Protein Expression Kits
Premium ONE Expression Kit
Premixed wheat germ reagents for small-scale, coupled transcription and translation. Use it to screen whether a target protein expresses before scaling up.
- Cat.No
- CFS-EDX-ONE
- Pack size
- 24 reactions
Overview
Template preparation
Start from an expression vector or a linear DNA template. The kit includes sense primers and a PCR protocol for testing expression of different protein fragments.
Expression and detection
The premixed WEPRO TT reagent couples transcription and translation. Fluorescent lysine labeling or Western blotting can detect suitable targets after a two-hour reaction. For SDS-PAGE with Coomassie staining, the manufacturer indicates 12–24 hours depending on protein yield.
Recommended applications
Screen a target for expression in the wheat germ system or prepare small quantities for testing and analysis.
Flow of operations
Specifications
- Reaction volume
- 55 µL
- Fluorescent labeling reagent
- Supplied for up to 8 reactions
- Storage
- −80 °C
Product composition
| Component | Concentration | Volume | Quantity |
|---|---|---|---|
| pEU-E01-MCS | 1 µg/µL | 5 µL | 1 |
| pEU-E01-DYKDDDDK-DHFR (positive control) | 15 ng/µL | 10 µL | 1 |
| SPU (PCR sense primer) | 1 µM | 100 µL | 1 |
| deSP6E01 (PCR sense primer) | 10 nM | 100 µL | 1 |
| WEPRO TTmix | — | 70 µL | 1 |
| SUB-AMIX TT | — | 1.3 mL | 1 |
| FluoroTect GreenLys tRNA | — | 4 µL | 1 |