Protein Expression Kits
WEPRO7240H Core Kit
Wheat germ expression kit with the WEPRO7240H extract for producing His-tagged proteins and preparing them for affinity purification.
- Cat.No
- CFS-C7H
- Pack size
- 1 set
Overview
His-tag purification
The WEPRO7240H extract reduces background from wheat germ proteins that bind during His-tag affinity purification. Use SDS-PAGE with Coomassie staining to check expression.
Recommended applications
Use for parallel soluble protein expression, screening, crystallography, interaction or functional assays, and co-expression of protein complexes. Choose a reaction scale based on the number of samples and the amount of protein required.
Flow of operations
Specifications
- Affinity tag
- His-tag
- Kit capacity
- 4 × 6 mL, 20 × 1.2 mL, or 96 × 226 µL
- Storage
- −80 °C
- 1× SUB-AMIX SGC preparation
- Use equal amounts of S1–S4 to prepare the working solution. Dilute each component into water to avoid precipitation.
Product composition
| Component | Concentration | Volume | Quantity |
|---|---|---|---|
| WEPRO7240H | 240 OD | 1 mL | 1 |
| SUB-AMIX SGC S1 | 40× | 600 µL | 1 |
| SUB-AMIX SGC S2 | 40× | 600 µL | 1 |
| SUB-AMIX SGC S3 | 40× | 600 µL | 1 |
| SUB-AMIX SGC S4 | 40× | 600 µL | 1 |
| Creatine kinase | 20 mg/mL | 20 µL | 1 |
| SP6 RNA polymerase | 80 U/µL | 15 µL | 1 |
| RNase inhibitor | 80 U/µL | 15 µL | 1 |
| NTP Mix | 25 mM | 120 µL | 1 |
| 5× Transcription Buffer LM | 5× | 240 µL | 1 |