AffinityImmuno Products

Introduction

IL-17F is an inflammatory cytokine most closely associated with the Th17 lineage of T-helper cells, although it is also produced by CD8+ T cells, NK cells, mast cells, and other innate lymphoid populations. It signals largely as a heterodimer with IL-17A through a shared IL-17RA/IL-17RC receptor complex, though it can also act as a homodimer via IL-17RC alone, triggering NF-kB and MAPK signaling that drives production of neutrophil-recruiting chemokines, antimicrobial peptides, and matrix-degrading enzymes. Through this pathway it recruits and activates neutrophils to help clear extracellular bacteria and fungi at epithelial surfaces, and it further protects mucosal barriers by prompting epithelial cells to produce defensin peptides. Beyond direct antimicrobial defense, IL-17F has been reported to restrain new blood vessel growth in endothelial cells even as it stimulates their output of other inflammatory mediators, and dysregulated IL-17F/IL-17A activity is linked to chronic inflammatory conditions including psoriasis and rheumatoid arthritis.

IL-17F’s antimicrobial defense role at mucosal surfaces, paired with its contribution to chronic inflammatory disease when dysregulated, makes it relevant both to psoriasis/rheumatoid arthritis research and to studies of IL-17A/IL-17F heterodimer biology now targeted by dual IL-17A/F biologics in dermatology and rheumatology drug development.

Principle of the Assay

This kit uses a sandwich enzyme immunoassay format in which the microtiter plate wells are pre-coated with a capture antibody specific to Human IL-17F. Standards or samples are added to the appropriate wells along with a biotin-conjugated detection antibody that also recognizes Human IL-17F. Avidin conjugated to horseradish peroxidase (HRP) is then added to each well and allowed to incubate, binding to the biotin on the detection antibody. Upon addition of TMB substrate solution, a colorimetric signal develops exclusively in wells where Human IL-17F, the biotin-conjugated antibody, and avidin-HRP are all present. The enzymatic reaction is stopped by the addition of a sulphuric acid solution, and the resulting color is measured spectrophotometrically at 450 nm (± 10 nm). Sample concentrations are determined by comparing the absorbance readings of each sample against a standard curve generated from the provided standards.