WEPRO7240 Series Dialysis Core Kit

Protein Expression Kits

Reagents for wheat germ cell-free expression using dialysis. Choose standard, GST-tag or His-tag formulations for the planned expression and purification workflow.

Overview

Features and applications

Dialysis can increase yield compared with the bilayer method, particularly for soluble targets. Use the kit to scale up production or obtain enough protein from one batch for multiple assays. Reaction volume depends on the dialysis device.

Required separately

Supply an expression template and a suitable dialysis device. The manual uses a 0.5 mL Slide-A-Lyzer MINI for 50 µL reactions and a 3 mL cassette for 3 mL reactions, both with a 10 kDa molecular weight cutoff.

Flow of operations

WEPRO7240 Series Dialysis Core Kit workflow

Product lineup

CFS-D7
WEPRO7240 Dialysis Core Kit
General-purpose kit for synthesizing a wide range of proteins.
CFS-D7G
WEPRO7240G Dialysis Core Kit
Provides higher purity for GST-tagged fusion proteins than the standard kit. The extract is precleared to reduce background binding to glutathione resin.
CFS-D7H
WEPRO7240H Dialysis Core Kit
Provides higher purity for His-tagged fusion proteins than the standard kit. The extract is precleared to reduce background binding to nickel resin.

Specifications

Kit capacity: 2 × 3 mL or 60 × 50 µL
Test-then-scale protocol: Up to 20 × 50 µL screening reactions, followed by up to 2 × 3 mL preparative reactions
Dialysis device: Required separately
Storage: −80 °C
1× SUB-AMIX SGC preparation: Use equal amounts of S1–S4 to prepare the working solution. Dilute each component into water to avoid precipitation.

CFS-D7 1 set

WEPRO7240 Dialysis Core Kit

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Component	Concentration	Volume	Quantity
Core kit · Transcription reagents
SP6 RNA polymerase	80 U/µL	15 µL	1
RNase inhibitor	80 U/µL	15 µL	1
NTP Mix	25 mM	120 µL	1
5× Transcription Buffer LM	5×	120 µL	1
Core kit · Translation reagents
WEPRO7240	240 OD	1 mL	1
Creatine kinase	20 mg/mL	20 µL	1
SUB-AMIX SGC S1	40×	600 µL	1
SUB-AMIX SGC S2	40×	600 µL	1
SUB-AMIX SGC S3	40×	600 µL	1
SUB-AMIX SGC S4	40×	600 µL	1
Included transcription reagent set
SP6 RNA polymerase	80 U/µL	15 µL	1
RNase inhibitor	80 U/µL	15 µL	1
NTP Mix	25 mM	120 µL	1
5× Transcription Buffer LM	5×	240 µL	1
External dialysis buffer reagents
SUB-AMIX SGC S1	40×	7 mL	1
SUB-AMIX SGC S2	40×	7 mL	1
SUB-AMIX SGC S3	40×	7 mL	1
SUB-AMIX SGC S4	40×	7 mL	1

CFS-D7G 1 set

WEPRO7240G Dialysis Core Kit

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Component	Concentration	Volume	Quantity
Core kit · Transcription reagents
SP6 RNA polymerase	80 U/µL	15 µL	1
RNase inhibitor	80 U/µL	15 µL	1
NTP Mix	25 mM	120 µL	1
5× Transcription Buffer LM	5×	120 µL	1
Core kit · Translation reagents
WEPRO7240G	240 OD	1 mL	1
Creatine kinase	20 mg/mL	20 µL	1
SUB-AMIX SGC S1	40×	600 µL	1
SUB-AMIX SGC S2	40×	600 µL	1
SUB-AMIX SGC S3	40×	600 µL	1
SUB-AMIX SGC S4	40×	600 µL	1
Included transcription reagent set
SP6 RNA polymerase	80 U/µL	15 µL	1
RNase inhibitor	80 U/µL	15 µL	1
NTP Mix	25 mM	120 µL	1
5× Transcription Buffer LM	5×	240 µL	1
External dialysis buffer reagents
SUB-AMIX SGC S1	40×	7 mL	1
SUB-AMIX SGC S2	40×	7 mL	1
SUB-AMIX SGC S3	40×	7 mL	1
SUB-AMIX SGC S4	40×	7 mL	1

CFS-D7H 1 set

WEPRO7240H Dialysis Core Kit

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Component	Concentration	Volume	Quantity
Core kit · Transcription reagents
SP6 RNA polymerase	80 U/µL	15 µL	1
RNase inhibitor	80 U/µL	15 µL	1
NTP Mix	25 mM	120 µL	1
5× Transcription Buffer LM	5×	120 µL	1
Core kit · Translation reagents
WEPRO7240H	240 OD	1 mL	1
Creatine kinase	20 mg/mL	20 µL	1
SUB-AMIX SGC S1	40×	600 µL	1
SUB-AMIX SGC S2	40×	600 µL	1
SUB-AMIX SGC S3	40×	600 µL	1
SUB-AMIX SGC S4	40×	600 µL	1
Included transcription reagent set
SP6 RNA polymerase	80 U/µL	15 µL	1
RNase inhibitor	80 U/µL	15 µL	1
NTP Mix	25 mM	120 µL	1
5× Transcription Buffer LM	5×	240 µL	1
External dialysis buffer reagents
SUB-AMIX SGC S1	40×	7 mL	1
SUB-AMIX SGC S2	40×	7 mL	1
SUB-AMIX SGC S3	40×	7 mL	1
SUB-AMIX SGC S4	40×	7 mL	1

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