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[abm] Cas9 Proteins 프린트 
이츠바이오  Email [2018-10-12 09:55:00]  HIT : 170  

Applied Biological Materials Inc. ABM

 Cas9 Proteins

 

This method has several advantages over other Cas9 delivery methods:

  • There is no transcription or translation required so editing is much more rapid and transient
  • Cas9 proteins are compatible with a wide range of organisms without consideration such as codon optimization or promoter compatibility
  • No plasmid or virus means no risk of Cas9 or sgRNA integration into the genome

Cas9 proteins and sgRNA can also be used for cutting DNA in vitro. Applications for this technique include pre-screening of sgRNA candidates on PCR amplified versions of the target gene of interest, or even cutting of plasmids where no restriction site is available.

 

spCas9 Protein
Product Cat No. Quantity
Cas9 Nuclease Protein, E. coli K008 8.0µg (50pmol) 
Volume: 50µL, 1000 nM, 160 µg/ml
Cas9 Nuclease NLS Protein, E. coli K030 8.0µg (50pmol) 
Volume: 50µL, 1000 nM, 160 µg/ml
Cas9 Nickase D10A Protein, E. coli K032 8.0µg (50pmol) 
Volume: 50µL, 1000 nM, 160 µg/ml
Cas9 Nickase D10A NLS Protein, E. coli K034 8.0µg (50pmol) 
Volume: 50µL, 1000 nM, 160 µg/ml
Cas9 Nickase H840A Protein, E. coli K036 8.0µg (50pmol) 
Volume: 50µL, 1000 nM, 160 µg/ml
Cas9 Nickase H840A NLS Protein, E. coli K038 8.0µg (50pmol) 
Volume: 50µL, 1000 nM, 160 µg/ml
Cas9 Null Mutant Protein, E. coli K040 8.0µg (50pmol) 
Volume: 50µL, 1000 nM, 160 µg/ml
Cas9 Null Mutant NLS Protein, E. coli K042 8.0µg (50pmol) 
Volume: 50µL, 1000 nM, 160 µg/ml
 
High Concentration spCas9 Proteins
Product Cat No. Quantity
Cas9 Nuclease Protein, E. coli K108 40µg (250pmol) 
Volume: 25µL, 10 µM, 1.60 mg/ml
Cas9 Nuclease NLS Protein, E. coli K130 40µg (250pmol) 
Volume: 25µL, 10 µM, 1.60 mg/ml
Cas9 Nickase D10A Protein, E. coli K132 40µg (250pmol) 
Volume: 25µL, 10 µM, 1.60 mg/ml
Cas9 Nickase D10A NLS Protein, E. coli K134 40µg (250pmol) 
Volume: 25µL, 10 µM, 1.60 mg/ml
Cas9 Nickase H840A Protein, E. coli K136 40µg (250pmol) 
Volume: 25µL, 10 µM, 1.60 mg/ml
Cas9 Nickase H840A NLS Protein, E. coli K138 40µg (250pmol) 
Volume: 25µL, 10 µM, 1.60 mg/ml
Cas9 Null Mutant Protein, E. coli K140 40µg (250pmol) 
Volume: 25µL, 10 µM, 1.60 mg/ml
Cas9 Null Mutant NLS Protein, E. coli K142 40µg (250pmol) 
Volume: 25µL, 10 µM, 1.60 mg/ml
 
spCas9 GFP Proteins
Product Cat No. Quantity
Cas9 Nuclease GFP NLS Protein, E. coli K048 9.5µg (50pmol)
Volume: 50µL, 1000 nM, 188 µg/ml
Cas9 Nuclease GFP NLS Protein, E. coli K148 47µg (250pmol) 
Volume: 25µL, 10 µM, 1.88 mg/ml
Cas9 D10A Nickase GFP NLS Protein, E. coli K049 9.5µg (50pmol)
Volume: 50µL, 1000 nM, 188 µg/ml
Cas9 D10A Nickase GFP NLS Protein, E. coli K149 47µg (250pmol) 
Volume: 25µL, 10 µM, 1.88 mg/ml
Cas9 Null Mutant GFP NLS Protein, E. coli K086 9.5µg (50pmol)
Volume: 50µL, 1000 nM, 188 µg/ml
Cas9 Null Mutant GFP NLS Protein, E. coli K186 47µg (250pmol) 
Volume: 25µL, 10 µM, 1.88 mg/ml
 
saCas9 Proteins
Product Cat No. Quantity
saCas9 Nuclease Protein, E. coli K044 6.5µg (50pmol) 
Volume: 50µL, 1000 nM, 130 µg/ml
saCas9 Nuclease NLS Protein, E. coli K045 6.5µg (50pmol) 
Volume: 50µL, 1000 nM, 130 µg/ml
saCas9 Null Mutant Protein, E. coli K046 6.5µg (50pmol) 
Volume: 50µL, 1000 nM, 130 µg/ml
saCas9 Null Mutant NLS Protein, E. coli K047 6.5µg (50pmol) 
Volume: 50µL, 1000 nM, 130 µg/ml
 
High Concentration saCas9 Proteins
Product Cat No. Quantity
saCas9 Nuclease Protein, E. coli K144 32.5µg (250pmol)
Volume: 25µL, 10 µM, 1.30 mg/ml
saCas9 Nuclease NLS Protein, E. coli K145 32.5µg (250pmol)
Volume: 25µL, 10 µM, 1.30 mg/ml
saCas9 Null Mutant Protein, E. coli K146 32.5µg (250pmol)
Volume: 25µL, 10 µM, 1.30 mg/ml
saCas9 Null Mutant NLS Protein, E. coli K147 32.5µg (250pmol)
Volume: 25µL, 10 µM, 1.30 mg/ml
 
sgRNA Synthesis and Screening Kits
Product Cat No. Quantity
GeneCraft-R Express CRISPR sgRNA Synthesis Kit (S. pyogenes) G948 25 Reactions
GeneCraft-R Classic CRISPR sgRNA Synthesis Kit (S. pyogenes) G952 25 Reactions
GeneCraft-R CRISPR sgRNA Screening Kit (S. pyogenes) G953 25 Reactions
GeneCraft-R Express CRISPR sgRNA Synthesis and Screening Kit (S. pyogenes) G954 25 Reactions
 
Other Cas9 Related Products
Product Cat No. Quantity
CRISPR Genomic Cleavage Detection Kit G932 25 Reactions
OneScribe T7 Transcription Kit E081 50 Reactions
GFP Purified Protein 000033P 50 μg
RFP Purified Protein 000035P 500 μg
Anti-Cas9 Antibody Y300079 100 μl

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▼ CRISPR sgRNA

▼ Cas9 System

▼ dCas9 Double Mutant

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