AffinityImmuno · ELISA Kit
GCSF-PEG ADA ELISA kit | EL-141-73196–GCSF-PEG-ADA
Cat.No
EL-141-73196--GCSF-PEG-ADA
96 wells
- Reactivity
- human
- Detection range
- 45 ng/mL -1440 ng/mL
- Sample type
- Serum Plasma
- Assay time
- 2.5 hours
Product Overview
Introduction
The AffinityImmuno GCSF-PEG ADA ELISA kit contains the components required for measuring antibodies to GCSF-PEG in biological matrices such as human serum, plasma or cell culture.
Principle of the Assay
This assay employs the indirect enzyme immunoassay technique. GCSF-PEG is coated onto a 96 well microplate. Calibrator and test samples are prepared by dilution into assay buffer and are pipetted into the appropriate wells. Antibodies to GCSF-PEG present in biological matrices are bound to the immobilized GCSF-PEG. After washing away any unbound substances, antibody-enzyme reagent and a substrate solution is added to the wells for color development is proportional to the amount of antibodies to GCSF- PEG present in the calibrator and test samples. The color development is stopped and the intensity of the color is measured.
Related assay
Measuring drug levels rather than immunogenicity? See the GCSF-PEG pharmacokinetic ELISA kit.
Related reading
For context on drug-specific anti-drug antibody (ADA) monitoring during PEGylated-drug trials, see our 2026 PEG immunogenicity review.
Specifications
- Target protein
- PEG-GCSF (Granulocyte colony -stimulating factor)
- Alternative names
- PEG-filgrastim
- Gene ID
- N/ap
- Application
- Measure anti-PEG-GCSF antibodies
- Reactivity
- human
- Detection range
- 45 ng/mL -1440 ng/mL
- Sample type
- Serum Plasma
- Sample volume
- 15ul
- Assay time
- 2.5 hours
- Assay precision
- <20%, <20%
- Storage
- Stable at -20C for 1 year
- Size
- 96 wells
Kit Components
- Coated microtiter plate, 96 wells
- Calibrator diluent. - 1.8ml
- Calibrator (250 ug/mL) - 12ul
- 10X wash buffer - 25ml
- Assay buffer - 50ml
- 1000X detection reagent - 17ul
- TMB - 12ml
- TMB stop solution - 12ml
- Plate sealers - 3