G592Built-in gDNA removal
All-In-One 5X RT MasterMix with gDNA Removal
One-tube cDNA synthesis with integrated genomic DNA removal for workflows where gDNA could affect downstream PCR or qPCR.
View G592 →RT & RT-PCR
Browse reverse transcription products by reagent format and application, or use the product finder if you want help narrowing your options.
Reverse Transcription Products
Not sure? Use the Product FinderBrowse the RT Portfolio
Already know the type of workflow you need? Start here. Products are grouped by reagent format so you can compare similar options quickly.
Fast, ready-to-use setup
5X RT MasterMixes
For streamlined first-strand cDNA synthesis with fewer pipetting steps.
G590Streamlined RT
OneScript® Hot 5X RT MasterMix
Ready-to-use one-tube reverse transcription for cDNA synthesis when built-in genomic DNA removal is not required.
View G590 →Complete cDNA workflows
cDNA Synthesis Kits
For researchers who want a complete kit with separate reaction components.
G594Challenging RNA
OneScript® Hot cDNA Synthesis Kit
For structured, degraded, low-input, or inhibitor-containing RNA and workflows that benefit from higher RT temperatures.
View G594 →G236Routine cDNA
OneScript® Plus cDNA Synthesis Kit
Flexible first-strand cDNA synthesis for routine RNA samples and standard downstream applications.
View G236 →Build your own reaction
Reverse Transcriptase Enzymes
For custom RT reaction design when you want the reverse transcriptase enzyme as a separate component.
G593Challenging RNA
OneScript® Hot Reverse Transcriptase
High-temperature reverse transcriptase for custom reactions with structured, degraded, low-input, or inhibitor-containing RNA.
View G593 →G237Routine RT
OneScript® Plus Reverse Transcriptase
Flexible reverse transcriptase for routine custom RT reactions, including long RNA templates.
View G237 →Application-specific workflows
Specialized RT Products
For one-step RT-PCR or miRNA-specific cDNA synthesis.
G597One-step RT-PCR
MegaFi™ Pro One-Step RT-PCR
Combines reverse transcription and high-fidelity end-point PCR amplification in a single reaction.
View G597 →G898miRNA cDNA
miRNA All-In-One cDNA Synthesis Kit
Single-tube miRNA workflow combining polyadenylation and reverse transcription for miRNA cDNA synthesis.
View G898 →Compare All RT Products
Need help choosing?Reverse Transcription Portfolio at a Glance
Quickly compare all eight RT products by format and primary use.
| Product | Format | Best For |
|---|---|---|
| G592 All-In-One 5X RT MasterMix with gDNA Removal | 5X MasterMix | gDNA removal + cDNA |
| G590 OneScript® Hot 5X RT MasterMix | 5X MasterMix | Fast one-tube cDNA |
| G594 OneScript® Hot cDNA Synthesis Kit | cDNA Kit | Challenging RNA |
| G236 OneScript® Plus cDNA Synthesis Kit | cDNA Kit | Routine cDNA synthesis |
| G593 OneScript® Hot Reverse Transcriptase | Enzyme | Custom RT · challenging RNA |
| G237 OneScript® Plus Reverse Transcriptase | Enzyme | Custom RT · routine RNA |
| G597 MegaFi™ Pro One-Step RT-PCR | One-Step RT-PCR | RT + end-point PCR |
| G898 miRNA All-In-One cDNA Synthesis Kit | miRNA Kit | miRNA cDNA synthesis |
For more detailed guidance, use the Product Finder below.
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What Do “Hot” and “Plus” Mean?
OneScript® Hot and OneScript® Plus are two reverse transcriptase families. Hot is designed for higher-temperature RT and more challenging RNA, while Plus is a flexible choice for routine cDNA synthesis.
Higher-temperature RT for demanding RNA
Well suited to structured, degraded, low-input, or inhibitor-containing RNA and workflows that benefit from elevated reverse transcription temperatures.
Flexible RT for routine cDNA synthesis
Designed for reliable first-strand cDNA synthesis across routine RNA samples, including long RNA templates.
| Characteristic | OneScript® Hot | OneScript® Plus |
|---|---|---|
| Best suited for | Challenging, structured, degraded, low-input, or inhibitor-containing RNA | Routine and flexible cDNA synthesis |
| Thermostability range | 50–80°C | 50–55°C |
| Optimal temperature | 60°C | 50°C |
| Maximum cDNA elongation | 15 kb | 15 kb |
| Minimum sample input | 0.1 pg | 0.1 pg |
| Reaction time | 10 min | 15 min |
| Products in this family | G593, G594, G590, G592, G597 | G237, G236, G898 |
Performance by Reverse Transcriptase Family
Explore experimental data by reverse transcriptase family, then select the product or performance area most relevant to your workflow.
OneScript® Hot
High-Temperature RT for Challenging RNA
Designed for demanding samples and available in multiple formats, from enzyme-only reactions to ready-to-use MasterMix and one-step RT-PCR.
G593 · EnzymeG594 · cDNA KitG590 · 5X MasterMixG592 · 5X MasterMix + gDNA RemovalG597 · One-Step RT-PCR
G592 Performance
G592 formulation dataBuilt-In Genomic DNA Removal
G592 combines temperature-sensitive DNase and reverse transcription in one 5X MasterMix to reduce genomic DNA interference before downstream analysis.
qPCR
Reduce gDNA-Driven Early Amplification
Built-in gDNA removal helps reduce amplification from contaminating genomic DNA.
In the GAPDH assay shown, contaminating genomic DNA produced earlier amplification, which can complicate downstream interpretation. G592 integrates genomic DNA removal before reverse transcription in the same workflow to help reduce this source of signal.
Gel
Compare cDNA After gDNA Removal
Gel comparison shows cDNA after G592 gDNA removal versus a workflow without built-in removal.
This agarose gel compares a genomic-DNA-contaminated RNA sample processed with G592 against a competitor RT MasterMix without built-in genomic DNA removal. The figure illustrates the effect of removing genomic DNA before cDNA synthesis under the conditions shown.
G590 Stability
G590 stability reportReady-to-Use MasterMix Stability
G590 was evaluated after repeated freeze-thaw cycles and short-term storage at 4°C and 20°C using downstream qPCR.
Full functionality after 20 freeze-thaw cycles and 7 days at 4°C
A decrease in activity was reported after 3 days at 20°C, so the product should still be stored at −20°C as specified.
20× freeze-thaw: Pass4°C, 7 days: Pass20°C, 3 days: Decrease in activity
G593 Reverse Transcriptase
G593 enzyme dataOneScript® Hot Enzyme Performance
G593 performance data demonstrate high-temperature reverse transcription, low-input detection, inhibitor tolerance, enzyme stability, and competitive performance across multiple targets.
Temperature
50–80°C Demonstrated Range
High-temperature reverse transcription demonstrated across the 50–80°C range shown.
OneScript® Hot Reverse Transcriptase was evaluated at reverse-transcription temperatures from 50°C to 80°C. Successful cDNA synthesis across the demonstrated range supports workflows that benefit from elevated reverse-transcription temperatures, including challenging or structured RNA.
Sensitivity
Ultra-Low RNA Input
RT-qPCR detection demonstrated across the shown HEK293 total RNA dilution series.
RT-qPCR amplification was evaluated across the displayed HEK293 total RNA dilution series using G593. The data demonstrate amplification from progressively lower RNA inputs under the experimental conditions shown.
Inhibitor Tolerance
Performance With Common Inhibitors
cDNA synthesis remained strong across the tested inhibitor conditions.
G593 was tested in the presence of the isopropanol, BugBuster®, and heparin conditions shown in the figure. cDNA synthesis remained high across the tested inhibitor conditions relative to the experimental controls.
Enzyme Stability
Activity After 7 Days at 37°C
Enzyme activity remained detectable after extended storage at elevated temperatures.
G593 activity was assessed after storage at 4°C, 25°C, and 37°C for the indicated time periods. Amplification of both test targets remained visible after 7 days at 37°C under the conditions shown.
Comparison
Multi-Target Competitor Comparison
G593 was compared with SuperScript™ IV across multiple gel and qPCR targets.
G593 was compared with SuperScript™ IV using multiple targets measured by gel electrophoresis and qPCR. Under the conditions shown, G593 produced comparable target amplification across the tested assays.
G593 data characterize the reverse transcriptase enzyme. For complete formulations such as G594 and G597, refer to the individual product specifications and protocols.
OneScript® Plus
Flexible RT for Routine cDNA Synthesis
Designed for reliable first-strand cDNA synthesis across routine RNA samples, with support for long RNA templates and flexible reagent formats.
G237 · EnzymeG236 · cDNA KitG898 · miRNA cDNA Kit
G237 Reverse Transcriptase
G237 enzyme dataOneScript® Plus Enzyme Performance
G237 performance data demonstrate long-template cDNA synthesis and enzyme stability under the conditions shown.
Elongation
Long-Template cDNA Synthesis
Long-template cDNA synthesis demonstrated across targets up to approximately 15.6 kb.
OneScript® Plus Reverse Transcriptase was used to generate cDNA from human RNA targets spanning approximately 1.5 kb to 15.6 kb. The results demonstrate long-template reverse transcription across the fragment sizes shown.
Enzyme Stability
Activity After 7 Days at 37°C
G237 activity remained detectable after extended storage at elevated temperatures.
G237 activity was assessed after storage at 4°C, 25°C, and 37°C for the indicated time periods. Amplification of both test targets remained visible after 7 days at 37°C under the conditions shown.
G237 data characterize the reverse transcriptase enzyme. G236 provides a complete cDNA synthesis kit, while G898 is designed specifically for miRNA cDNA synthesis.
Reverse Transcription FAQs
Quick answers for choosing a chemistry, reagent format, or specialized workflow.
Should I choose OneScript® Hot or OneScript® Plus?
Choose OneScript® Hot for structured, degraded, low-input, or inhibitor-containing RNA and workflows that benefit from higher reverse transcription temperatures. Choose OneScript® Plus for routine and flexible cDNA synthesis.
Which product should I use if genomic DNA contamination is a concern?
G592 combines genomic DNA removal and reverse transcription in one 5X MasterMix, making it the direct starting point when gDNA may interfere with downstream PCR or qPCR.
When should I choose G590 instead of G592?
Choose G590 when you want a ready-to-use OneScript® Hot 5X RT MasterMix and built-in gDNA removal is not required. Choose G592 when gDNA removal is part of the workflow.
What is the difference between a cDNA kit and an enzyme-only product?
cDNA kits provide the supporting components for a complete reverse transcription workflow. Enzyme-only formats are better suited to custom reaction design.
Can I use total RNA or poly(A)+ mRNA?
Yes. OneScript® cDNA workflows support both total RNA and poly(A)+ mRNA.
Do I need to add a separate RNase inhibitor?
OneScript® reverse transcription products include RNaseOFF Ribonuclease Inhibitor, so an additional RNase inhibitor is generally not required for these formulations.
Is G597 for RT-qPCR?
G597 is designed for end-point one-step RT-PCR rather than one-step RT-qPCR.
What is different about G898 for miRNA?
G898 is a specialized miRNA cDNA synthesis workflow that combines polyadenylation and reverse transcription in a single-tube reaction.
Explore Reverse Transcription Resources
Go deeper into reverse transcription fundamentals, PCR workflow design, and product comparison tools.
Reverse Transcription
Review reverse transcription fundamentals, RT-PCR, end-point and real-time PCR, one-step versus two-step workflows, experimental notes, and sample protocols.
Read Reverse Transcription Guide →PCR: Variations to the System
Explore common PCR variations including multiplex, nested, asymmetric, touch-down, digital, site-directed mutagenesis, and isothermal PCR.
Explore PCR Variations →PCR/qPCR Competitor Cross Reference Tool
Compare the PCR or qPCR product you currently use with the corresponding abm option.
Open Cross Reference Tool →