Tris-Acetate-EDTA (TAE) Buffer

Thistle Scientific supplies TBE and TAE in powder and liquid form. Both TBE and TAE are widely used to separate nucleic acids in horizontal gel electrophoresis, and occasionally in vertical polyacrylamide gels. TBE has a higher buffering capacity than TAE, which is used for faster separations of linear double-stranded DNA.

Thistle Scientific's TBE is supplied in a bottle with enough powder to produce 10L of TBE buffer. The buffer may be opened as required and reconstituted in distilled water to make upto 10 litres of working solution. Buffers are also provided as ready-made 50x TAE and 10x TBE solutions in 1 and 4 litre volumes. These are ideal for laboratories running horizontal nucleic acid gels on a daily basis that require off-the-shelf working stock solutions.