AffinityImmuno Products

Introduction

IL36RN encodes the interleukin-36 receptor antagonist, a secreted regulator that keeps inflammatory signaling in check at epithelial surfaces such as the skin. It works by occupying the IL-36 receptor chain (IL1RL2) so that the pro-inflammatory cytokines IL-36 alpha, beta, and gamma cannot dock there and recruit their signaling co-receptor, effectively muting a pathway that closely parallels classical IL-1 biology. This antagonist activity is thought to help dampen local innate immune responses, including reactions to fungal exposure, and may also engage an anti-inflammatory adaptor protein to reinforce the brake it puts on IL-36 signaling. Loss-of-function changes in this gene remove that brake and are the recognized cause of a severe, flare-prone pustular skin disease, underscoring how tightly IL-36 activity must be controlled for normal epidermal homeostasis.

Because loss-of-function IL36RN mutations are the recognized genetic cause of generalized pustular psoriasis — the same disease now treatable with the IL-36-receptor-blocking antibody spesolimab — this assay ties together genetic, biomarker, and drug-development research on the same pathway as our IL-36alpha and IL-36beta kits.

Principle of the Assay

This sandwich enzyme immunoassay kit uses a microtiter plate pre-coated with a capture antibody specific to Human IL36RN (FIL1δ). During incubation, IL36RN present in standards or samples is bound by the capture antibody, after which a biotin-conjugated detection antibody targeting a second epitope on Human IL36RN is added to each well. Avidin conjugated to horseradish peroxidase (HRP) is then introduced and binds the biotin-conjugated detection antibody. Upon addition of TMB substrate, a colorimetric signal develops exclusively in wells where Human IL36RN, the biotin-conjugated antibody, and avidin-HRP are all present. The enzymatic reaction is stopped by the addition of a sulphuric acid solution, and absorbance is measured spectrophotometrically at 450 nm (± 10 nm). Sample concentrations are determined by comparing sample optical density values against a standard curve.