{"id":7515,"date":"2021-01-07T16:50:52","date_gmt":"2021-01-07T07:50:52","guid":{"rendered":"http:\/\/itsbio.co.kr\/?page_id=7515"},"modified":"2021-01-07T16:53:30","modified_gmt":"2021-01-07T07:53:30","slug":"anti-peg-antibody-elisa-human-igm-specific","status":"publish","type":"page","link":"http:\/\/itsbio.co.kr\/?page_id=7515","title":{"rendered":"Anti PEG antibody ELISA (human IgM specific)"},"content":{"rendered":"

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HOME<\/a> \"\" Affinityimmuno<\/a> \"\"ELISAs<\/a><\/p>\n<\/div>\n

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Anti PEG antibody ELISA (human IgM specific)<\/strong><\/span><\/h1>\n<\/div>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section bb_built=”1″ _builder_version=”4.4.2″ vertical_offset_tablet=”0″ horizontal_offset_tablet=”0″ custom_margin=”1px|||||” z_index_tablet=”0″ border_width_bottom=”5px” box_shadow_horizontal_tablet=”0px” box_shadow_vertical_tablet=”0px” box_shadow_blur_tablet=”40px” box_shadow_spread_tablet=”0px” prev_background_color=”#000000″ next_background_color=”#000000″][et_pb_row column_structure=”1_2,1_2″ _builder_version=”3.25″ background_size=”initial” background_position=”top_left” background_repeat=”repeat”][et_pb_column type=”1_2″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_image src=”http:\/\/itsbio.co.kr\/wp-content\/uploads\/2021\/01\/ELISAs.png” align=”center” _builder_version=”4.6.3″ vertical_offset_tablet=”0″ horizontal_offset_tablet=”0″ width=”100%” max_width=”100%” custom_padding=”0px||0px|||” z_index_tablet=”0″ box_shadow_horizontal_tablet=”0px” box_shadow_vertical_tablet=”0px” box_shadow_blur_tablet=”40px” box_shadow_spread_tablet=”0px” \/][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.6.3″ vertical_offset_tablet=”0″ horizontal_offset_tablet=”0″ z_index_tablet=”0″ text_text_shadow_horizontal_length_tablet=”0px” text_text_shadow_vertical_length_tablet=”0px” text_text_shadow_blur_strength_tablet=”1px” link_text_shadow_horizontal_length_tablet=”0px” link_text_shadow_vertical_length_tablet=”0px” link_text_shadow_blur_strength_tablet=”1px” ul_text_shadow_horizontal_length_tablet=”0px” ul_text_shadow_vertical_length_tablet=”0px” ul_text_shadow_blur_strength_tablet=”1px” ol_text_shadow_horizontal_length_tablet=”0px” ol_text_shadow_vertical_length_tablet=”0px” ol_text_shadow_blur_strength_tablet=”1px” quote_text_shadow_horizontal_length_tablet=”0px” quote_text_shadow_vertical_length_tablet=”0px” quote_text_shadow_blur_strength_tablet=”1px” header_text_shadow_horizontal_length_tablet=”0px” header_text_shadow_vertical_length_tablet=”0px” header_text_shadow_blur_strength_tablet=”1px” header_2_text_shadow_horizontal_length_tablet=”0px” header_2_text_shadow_vertical_length_tablet=”0px” header_2_text_shadow_blur_strength_tablet=”1px” header_3_text_shadow_horizontal_length_tablet=”0px” header_3_text_shadow_vertical_length_tablet=”0px” header_3_text_shadow_blur_strength_tablet=”1px” header_4_text_shadow_horizontal_length_tablet=”0px” header_4_text_shadow_vertical_length_tablet=”0px” header_4_text_shadow_blur_strength_tablet=”1px” header_5_text_shadow_horizontal_length_tablet=”0px” header_5_text_shadow_vertical_length_tablet=”0px” header_5_text_shadow_blur_strength_tablet=”1px” header_6_text_shadow_horizontal_length_tablet=”0px” header_6_text_shadow_vertical_length_tablet=”0px” header_6_text_shadow_blur_strength_tablet=”1px” box_shadow_horizontal_tablet=”0px” box_shadow_vertical_tablet=”0px” box_shadow_blur_tablet=”40px” box_shadow_spread_tablet=”0px”]<\/p>\n

CATALOG NUMBER :\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 <\/span><\/span>EL-141-PEG-hIGM<\/span><\/strong><\/p>\n

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This immunogenicity assay measures the presence of human IgM in serum or plasma that bind to immobilized PEG using the direct ELISA technique.<\/span><\/h3>\n

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INTRODUCTION<\/strong><\/span><\/h5>\n

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Polyethylene glycol (PEG) chains are often used to modify therapeutic biologic agents in order to prolong the circulating half-life of the modified protein by slowing proteolytic degradation. It has been reported that repeat injections of PEGylated proteins can induce anti-PEG antibodies. Anti-PEG antibodies can result in rapid clearance and decreased drug efficacy (accelerated blood clearance, or ABC, phenomenon).<\/span><\/p>\n

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PRINCIPLE OF THE ASSAY<\/strong><\/span><\/h5>\n

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This immunogenicity assay measures the presence of human IgM in serum or plasma that bind to immobilized PEG using the direct ELISA technique. The supplied stretavidin precoated 96 well microplate is incubated with biotinylated PEG (5KDa). After washing, quality control and test samples are pipetted into the appropriate wells. Anti-PEG antibodies bind the immobilized PEG. After washing, detection antibody (anti human IgM Peroxidase) is added. Any unbound antibody enzyme reagent is removed with a final wash and a substrate solution is added to the wells for color development. Color development is proportional to the amount of anti-PEG IgM.<\/span><\/p>\n

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Product Specifications<\/span><\/strong><\/h3>\n

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    DETECTION METHOD : <\/span>Peroxidase \/ OD450<\/span><\/h6>\n

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    PRECISION : <\/span>Intra-assay coefficient of variation (CV) <10%. Inter-assay CV was <10%<\/span><\/h6>\n

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    DETECTION LIMIT : 62.5<\/span>ng\/ml<\/span><\/h6>\n

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    STORAGE CONDITIONS : <\/span>Stable at -20\u00b0C for 1 year<\/span><\/h6>\n

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    SAFETY WARNINGS AND PRECAUTIONS : <\/span>Research Use Only. Follow instructions.<\/span><\/h6>\n

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    EACH KIT INCLUDES :<\/span><\/h6>\n

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    – Coated microtiter plate<\/span>\u00a0<\/h6>\n
    – 96 wells QC samples : 6x250ul<\/span>\u00a0<\/h6>\n
    – 10X assay\/wash buffer : 50ml<\/span>\u00a0<\/h6>\n
    – 500X biotinylated PEG(5KDa) : 50ul<\/span>\u00a0<\/h6>\n
    – 1000X detection reagent : 17ul<\/span>\u00a0<\/h6>\n
    – TMB : 12ml<\/span>\u00a0<\/h6>\n
    – TMB stop solution : 12ml<\/span>\u00a0<\/h6>\n
    – Plate sealers : 3<\/span><\/h6>\n<\/div>\n<\/div>\n<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n

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    HOME Affinityimmuno ELISAs Anti PEG antibody ELISA (human IgM specific) CATALOG NUMBER :\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 EL-141-PEG-hIGM This immunogenicity assay measures the presence of human IgM in serum or plasma that bind to immobilized PEG using the direct ELISA technique. INTRODUCTION\u00a0Polyethylene glycol (PEG) chains are often used to modify therapeutic biologic agents in order […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"_links":{"self":[{"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=\/wp\/v2\/pages\/7515"}],"collection":[{"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=7515"}],"version-history":[{"count":4,"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=\/wp\/v2\/pages\/7515\/revisions"}],"predecessor-version":[{"id":7519,"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=\/wp\/v2\/pages\/7515\/revisions\/7519"}],"wp:attachment":[{"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=7515"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}