LEARNING RESOURCES

Everybody Loves CRISPR Chopping! Those Edits are Fast as Lightning

Our comprehensive CRISPR resources provides everything you need to get your feet wet with this innovative gene editing platform. From introductory materials about the theory and history, to practical guides on how to design your CRISPR gene editing experiment, to protocols and case studies - get the confidence you need to get started on your own gene editing project!

Popular Topics:

• Introduction - essential principles and theories
• Tools and Methods - Cas9 and sgRNA delivery
• CRISPR Screening and Validation - heterozygous, bialleilic, or homozygous editing
• Gene regulation with dCas9 - Mutant Cas9 for gene activation or repression

View Resources

Transcriptional Factors and dCas9 Regulate Expression

Go beyond the classic CRISPR knockout: a catalytically dead Cas9 (dCas9) can fuse with  transcriptional activators or repressors and localize to desired binding sites to regulate gene expression. Learn more about it!

Watch Video

Gain Hands-on Experience: CRISPR KO Teaching Kit

Students can complete their first CRISPR experiment and knockout the chloramphenicol resistance gene in E. coli (previously introduced into the genome at the yeeR locus). Available as Cat. No. K099.

Watch Video

CRISPR Knockout Manual

Our free 39-page CRISPR knockout manual explains how to use CRISPR Cas9 to knockout a gene in a mammalian system. Includes KO protocol, sgRNA design and data validation tips, as well as knockout case studies!

Download Manual

Sign-up for CRISPR Crash Course

This free 4-week course provides short guides, protocols, and videos outlining how to set up a CRISPR knockout experiment! You'll receive a new lesson every week and a quiz to test yourself!

Free Sign-up

Find Other CRISPR Tools:

·         Webinar: Guide to Becoming a CRISPR Cas9 Expert

·         Knockout/knock-in Case Studies

·         Experiment Design Tool