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[abm] CRISPR Cas9+sgRNA All-in-One Vector/Virus Collection

itsbio · 2022-01-10

 

CRISPR-All-In-One Expression System

 

POPULAR COLLECTION

CRISPR All-in-One Expression Systems

 

Enjoy the convenience of expressing Cas9 protein and guide RNA from one single vector! abm offers a comprehensive library of ready-to-use CRISPR All-in-One knockout constructs that target mouse, rat, and human genes.
Why choose the CRISPR All-in-One expression system? 
  • Flexible co-expression of guide RNA with spCas9 nuclease (lentivirus), or with saCas9 nuclease (AAV)
  • Easy selection using puromycin or substitute with GFP marker
  • Saves time with single transfection, transduction, and antibiotic

 

Search CRISPR Collection  

 

Workflow for CRISPR All-in-One Expression Systems

 

 

CRISPR Lenti/AAV Vector/Virus Designs:

 

All-in-One Knockout (spCas9) Lentivectors

pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro

Construct

 

pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro

All-in-One Knockout (saCas9) AAV Vectors

pAAV-PGK-saCas9-U6-sgRNAsa-hGH-amp

Set of 3 

 

pAAV-PGK-saCas9-U6-sgRNAsa-Amp

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