Teaching Materials
Protein Expression Reagent Kit
Classroom kit for demonstrating transcription and translation from DNA. Students can observe protein production using green fluorescent protein.
Overview
Classroom experiment
Students follow DNA transcription and translation and use GFP fluorescence to observe protein synthesis. The kit supplies reagents for 20 students working in 10 pairs.
Flow of operations
Product lineup
CFS-EDU
Protein Expression Reagent Kit
1 set
Request a QuoteCFS-EDU-N
Protein Expression Reagent Kit without plastic consumables
1 set
Request a Quote
Specifications
- Storage
- −80 °C
- Separate reagent outside Japan
- Quant-iT RiboGreen RNA Reagent (R11491)
Product composition
| Component | Concentration | Volume | Quantity |
|---|---|---|---|
| Distilled water | — | 1,200 µL | 1 |
| Transcription buffer | — | 200 µL | 1 |
| Ribonucleotides | — | 100 µL | 1 |
| RNase | 10 mg/mL | 35 µL | 1 |
| RNase inhibitor | 10,000 U/mL | 68 µL | 1 |
| RNA polymerase | 10,000 U/mL | 99 µL | 1 |
| Plasmid DNA | 1 mg/mL | 66 µL | 1 |
| Wheat germ extract | — | 330 µL | 1 |
| Translation buffer with amino acids | — | 1,050 µL | 5 |
Plastic consumables · CFS-EDU
| Consumable | Quantity |
|---|---|
| 1.5 mL microtube | 170 |
| Plastic pipette tip (2–200 µL) | 4 × 96 |
| Clear flat-bottom tube with cap | 22 |