/ml |
| Expression System | An E. coli strain that carries the AflII gene from Anabaena flos-aquae (CCAP 1403/13f). The AflII enzyme recognizes and cuts the sequence C^TTAAG. |
| Inactivation Protocol | 65°C for 20 min |
| Reaction Definition | 1X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204) |
| Reaction Buffer | Buffer 1: 50%, Buffer 2: 100%, Buffer 3: 10%, Universal Buffer: 100% Click here to view the buffer compatibility chart for restriction enzymes. |
| Storage Condition | -20°C |
| Storage Buffer | 10 mM Tris-HCl (pH 7.5), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, and 50% (v/v) Glycerol |
| Note | Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Not Sensitive |
