AffinityImmuno · ELISA Kit
hGH ADA (human growth hormone) ELISA
Cat.No
EL-141-878
96 wells
- Reactivity
- human
- Detection range
- 30 ng/mL -1920 ng/mL
- Sample type
- Serum Plasma
- Assay time
- 2.5 hours
- Antigen
- antibodies to hGH
- Detection minimum
- 30 ng/mL
Product Overview
Introduction
The AffinityImmuno hGH (Human Growth Hormone) ADA ELISA kit contains the components required for measuring antibodies to hGH in biological matrices such as human serum, plasma or cell culture supernatant.
Principle of the Assay
This assay employs the indirect enzyme immunoassay technique. hGH is coated onto a 96 well microplate. Calibrator and test samples prepared by dilution into assay buffer and are pipetted into the appropriate wells. Antibodies to hGH present in biological matrices are bound to the immobilized hGH. After washing away any unbound substances, antibodies
antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of antibodies to hGH present in test samples. The color development is stopped and the intensity of the color is measured.
Target background
Growth hormone (GH) or somatotropin, also known as human growth hormone (hGH or HGH) in its human form, is a peptide hormone that stimulates growth, cell reproduction, and cell regeneration in humans and other animals. It is thus important in human development. GH also stimulates production of IGF-1 and increases the concentration of glucose and free fatty acids
Specifications
- Target protein
- hGH (human growth hormone)
- Alternative names
- Somatropin
- Gene ID
- N/ap
- Application
- Measure anti-hGH antibodies
- Reactivity
- human
- Detection range
- 30 ng/mL -1920 ng/mL
- Sample type
- Serum Plasma
- Sample volume
- 15ul
- Assay time
- 2.5 hours
- Assay precision
- Intra-assay coefficient variation (CV) <20%, inter-assay CV <20%
- Storage
- Stable at -20°C
- Size
- 96 wells
- Antigen
- antibodies to hGH
- Detection minimum
- 30 ng/mL
- Method
- Direct sandwich ELISA
- Detection method
- Peroxidase / OD450
- Plate format
- Strip
- Specificity
- anti-hGH antibodies
- Preservative
- none
- Alternative name
- Somatropin
Kit Components
- Coated microtiter plate, 96 wells (12x8 strips) 1 Calibrator diluent 1.8 ml Calibrator (1.15mg/mL) 12 μl 10X wash buffer 50 ml Assay buffer 50 ml 1000X detection reagent 17 μl TMB 12 ml TMB stop solution
Assay Information
Reagent preparation
Prepare only the appropriate amount of required reagent on the day of use. Store all reagents as per instructions stated on the label. 1. Wash Buffer (1X) Preparation Dilute wash buffer concentrate with deionized water 1/10 before use (for example add 20mL concentrate to 180mL deionized water). Mix well. 2. Detection Reagent (1X) Preparation: Dilute detection reagent with assay buffer 1/1000 before use (for example add 11μl concentrate to 11ml of assay buffer). Mix well. The following is an example calibrator curve.
Results calculation
1. Construct a standard curve by plotting the absorbance obtained from each standard against concentration. Use a 4 or 5 parameter curve fit. Alternatively a log-log curve fit may be used. 2. The concentration of the unknowns can be read directly from this standard curve using the absorbance value for each sample. 3. Any sample undiluted or diluted still reading greater than the highest standard should be diluted appropriately with assay buffer and retested. If the samples have been diluted, the concentration determined from the standard curve must be multiplied by the dilution factor.
Sample collection
This kit is compatible with EDTA-plasma, heparinplasma and serum samples. Samples can be stored at or below -20°C for up to 1 year.
Sample preparation
Dilute calibrators and test samples 1/100 with assay buffer (for example add 5µL of prepared calibrator or sample to 495µL of assay buffer). Mix well. Do not store diluted samples.