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T5004 · Research Use Only

Human Cardiomyocytes

Stop Waiting. Start Beating.

Ready-to-use human embryonic stem cell-derived cardiomyocytes are cryopreserved and functionally validated, with spontaneous contractions typically beginning within 3–5 days after plating.

T5004 provides a reproducible human cardiac model for drug discovery, cardiotoxicity screening, disease modelling, electrophysiology, and advanced 3D cardiac workflows.

Human cardiomyocytes
Functional recovery

Watch Human Cardiomyocytes Beat

Representative cultures demonstrate spontaneous contractions after plating, with beating typically observed within 3–5 days.

Functional validation

Ready-to-use cardiac cells for time-sensitive workflows

Genetic Stability

Embryonic stem cell origin supports consistent cellular identity.

Rapid Recovery

Spontaneous beating typically begins within 3–5 days.

Cardiac Identity

Key contractility markers are characterized by molecular and imaging assays.

Consistency

Controlled differentiation supports repeatable experimental outcomes.

Performance

Functional cardiac behaviour is maintained for roughly 10–14 days in culture.

Applications

Human-relevant cardiac workflows

Drug Discovery

Evaluate efficacy and cardiac responses during preclinical development.

Cardiotoxicity Screening

Identify adverse cardiac effects earlier during compound evaluation.

Disease Modelling

Study cardiac physiology and disease mechanisms in a human cell model.

Electrophysiology & Contractility

Assess beating behaviour, electrical activity, and functional response.

Tissue Engineering & Organoids

Build advanced 3D cardiac models and organoid systems.

Validation Data

Verified cardiac identity and function

T5004 undergoes molecular, phenotypic, and functional characterization to support cardiac lineage identity and performance.

RT-qPCR cardiomyocyte validation
RT-PCR Validation · Expression Analysis
Cardiomyocyte immunocytochemistry validation
Immunocytochemistry · Contractile Marker Expression
Cardiomyocyte voltage-gated currents
Electrophysiology · Voltage-Gated Currents
Cardiomyocyte action potential response
Electrophysiology · Action Potential Response
TM213 Differentiation Medium Kit

Generate Functional Cardiomyocytes In-House

For researchers who want control over differentiation, TM213 supports a streamlined workflow for generating cardiomyocytes from human embryonic stem cells.

Human cardiomyocyte differentiation roadmap

Maintain Control

Run the differentiation workflow in your own laboratory.

Scale to Your Needs

Generate cells based on project demand.

Reproducible Protocol

Use an optimized workflow designed for consistent differentiation.

Compatible Platform

Integrate with toxicity, disease modelling, and 3D culture workflows.

Promotional Cardiac Research Bundle

T5004 + 3DCelMatrix™ + Maintenance Medium Kit

A bundled cardiac workflow combines ready-to-use cardiomyocytes, extracellular-matrix support, and maintenance medium.

FAQ

Frequently Asked Questions

How quickly do the cardiomyocytes begin beating?

Spontaneous contractions are typically observed within 3–5 days after plating.

What validation is performed?

Cardiac identity is evaluated using molecular markers, immunocytochemistry, electrophysiology, sterility, and post-thaw viability checks.

How long are the cells functionally stable?

Functional performance is typically maintained for approximately 10–14 days in culture.

Need assistance?
Contact our experts for support and fast quotations.
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