{"id":7495,"date":"2021-01-07T16:23:52","date_gmt":"2021-01-07T07:23:52","guid":{"rendered":"http:\/\/itsbio.co.kr\/?page_id=7495"},"modified":"2021-01-07T16:26:25","modified_gmt":"2021-01-07T07:26:25","slug":"trastuzumab-herceptin-immunogenicity-elisa","status":"publish","type":"page","link":"http:\/\/itsbio.co.kr\/?page_id=7495","title":{"rendered":"Trastuzumab (Herceptin\u00ae) Immunogenicity ELISA"},"content":{"rendered":"

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HOME<\/a> \"\" Affinityimmuno<\/a> \"\"ELISAs<\/a><\/p>\n<\/div>\n

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Trastuzumab (Herceptin\u00ae) Immunogenicity ELISA<\/strong><\/span><\/h1>\n<\/div>\n

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CATALOG NUMBER :\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 <\/span><\/span>EL-141-201<\/span><\/strong><\/p>\n

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Trastuzumab ELISA Herceptin ELISA immunogenicity Biosimilars<\/span><\/h3>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”3.25″ background_size=”initial” background_position=”top_left” background_repeat=”repeat”][et_pb_column type=”4_4″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.6.3″ vertical_offset_tablet=”0″ horizontal_offset_tablet=”0″ custom_margin=”||71px|||” z_index_tablet=”0″ text_text_shadow_horizontal_length_tablet=”0px” text_text_shadow_vertical_length_tablet=”0px” text_text_shadow_blur_strength_tablet=”1px” link_text_shadow_horizontal_length_tablet=”0px” link_text_shadow_vertical_length_tablet=”0px” link_text_shadow_blur_strength_tablet=”1px” ul_text_shadow_horizontal_length_tablet=”0px” ul_text_shadow_vertical_length_tablet=”0px” ul_text_shadow_blur_strength_tablet=”1px” ol_text_shadow_horizontal_length_tablet=”0px” ol_text_shadow_vertical_length_tablet=”0px” ol_text_shadow_blur_strength_tablet=”1px” quote_text_shadow_horizontal_length_tablet=”0px” quote_text_shadow_vertical_length_tablet=”0px” quote_text_shadow_blur_strength_tablet=”1px” header_text_shadow_horizontal_length_tablet=”0px” header_text_shadow_vertical_length_tablet=”0px” header_text_shadow_blur_strength_tablet=”1px” header_2_text_shadow_horizontal_length_tablet=”0px” header_2_text_shadow_vertical_length_tablet=”0px” header_2_text_shadow_blur_strength_tablet=”1px” header_3_text_shadow_horizontal_length_tablet=”0px” header_3_text_shadow_vertical_length_tablet=”0px” header_3_text_shadow_blur_strength_tablet=”1px” header_4_text_shadow_horizontal_length_tablet=”0px” header_4_text_shadow_vertical_length_tablet=”0px” header_4_text_shadow_blur_strength_tablet=”1px” header_5_text_shadow_horizontal_length_tablet=”0px” header_5_text_shadow_vertical_length_tablet=”0px” header_5_text_shadow_blur_strength_tablet=”1px” header_6_text_shadow_horizontal_length_tablet=”0px” header_6_text_shadow_vertical_length_tablet=”0px” header_6_text_shadow_blur_strength_tablet=”1px” box_shadow_horizontal_tablet=”0px” box_shadow_vertical_tablet=”0px” box_shadow_blur_tablet=”40px” box_shadow_spread_tablet=”0px”]<\/p>\n

INTRODUCTION<\/strong><\/span><\/h5>\n

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Trastuzumab (Herceptin\u00ae ) is a humanized recombinant monoclonal antibody used for the treatment of primary breast cancers overexpressing human epidermal growth factor 2 (HER2). Repeated dosing of Trastuzumab has been reported to induce a neutralizing antibody response resulting in reduce efficacy of Trastuzumab. This kit allows
researchers to qualitatively monitor the presence of\u00a0anti-Trastuzumab antibodies in biological matrices.<\/span><\/p>\n

\u00a0<\/p>\n

PRINCIPLE OF THE ASSAY<\/strong><\/span><\/h5>\n

\u00a0<\/p>\n

This immunogenicity assay employs the bridging ELISA technique. Capture antibody is precoated onto a 96 well microplate. Quality control and test samples are pipetted into the appropriate wells. Anti- Trastuzumab present in biological matrices is bound by the immobilized capture antibody. After washing away any unbound substances, secondary antibody is added to the wells and after a final wash a detection reagent is added. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development.
The color development is proportional to the amount\u00a0of anti-Trastuzumab present in test samples. Three levels of QC samples give a qualitative reference signal which can be used to determine the level of anti-Trastuzumab antibody in the unknown samples. The color development is stopped and the intensity of the color is measured.<\/span><\/p>\n

[\/et_pb_text][et_pb_text _builder_version=”4.6.3″ vertical_offset_tablet=”0″ horizontal_offset_tablet=”0″ z_index_tablet=”0″ text_text_shadow_horizontal_length_tablet=”0px” text_text_shadow_vertical_length_tablet=”0px” text_text_shadow_blur_strength_tablet=”1px” link_text_shadow_horizontal_length_tablet=”0px” link_text_shadow_vertical_length_tablet=”0px” link_text_shadow_blur_strength_tablet=”1px” ul_text_shadow_horizontal_length_tablet=”0px” ul_text_shadow_vertical_length_tablet=”0px” ul_text_shadow_blur_strength_tablet=”1px” ol_text_shadow_horizontal_length_tablet=”0px” ol_text_shadow_vertical_length_tablet=”0px” ol_text_shadow_blur_strength_tablet=”1px” quote_text_shadow_horizontal_length_tablet=”0px” quote_text_shadow_vertical_length_tablet=”0px” quote_text_shadow_blur_strength_tablet=”1px” header_text_shadow_horizontal_length_tablet=”0px” header_text_shadow_vertical_length_tablet=”0px” header_text_shadow_blur_strength_tablet=”1px” header_2_text_shadow_horizontal_length_tablet=”0px” header_2_text_shadow_vertical_length_tablet=”0px” header_2_text_shadow_blur_strength_tablet=”1px” header_3_text_shadow_horizontal_length_tablet=”0px” header_3_text_shadow_vertical_length_tablet=”0px” header_3_text_shadow_blur_strength_tablet=”1px” header_4_text_shadow_horizontal_length_tablet=”0px” header_4_text_shadow_vertical_length_tablet=”0px” header_4_text_shadow_blur_strength_tablet=”1px” header_5_text_shadow_horizontal_length_tablet=”0px” header_5_text_shadow_vertical_length_tablet=”0px” header_5_text_shadow_blur_strength_tablet=”1px” header_6_text_shadow_horizontal_length_tablet=”0px” header_6_text_shadow_vertical_length_tablet=”0px” header_6_text_shadow_blur_strength_tablet=”1px” box_shadow_horizontal_tablet=”0px” box_shadow_vertical_tablet=”0px” box_shadow_blur_tablet=”40px” box_shadow_spread_tablet=”0px”]<\/p>\n

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Product Specifications<\/span><\/strong><\/h3>\n

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    APPLICATION(S) : <\/span>measure anti-trastuzumab\u00a0antibodies<\/span><\/h6>\n

    \u00a0<\/p>\n<\/div>\n<\/div>\n<\/li>\n

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    TARGET PROTEIN : herceptin<\/span><\/span><\/h6>\n

    \u00a0<\/p>\n<\/div>\n<\/div>\n<\/li>\n

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    ALTERNATIVE NAME OF TARGET : trastuzumab<\/span><\/span><\/h6>\n

    \u00a0<\/p>\n<\/div>\n<\/div>\n<\/li>\n

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    \u00a0SIZE : <\/span>96 wells<\/span><\/h6>\n

    \u00a0<\/p>\n<\/div>\n<\/div>\n<\/li>\n

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    SENSITIVITY : <\/span>detection limit 1.5 ng\/mL<\/span><\/h6>\n

    \u00a0<\/p>\n<\/div>\n<\/div>\n<\/li>\n

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    DETECTION METHOD : <\/span>Peroxidase \/ OD450<\/span><\/h6>\n

    \u00a0<\/p>\n<\/div>\n<\/div>\n<\/li>\n

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    PRECISION : <\/span>Intra-assay coefficient of variation (CV) <10%. Inter-assay CV <10%<\/span><\/h6>\n

    \u00a0<\/p>\n<\/div>\n<\/div>\n<\/li>\n

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    DETECTION LIMIT : <\/span>31.3ng\/ml<\/span><\/h6>\n

    \u00a0<\/p>\n<\/div>\n<\/div>\n<\/li>\n

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    STORAGE CONDITIONS : <\/span>Stable at -20\u00b0C for 1 year<\/span><\/h6>\n

    \u00a0<\/p>\n<\/div>\n<\/div>\n<\/li>\n

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    SAFETY WARNINGS AND PRECAUTIONS : <\/span>Research Use Only. Follow instructions.<\/span><\/h6>\n

    \u00a0<\/p>\n<\/div>\n<\/div>\n<\/li>\n

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    EACH KIT INCLUDES :<\/span><\/h6>\n

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    – Coated microtiter plate<\/span>\u00a0<\/h6>\n
    – 96 wells QC samples : 4x50ul<\/span>\u00a0<\/h6>\n
    – 10X wash buffer : 25ml<\/span>\u00a0<\/h6>\n
    – Assay buffer : 50ml<\/span>\u00a0<\/h6>\n
    – 1000X secondary antibody\u00a0 : 17ul<\/span>\u00a0<\/h6>\n
    – 1000X detection reagent : 17ul<\/span>\u00a0<\/h6>\n
    – TMB : 12ml<\/span>\u00a0<\/h6>\n
    – TMB stop solution : 12ml<\/span>\u00a0<\/h6>\n
    – Plate sealers : 3<\/span><\/h6>\n<\/div>\n<\/div>\n<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n

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    HOME Affinityimmuno ELISAs Trastuzumab (Herceptin\u00ae) Immunogenicity ELISA CATALOG NUMBER :\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 EL-141-201 Trastuzumab ELISA Herceptin ELISA immunogenicity Biosimilars INTRODUCTION\u00a0Trastuzumab (Herceptin\u00ae ) is a humanized recombinant monoclonal antibody used for the treatment of primary breast cancers overexpressing human epidermal growth factor 2 (HER2). Repeated dosing of Trastuzumab has been reported to induce a […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"_links":{"self":[{"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=\/wp\/v2\/pages\/7495"}],"collection":[{"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=7495"}],"version-history":[{"count":3,"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=\/wp\/v2\/pages\/7495\/revisions"}],"predecessor-version":[{"id":7498,"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=\/wp\/v2\/pages\/7495\/revisions\/7498"}],"wp:attachment":[{"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=7495"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}