ABM (Applied Biological Materials Inc.)

   Origin : Canada

  iSPC Lentiviruses

 

EF1メ Polytronic Yamanaka Factor Lentivirus

 

Cat.No.: G352
Quantity: 200ul
Titer: 1x107 cfu/mL

 

 

Data Sheet

 

Safety

WGK Germany 3.

Vector

pLenti-III-EF1メ-Polytronic-OSKM

Promoter

EF1メ

Gene

Oct4, Sox2, KLF4 and Myc

Organism

Human

Accession #

Q01860, P48431, O43474 and P01106

Description

The polycistronic lentivirus expresses all of the four human transcription factors (hOSKM of Yamanaka set: Oct4, Sox2, KLF4 or Myc) within a single expression cassette under the control of EF1メ promoter. Each of the factor's ORF is separated by a unique 2A peptide (P2A, T2A, and E2A) sequence, which allows for the equivalent expression of all four transcription factors from a single vector. The expression of all factors from a single virus minimizes the number of proviral integrations required for successful reprogramming. This also reduces the risk of insertional mutagenesis effects in the resultant induced pluripotent stem (iPS) cells when compared to those generated using four independent viruses. Also, the virus is provided as concentrated and VSV-G pseudotyped stock capable of transducing both dividing and non-dividing cells. A control GFP lentivirus is included for the easy tracking of gene transduction efficiency in your target cells.

Functions

The recombinant polycistronic lentivirus is provided as a VSV-G pseudotyped and concentrated virus stock capable of infecting both dividing and non-dividing cells. The expression of these transcription factors has been shown to reprogram adult human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem cell (iPSCs).

Quality Control

Viral titer (cfu/mL) is determined by quantitative RT-PCR of viral stock preparation. Transcription factor protein expression are verified by immunocytochemistry and Western Blot analysis of transduced 293 cells. All of ABMâ∽≒s viral preparations are tested to be free of bacteria and other microbials.

Titer

1x107 cfu/mL

Storage

-70°C

Shipping

On Dry Ice

References

Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Takahashi et al. Cell. 2007 Nov 30;131(5):861-72.

Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Takahaski and Yamanaka. Cell. 2006 Aug 25;126(4):663-76. Epub 2006 Aug 10.