{"id":7568,"date":"2021-01-08T09:52:21","date_gmt":"2021-01-08T00:52:21","guid":{"rendered":"http:\/\/itsbio.co.kr\/?page_id=7568"},"modified":"2021-01-08T09:54:35","modified_gmt":"2021-01-08T00:54:35","slug":"trastuzumabherceptin-pharmacokinetic-elisa","status":"publish","type":"page","link":"http:\/\/itsbio.co.kr\/?page_id=7568","title":{"rendered":"Trastuzumab(Herceptin\u00ae) Pharmacokinetic ELISA"},"content":{"rendered":"

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HOME<\/a> \"\" Affinityimmuno<\/a> \"\"ELISAs<\/a><\/p>\n<\/div>\n

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Trastuzumab(Herceptin\u00ae) Pharmacokinetic ELISA<\/strong><\/span><\/h1>\n<\/div>\n

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CATALOG NUMBER :\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 <\/span><\/span>EL-1611-201<\/span><\/strong><\/p>\n

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Trastuzumab ELISA Herceptin ELISA Pharmacokinetic ELISA Biosimilar<\/span><\/h3>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”3.25″ background_size=”initial” background_position=”top_left” background_repeat=”repeat”][et_pb_column type=”4_4″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.6.3″ vertical_offset_tablet=”0″ horizontal_offset_tablet=”0″ custom_margin=”||71px|||” z_index_tablet=”0″ text_text_shadow_horizontal_length_tablet=”0px” text_text_shadow_vertical_length_tablet=”0px” text_text_shadow_blur_strength_tablet=”1px” link_text_shadow_horizontal_length_tablet=”0px” link_text_shadow_vertical_length_tablet=”0px” link_text_shadow_blur_strength_tablet=”1px” ul_text_shadow_horizontal_length_tablet=”0px” ul_text_shadow_vertical_length_tablet=”0px” ul_text_shadow_blur_strength_tablet=”1px” ol_text_shadow_horizontal_length_tablet=”0px” ol_text_shadow_vertical_length_tablet=”0px” ol_text_shadow_blur_strength_tablet=”1px” quote_text_shadow_horizontal_length_tablet=”0px” quote_text_shadow_vertical_length_tablet=”0px” quote_text_shadow_blur_strength_tablet=”1px” header_text_shadow_horizontal_length_tablet=”0px” header_text_shadow_vertical_length_tablet=”0px” header_text_shadow_blur_strength_tablet=”1px” header_2_text_shadow_horizontal_length_tablet=”0px” header_2_text_shadow_vertical_length_tablet=”0px” header_2_text_shadow_blur_strength_tablet=”1px” header_3_text_shadow_horizontal_length_tablet=”0px” header_3_text_shadow_vertical_length_tablet=”0px” header_3_text_shadow_blur_strength_tablet=”1px” header_4_text_shadow_horizontal_length_tablet=”0px” header_4_text_shadow_vertical_length_tablet=”0px” header_4_text_shadow_blur_strength_tablet=”1px” header_5_text_shadow_horizontal_length_tablet=”0px” header_5_text_shadow_vertical_length_tablet=”0px” header_5_text_shadow_blur_strength_tablet=”1px” header_6_text_shadow_horizontal_length_tablet=”0px” header_6_text_shadow_vertical_length_tablet=”0px” header_6_text_shadow_blur_strength_tablet=”1px” box_shadow_horizontal_tablet=”0px” box_shadow_vertical_tablet=”0px” box_shadow_blur_tablet=”40px” box_shadow_spread_tablet=”0px”]<\/p>\n

INTRODUCTION<\/strong><\/span><\/h5>\n

\u00a0<\/p>\n

Trastuzumab (trade name Herceptin\u00ae) is indicated for the treatment of HER2-positive breast cancer, and adjuvant therapies for metastatic gastric cancer and gastroesophageal cancer. Serum concentration of trastuzumab may predict some clinical outcome during therapy. It is also possible that the surveillance of circulating trastuzumab concentration during therapy represents a direct factor for immunogenicity and some other side effects. Identification of biomarkers and risk factors for adverse drug reactions that might be related to serum concentrations, and maintaining the effective minimum concentration of trastuzumab in order to potentially avoid some side effects, might be beneficial using a reliable method.<\/span><\/p>\n

\u00a0<\/p>\n

The Trastuzumab ELISA kit is designed to measure trastuzumab with high specificity and enhanced sensitivity. The assay design utilizes a pair of antibodies that allows the detection of the whole trastuzumab molecule in biological matrices.<\/span><\/p>\n

\u00a0<\/p>\n

PRINCIPLE OF THE ASSAY<\/strong><\/span><\/h5>\n

\u00a0<\/p>\n

This assay employs the sandwich enzyme immunoassay\u00a0technique. Anti-Trastuzumab is coated onto a 96 well microplate. Calibrator, quality control samples\u00a0and test samples are pipetted into the appropriate\u00a0wells. Trastuzumab\u00a0present in\u00a0biological matrices is bound by the immobilized capture antibody. After washing away\u00a0any unbound substances, enzyme linked detection antibody is added to the wells.\u00a0The plate is washed to remove any unbound antibody-enzyme reagent and a\u00a0substrate solution is added to the wells for color development. The color development\u00a0is proportional to the amount of Trastuzumab\u00a0present in test samples.<\/span><\/p>\n

\"\"<\/span><\/p>\n

\u00a0<\/p>\n

The standard curve was generated with the supplied calibrator using the recommended<\/span>
conditions. Each sample was run with 6 replicates. Intra-assay coefficient of variance is <10%.<\/span><\/p>\n

[\/et_pb_text][et_pb_text _builder_version=”4.6.3″ vertical_offset_tablet=”0″ horizontal_offset_tablet=”0″ z_index_tablet=”0″ text_text_shadow_horizontal_length_tablet=”0px” text_text_shadow_vertical_length_tablet=”0px” text_text_shadow_blur_strength_tablet=”1px” link_text_shadow_horizontal_length_tablet=”0px” link_text_shadow_vertical_length_tablet=”0px” link_text_shadow_blur_strength_tablet=”1px” ul_text_shadow_horizontal_length_tablet=”0px” ul_text_shadow_vertical_length_tablet=”0px” ul_text_shadow_blur_strength_tablet=”1px” ol_text_shadow_horizontal_length_tablet=”0px” ol_text_shadow_vertical_length_tablet=”0px” ol_text_shadow_blur_strength_tablet=”1px” quote_text_shadow_horizontal_length_tablet=”0px” quote_text_shadow_vertical_length_tablet=”0px” quote_text_shadow_blur_strength_tablet=”1px” header_text_shadow_horizontal_length_tablet=”0px” header_text_shadow_vertical_length_tablet=”0px” header_text_shadow_blur_strength_tablet=”1px” header_2_text_shadow_horizontal_length_tablet=”0px” header_2_text_shadow_vertical_length_tablet=”0px” header_2_text_shadow_blur_strength_tablet=”1px” header_3_text_shadow_horizontal_length_tablet=”0px” header_3_text_shadow_vertical_length_tablet=”0px” header_3_text_shadow_blur_strength_tablet=”1px” header_4_text_shadow_horizontal_length_tablet=”0px” header_4_text_shadow_vertical_length_tablet=”0px” header_4_text_shadow_blur_strength_tablet=”1px” header_5_text_shadow_horizontal_length_tablet=”0px” header_5_text_shadow_vertical_length_tablet=”0px” header_5_text_shadow_blur_strength_tablet=”1px” header_6_text_shadow_horizontal_length_tablet=”0px” header_6_text_shadow_vertical_length_tablet=”0px” header_6_text_shadow_blur_strength_tablet=”1px” box_shadow_horizontal_tablet=”0px” box_shadow_vertical_tablet=”0px” box_shadow_blur_tablet=”40px” box_shadow_spread_tablet=”0px”]<\/p>\n

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\u00a0<\/h3>\n

Product Specifications<\/span><\/strong><\/h3>\n

\u00a0<\/p>\n<\/div>\n<\/div>\n

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\u00a0<\/div>\n
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  • \n
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    \n
    APPLICATION(S) : measure\u00a0free Tocilizumab<\/span><\/span><\/h6>\n

    \u00a0<\/p>\n<\/div>\n<\/div>\n<\/li>\n

  • \n
    \n
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    TARGET PROTEIN : herceptin<\/span><\/span><\/h6>\n

    \u00a0<\/p>\n<\/div>\n<\/div>\n<\/li>\n

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    ALTERNATIVE NAME OF TARGET : trastuzumab<\/span><\/span><\/h6>\n

    \u00a0<\/p>\n<\/div>\n<\/div>\n<\/li>\n

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    \u00a0SIZE : <\/span>96 wells<\/span><\/h6>\n

    \u00a0<\/p>\n<\/div>\n<\/div>\n<\/li>\n

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    SENSITIVITY : <\/span>detection limit 1.5 ng\/mL<\/span><\/h6>\n

    \u00a0<\/p>\n<\/div>\n<\/div>\n<\/li>\n

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    DETECTION METHOD : <\/span>Peroxidase \/ OD450<\/span><\/h6>\n

    \u00a0<\/p>\n<\/div>\n<\/div>\n<\/li>\n

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    PRECISION : <\/span>Intra-assay coefficient of variation (CV) <10%. Inter-assay CV was <10%<\/span><\/h6>\n

    \u00a0<\/p>\n<\/div>\n<\/div>\n<\/li>\n

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    DETECTION LIMIT : 1<\/span>.25ng\/ml<\/span><\/h6>\n

    \u00a0<\/p>\n<\/div>\n<\/div>\n<\/li>\n

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    STORAGE CONDITIONS : <\/span>Stable at -20\u00b0C for 1 year<\/span><\/h6>\n

    \u00a0<\/p>\n<\/div>\n<\/div>\n<\/li>\n

  • \n
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    SAFETY WARNINGS AND PRECAUTIONS : <\/span>Research Use Only. Follow instructions.<\/span><\/h6>\n

    \u00a0<\/p>\n<\/div>\n<\/div>\n<\/li>\n<\/ul>\n

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    \n
    EACH KIT INCLUDES :<\/span><\/h6>\n

    \u00a0<\/p>\n

    – Coated microtiter plate\u00a0<\/span><\/p>\n

    – 96 wells Calibrator diluent : 1.8ml\u00a0<\/span><\/p>\n

    – Calibrator (1000 ug\/mL) : 12ul\u00a0<\/span><\/p>\n

    – 10X wash buffer : 25ml\u00a0<\/span><\/p>\n

    – Assay buffer \u00a0: 50ml\u00a0<\/span><\/p>\n

    – 1000X detection reagent : 17ul\u00a0<\/span><\/p>\n

    – TMB : 12ml\u00a0<\/span><\/p>\n

    – TMB stop solution : 12ml\u00a0<\/span><\/p>\n

    – Plate sealers : 3<\/span><\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

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