sgRNA
The guide sequence directs Cas9 to the selected genomic target.
CRISPR fundamentals
CRISPR Knockout Cell Line Collection
What is CRISPR, and why does it matter for cell biology?
CRISPR-Cas9 uses a guide RNA to direct Cas9 to a selected DNA target. Knockout workflows rely on the cut and cellular repair process to disrupt gene function.
PAM site
A short adjacent DNA motif required for Cas9 recognition.
Double-strand break
Cas9 cuts both DNA strands at the programmed target site.
NHEJ repair
Error-prone repair often introduces indels that disrupt gene function.
Four-stage workflow
CRISPR knockout cell line generation
Guide design, delivery, cutting and repair, followed by clone screening and validation.
Portfolio highlights
Everything you need to knock out a gene, without running the edit yourself
2,000+ ready-to-use KO lines
A large pre-made inventory searchable by target and cell background.
Custom knockout generation
Project-specific KO development is available when the desired model is not in stock.
QC data included
Supporting validation helps researchers move into downstream assays sooner.
Faster than DIY
Avoid repeated guide, delivery, clone-isolation, and screening optimization.
Cell line insurance
Optional protection is available for selected cell-line handling issues.
Build vs. buy
Generating a knockout in-house vs. ordering one from abm
DIY
In-house CRISPR editing
- Design and validate guides
- Optimize Cas9 and guide delivery
- Isolate and screen clones
- Confirm the edit before the assay
abm
Ready-made or custom KO line
- Select an in-stock or custom combination
- Editing and clone screening handled upstream
- Receive supporting QC information
- Move directly into downstream assays
Find your model
Search the ready-to-use CRISPR KO collection
Product List
5 products
| Product Name | Cat. No. | Species | Cell Type |
|---|---|---|---|
 FLNC Stable Knockout Human A2780 Cell Line | T9503 | Human (H. sapiens) | Ovary |
 Human SMPDL3B & CCR3 CRISPR Double Knockout Jurkat Cell Line - BD384 | T3837 | Human (H. sapiens) | Blood |
 Immortalized Podocytes from COL4A3 Knockout Mice Cell Line (IMAS) | T0424 | Mouse (M. musculus) | Kidney |
 LIN28B CRISPR Knockout Human A2780 Stable Cell Line | T9506 | Human (H. sapiens) | Ovary |
 ULK1 CRISPR Knockout HeLa Stable Cell Line - Clone 3E1 | T6560 | Human (H. sapiens) | Cervix |