{"id":7479,"date":"2021-01-07T15:36:44","date_gmt":"2021-01-07T06:36:44","guid":{"rendered":"http:\/\/itsbio.co.kr\/?page_id=7479"},"modified":"2021-01-07T16:17:51","modified_gmt":"2021-01-07T07:17:51","slug":"tocilizumabactemra-immunogenicity-elisa-2","status":"publish","type":"page","link":"http:\/\/itsbio.co.kr\/?page_id=7479","title":{"rendered":"Tocilizumab(Actemra\u00ae) Immunogenicity ELISA"},"content":{"rendered":"

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HOME<\/a> \"\" Affinityimmuno<\/a> \"\"ELISAs<\/a><\/p>\n<\/div>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.4.2″ vertical_offset_tablet=”0″ horizontal_offset_tablet=”0″ z_index_tablet=”0″ custom_css_main_element=”padding-top:0;||padding-bottom:0;||marign-top:0;||marign-bottom:0;” box_shadow_horizontal_tablet=”0px” box_shadow_vertical_tablet=”0px” box_shadow_blur_tablet=”40px” box_shadow_spread_tablet=”0px”][et_pb_column type=”4_4″ _builder_version=”3.0.47″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_image src=”http:\/\/itsbio.co.kr\/wp-content\/uploads\/2021\/01\/affinityimmuno.png” _builder_version=”4.6.3″ vertical_offset_tablet=”0″ horizontal_offset_tablet=”0″ width=”25%” z_index_tablet=”0″ box_shadow_horizontal_tablet=”0px” box_shadow_vertical_tablet=”0px” box_shadow_blur_tablet=”40px” box_shadow_spread_tablet=”0px”][\/et_pb_image][et_pb_text _builder_version=”4.6.3″ vertical_offset_tablet=”0″ horizontal_offset_tablet=”0″ custom_padding=”||0px|||” hover_enabled=”0″ z_index_tablet=”0″ text_text_shadow_horizontal_length_tablet=”0px” text_text_shadow_vertical_length_tablet=”0px” text_text_shadow_blur_strength_tablet=”1px” link_text_shadow_horizontal_length_tablet=”0px” link_text_shadow_vertical_length_tablet=”0px” link_text_shadow_blur_strength_tablet=”1px” ul_text_shadow_horizontal_length_tablet=”0px” ul_text_shadow_vertical_length_tablet=”0px” ul_text_shadow_blur_strength_tablet=”1px” ol_text_shadow_horizontal_length_tablet=”0px” ol_text_shadow_vertical_length_tablet=”0px” ol_text_shadow_blur_strength_tablet=”1px” quote_text_shadow_horizontal_length_tablet=”0px” quote_text_shadow_vertical_length_tablet=”0px” quote_text_shadow_blur_strength_tablet=”1px” header_text_shadow_horizontal_length_tablet=”0px” header_text_shadow_vertical_length_tablet=”0px” header_text_shadow_blur_strength_tablet=”1px” header_2_text_shadow_horizontal_length_tablet=”0px” header_2_text_shadow_vertical_length_tablet=”0px” header_2_text_shadow_blur_strength_tablet=”1px” header_3_text_shadow_horizontal_length_tablet=”0px” header_3_text_shadow_vertical_length_tablet=”0px” header_3_text_shadow_blur_strength_tablet=”1px” header_4_text_shadow_horizontal_length_tablet=”0px” header_4_text_shadow_vertical_length_tablet=”0px” header_4_text_shadow_blur_strength_tablet=”1px” header_5_text_shadow_horizontal_length_tablet=”0px” header_5_text_shadow_vertical_length_tablet=”0px” header_5_text_shadow_blur_strength_tablet=”1px” header_6_text_shadow_horizontal_length_tablet=”0px” header_6_text_shadow_vertical_length_tablet=”0px” header_6_text_shadow_blur_strength_tablet=”1px” box_shadow_horizontal_tablet=”0px” box_shadow_vertical_tablet=”0px” box_shadow_blur_tablet=”40px” box_shadow_spread_tablet=”0px” sticky_enabled=”0″]<\/p>\n

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Tocilizumab(Actemra\u00ae) Immunogenicity ELISA<\/strong><\/span><\/h1>\n<\/div>\n

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CATALOG NUMBER :\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 <\/span><\/span>EL-141-202<\/span><\/strong><\/p>\n

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Tocilizumab (Actemra\u00ae ) is a humanized recombinant monoclonal antibody used for the treatment of inflammatory types of arthritis, such as rheumatoid arthritis (RA).<\/span><\/h3>\n

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INTRODUCTION<\/strong><\/span><\/h5>\n

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Tocilizumab (Actemra\u00ae ) is a humanized recombinant monoclonal antibody used for the treatment of inflammatory types of arthritis, such as rheumatoid arthritis (RA). Tocilizumab works by binding and blocking the activity of the IL6 receptor. This kit allows researchers to measure anti-tocilizumab antibodies in biological matrices.<\/span><\/p>\n

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PRINCIPLE OF THE ASSAY<\/strong><\/span><\/h5>\n

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This immunogenicity assay employs the bridging ELISA technique. Capture antibody (Tocilizumab) is precoated onto a 96 well microplate. Quality control and test samples are pipetted into the appropriate wells. Anti-tocilizumab present in biological matrices is bound by the immobilized capture antibody. After washing away any unbound substances, secondary antibody is added to the wells and after a final wash a detection reagent is added. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of anti-tocilizumab present in test samples. QC samples give a quantitative reference signal which can be<\/span>
used to determine the level of anti-tocilizumab antibody in the unknown samples. The color development is stopped and the intensity of the color is measured.<\/span><\/p>\n

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\"\"<\/span><\/p>\n

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6 replicates of each QC sample diluted 1 in 10 and <\/span>then used to generate a standard series ranging <\/span>from 500ng\/ml to 7.8ng\/ml anti-Tocilizumab. Assay <\/span>developed for 6 minutes in this example.<\/span><\/p>\n

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Product Specifications<\/span><\/strong><\/h3>\n

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    APPLICATION(S) : <\/span>measure anti-tocilizumab antibodies<\/span><\/h6>\n

    \u00a0<\/p>\n<\/div>\n<\/div>\n<\/li>\n

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    TARGET PROTEIN : <\/span>actemra<\/span><\/h6>\n

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    ALTERNATIVE NAME OF TARGET : <\/span>tocilizumab<\/span><\/h6>\n

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    \u00a0SIZE : <\/span>96 wells<\/span><\/h6>\n

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    SENSITIVITY : <\/span>detection limit 1.5 ng\/mL<\/span><\/h6>\n

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    DETECTION METHOD : <\/span>Peroxidase \/ OD450<\/span><\/h6>\n

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    PRECISION : <\/span>Intra-assay coefficient of variation (CV) <10%. Inter-assay CV was <10%<\/span><\/h6>\n

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    DETECTION LIMIT : <\/span>3.9ng\/ml<\/span><\/h6>\n

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    STORAGE CONDITIONS : <\/span>Stable at -20\u00b0C for 1 year<\/span><\/h6>\n

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    SAFETY WARNINGS AND PRECAUTIONS : <\/span>Research Use Only. Follow instructions.<\/span><\/h6>\n

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    EACH KIT INCLUDES :<\/span><\/h6>\n

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    – Coated microtiter plate<\/span>\u00a0<\/h6>\n
    – 96 wells QC samples : 8x50ul<\/span>\u00a0<\/h6>\n
    – 10X wash buffer : 50ml<\/span>\u00a0<\/h6>\n
    – Assay buffer : 50ml<\/span>\u00a0<\/h6>\n
    – 1000X secondary antibody\u00a0 : 17ul<\/span>\u00a0<\/h6>\n
    – 1000X detection reagent : 17ul<\/span>\u00a0<\/h6>\n
    – TMB : 12ml<\/span>\u00a0<\/h6>\n
    – TMB stop solution : 12ml<\/span>\u00a0<\/h6>\n
    – Plate sealers : 3<\/span><\/h6>\n<\/div>\n<\/div>\n<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n

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