{"id":6422,"date":"2020-09-28T11:32:54","date_gmt":"2020-09-28T02:32:54","guid":{"rendered":"http:\/\/itsbio.co.kr\/?page_id=6422"},"modified":"2020-09-30T11:21:40","modified_gmt":"2020-09-30T02:21:40","slug":"retrovirus-expression-systems","status":"publish","type":"page","link":"http:\/\/itsbio.co.kr\/?page_id=6422","title":{"rendered":"Retrovirus Expression Systems"},"content":{"rendered":"<p>[et_pb_section bb_built=&#8221;1&#8243; specialty=&#8221;off&#8221; _builder_version=&#8221;4.4.2&#8243; box_shadow_horizontal_tablet=&#8221;0px&#8221; box_shadow_vertical_tablet=&#8221;0px&#8221; box_shadow_blur_tablet=&#8221;40px&#8221; box_shadow_spread_tablet=&#8221;0px&#8221; custom_css_main_element=&#8221;padding:0;||margin:0;&#8221; vertical_offset_tablet=&#8221;0&#8243; horizontal_offset_tablet=&#8221;0&#8243; z_index_tablet=&#8221;0&#8243; next_background_color=&#8221;#000000&#8243;][et_pb_row _builder_version=&#8221;4.4.2&#8243; box_shadow_horizontal_tablet=&#8221;0px&#8221; box_shadow_vertical_tablet=&#8221;0px&#8221; 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header_4_text_shadow_horizontal_length=&#8221;header_4_text_shadow_style,%91object Object%93&#8243; header_4_text_shadow_horizontal_length_tablet=&#8221;0px&#8221; header_4_text_shadow_vertical_length=&#8221;header_4_text_shadow_style,%91object Object%93&#8243; header_4_text_shadow_vertical_length_tablet=&#8221;0px&#8221; header_4_text_shadow_blur_strength=&#8221;header_4_text_shadow_style,%91object Object%93&#8243; header_4_text_shadow_blur_strength_tablet=&#8221;1px&#8221; header_5_text_shadow_horizontal_length=&#8221;header_5_text_shadow_style,%91object Object%93&#8243; header_5_text_shadow_horizontal_length_tablet=&#8221;0px&#8221; header_5_text_shadow_vertical_length=&#8221;header_5_text_shadow_style,%91object Object%93&#8243; header_5_text_shadow_vertical_length_tablet=&#8221;0px&#8221; header_5_text_shadow_blur_strength=&#8221;header_5_text_shadow_style,%91object Object%93&#8243; header_5_text_shadow_blur_strength_tablet=&#8221;1px&#8221; header_6_text_shadow_horizontal_length=&#8221;header_6_text_shadow_style,%91object Object%93&#8243; header_6_text_shadow_horizontal_length_tablet=&#8221;0px&#8221; header_6_text_shadow_vertical_length=&#8221;header_6_text_shadow_style,%91object Object%93&#8243; header_6_text_shadow_vertical_length_tablet=&#8221;0px&#8221; header_6_text_shadow_blur_strength=&#8221;header_6_text_shadow_style,%91object Object%93&#8243; header_6_text_shadow_blur_strength_tablet=&#8221;1px&#8221; box_shadow_horizontal_tablet=&#8221;0px&#8221; box_shadow_vertical_tablet=&#8221;0px&#8221; box_shadow_blur_tablet=&#8221;40px&#8221; box_shadow_spread_tablet=&#8221;0px&#8221; vertical_offset_tablet=&#8221;0&#8243; horizontal_offset_tablet=&#8221;0&#8243; z_index_tablet=&#8221;0&#8243;]<\/p>\n<div class=\"overview\">\n<div class=\"text_area01\">\n<p class=\"text\">Retroviral vectors have proven to be useful tools for either\u00a0<i>in vitro<\/i>\u00a0or\u00a0<i>in vivo<\/i>\u00a0stable integration of a transduced gene into the host cell chromosome via the integration mechanisms that they normally employ during their normal life cycle.<\/p>\n<p>&nbsp;<\/p>\n<p>One of the significant advances in retroviral expression systems in recent years is the production of high titer recombinant retrovirus by transient transfection. The improvement allows for the production of high titer retroviruses within a week and eliminates the time-consuming process of generating a viral producer cell line.<\/p>\n<p>&nbsp;<\/p>\n<p>Our Retro-Easy\u2122 retroviral expression system includes a comprehensive line of retroviral vectors with very flexible multiple cloning sites for easy subcloning of your gene of interest into any of our retroviral vectors. The His and HA fusion tag compatible vectors (pRetro-E1\u2122-HA and pRetro-E2\u2122-His) allow for fusion gene expression in retroviral vectors with either a His or HA tag.<\/p>\n<p>&nbsp;<\/p>\n<p>All our retroviral vectors are derived from Moloney Murine Leukemia Virus (MoMuLV).<\/p>\n<p>&nbsp;<\/p>\n<p>Need help constructing your retrovirus? Check out our\u00a0<a class=\"orange-link\" href=\"\/?page_id=165\" target=\"_blank\" rel=\"noopener noreferrer\">Contact<\/a>.<\/p>\n<p>&nbsp;<\/p>\n<\/div>\n<p><span class=\"bluetext\"><b>Popular Products<\/b><\/span><\/p>\n<ul class=\"grey-list grey-link\">\n<li><a class=\"abm-category-list-cname\" href=\"https:\/\/www.abmgood.com\/retro-easy-his-e-519.html\" target=\"_blank\" rel=\"noopener noreferrer\">Retro-Easy-His Cloning Kit<\/a><\/li>\n<li><a class=\"abm-category-list-cname\" href=\"https:\/\/www.abmgood.com\/pretrog-cmv-gfp-ires-gfp-vector-rv004.html\" target=\"_blank\" rel=\"noopener noreferrer\">pRetroG-CMV-GFP-IRES-GFP Vector<\/a><\/li>\n<li><a class=\"abm-category-list-cname\" href=\"https:\/\/www.abmgood.com\/retro-easy1-e-512.html\" target=\"_blank\" rel=\"noopener noreferrer\">Retro-Easy1 Cloning Kit<\/a><\/li>\n<li><a class=\"abm-category-list-cname\" href=\"https:\/\/www.abmgood.com\/retro-easy2-e-513.html\" target=\"_blank\" rel=\"noopener noreferrer\">Retro-Easy2 Cloning Kit<\/a><\/li>\n<li><a class=\"abm-category-list-cname\" href=\"\/?page_id=6435\" target=\"_blank\" rel=\"noopener noreferrer\">Retrovirus Packaging Systems<\/a><\/li>\n<\/ul>\n<\/div>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section bb_built=&#8221;1&#8243; 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horizontal_offset_tablet=&#8221;0&#8243; z_index_tablet=&#8221;0&#8243;]<\/p>\n<ul class=\"abm-document-list\" data-count=\"1\">\n<li><img decoding=\"async\" src=\"http:\/\/itsbio.co.kr\/wp-content\/uploads\/2020\/07\/pdf-log.png\" \/>\u00a0<a class=\"black-link doclinks\" href=\"https:\/\/www.abmgood.com\/pub\/media\/catalog\/category\/docs\/r\/e\/Retrovirus-Risk-Assessment.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Retrovirus Risk Assessment<\/a><\/li>\n<li><img decoding=\"async\" src=\"http:\/\/itsbio.co.kr\/wp-content\/uploads\/2020\/07\/pdf-log.png\" \/>\u00a0<a class=\"black-link doclinks\" href=\"https:\/\/www.abmgood.com\/pub\/media\/catalog\/category\/docs\/r\/e\/Retrovirus-Expression-Protocol_edit_dec_14.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Retroviral Vector User Manual<\/a><\/li>\n<li><img decoding=\"async\" src=\"http:\/\/itsbio.co.kr\/wp-content\/uploads\/2020\/07\/pdf-log.png\" \/>\u00a0<a class=\"black-link doclinks\" href=\"https:\/\/www.abmgood.com\/pub\/media\/catalog\/category\/docs\/v\/i\/Viral-Vectors-Brochure-Single-Pages.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Viral Vector Brochure<\/a><\/li>\n<\/ul>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section bb_built=&#8221;1&#8243; 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horizontal_offset_tablet=&#8221;0&#8243; z_index_tablet=&#8221;0&#8243;]<\/p>\n<table width=\"747\">\n<tbody>\n<tr>\n<td colspan=\"2\" width=\"747\">\n<h3><span style=\"color: #000000;\"><strong>Retroviral Vector<\/strong><\/span><\/h3>\n<\/td>\n<\/tr>\n<tr>\n<td>RV001<\/td>\n<td><a href=\"https:\/\/www.abmgood.com\/pretrog-cmv-blank-vector-rv001.html\" target=\"blank\" rel=\"noopener noreferrer\">pRetroG-CMV-blank Vector<\/a><\/td>\n<\/tr>\n<tr>\n<td>RV002<\/td>\n<td><a href=\"https:\/\/www.abmgood.com\/pretrog-cmv-gfp-ha-vector-rv002.html\" target=\"blank\" rel=\"noopener noreferrer\">pRetroG-CMV-GFP-HA Vector<\/a><\/td>\n<\/tr>\n<tr>\n<td>RV003<\/td>\n<td><a href=\"https:\/\/www.abmgood.com\/pretrog-cmv-gfp-stop-vector-rv003.html\" target=\"blank\" rel=\"noopener noreferrer\">pRetroG-CMV-GFP-stop Vector<\/a><\/td>\n<\/tr>\n<tr>\n<td>RV004<\/td>\n<td><a href=\"https:\/\/www.abmgood.com\/pretrog-cmv-gfp-ires-gfp-vector-rv004.html\" target=\"blank\" rel=\"noopener noreferrer\">pRetroG-CMV-GFP-IRES-GFP Vector<\/a><\/td>\n<\/tr>\n<tr>\n<td>RV005<\/td>\n<td><a href=\"https:\/\/www.abmgood.com\/pretrog-cmv-rfp-stop-vector-rv005.html\" target=\"blank\" rel=\"noopener noreferrer\">pRetroG-CMV-RFP-stop Vector<\/a><\/td>\n<\/tr>\n<tr>\n<td>\u00a0<\/td>\n<td>\u00a0<\/td>\n<\/tr>\n<tr>\n<td colspan=\"2\"><span style=\"color: #000000;\"><strong>Retrovirus<\/strong><\/span><\/td>\n<\/tr>\n<tr>\n<td>RVP001<\/td>\n<td><a href=\"https:\/\/www.abmgood.com\/retro-cmv-blank-retrovirus-rvp001.html\" target=\"blank\" rel=\"noopener noreferrer\">Retro-CMV Blank Retrovirus<\/a><\/td>\n<\/tr>\n<tr>\n<td>RVP002<\/td>\n<td><a href=\"https:\/\/www.abmgood.com\/retro-cmv-gfp-ha-retrovirus-rvp002.html\" target=\"blank\" rel=\"noopener noreferrer\">Retro-CMV-GFP-HA Retrovirus<\/a><\/td>\n<\/tr>\n<tr>\n<td>RVP003<\/td>\n<td><a href=\"https:\/\/www.abmgood.com\/retro-cmv-gfp-retrovirus-rvp003.html\" target=\"blank\" rel=\"noopener noreferrer\">Retro-CMV-GFP Retrovirus<\/a><\/td>\n<\/tr>\n<tr>\n<td>RVP004<\/td>\n<td><a href=\"https:\/\/www.abmgood.com\/retro-cmv-gfp-ires-gfp-retrovirus-rvp004.html\" target=\"blank\" rel=\"noopener noreferrer\">Retro-CMV-GFP-IRES-GFP Retrovirus<\/a><\/td>\n<\/tr>\n<tr>\n<td>RVP005<\/td>\n<td><a href=\"https:\/\/www.abmgood.com\/retro-cmv-rfp-retrovirus-rvp005.html\" target=\"blank\" rel=\"noopener noreferrer\">Retro-CMV-RFP Retrovirus<\/a><\/td>\n<\/tr>\n<tr>\n<td>\u00a0<\/td>\n<td>\u00a0<\/td>\n<\/tr>\n<tr>\n<td>\u00a0<\/td>\n<td>\u00a0<\/td>\n<\/tr>\n<tr>\n<td colspan=\"2\"><span style=\"color: #000000;\"><strong>5&#8217;LTR\u00a0<\/strong><\/span><\/td>\n<\/tr>\n<tr>\n<td>E-512<\/td>\n<td><a href=\"https:\/\/www.abmgood.com\/retro-easy1-e-512.html\" target=\"blank\" rel=\"noopener noreferrer\">Retro-Easy1 Cloning Kit<\/a><\/td>\n<\/tr>\n<tr>\n<td>E-514<\/td>\n<td><a href=\"https:\/\/www.abmgood.com\/pretro-e1-e-514.html\" target=\"blank\" rel=\"noopener noreferrer\">pRetro-E1<\/a><\/td>\n<\/tr>\n<tr>\n<td>E-516<\/td>\n<td><a href=\"https:\/\/www.abmgood.com\/pretro-e1-ha-e-516.html\" target=\"blank\" rel=\"noopener noreferrer\">pRetro-E1-HA<\/a><\/td>\n<\/tr>\n<tr>\n<td>E-518<\/td>\n<td><a href=\"https:\/\/www.abmgood.com\/retro-easy-ha-e-518.html\" target=\"blank\" rel=\"noopener noreferrer\">Retro-Easy-HA Cloning Kit<\/a><\/td>\n<\/tr>\n<tr>\n<td>\u00a0<\/td>\n<td>\u00a0<\/td>\n<\/tr>\n<tr>\n<td colspan=\"2\"><span style=\"color: #000000;\"><strong>CMV<\/strong><\/span><\/td>\n<\/tr>\n<tr>\n<td>E-513<\/td>\n<td><a href=\"https:\/\/www.abmgood.com\/retro-easy2-e-513.html\" target=\"blank\" rel=\"noopener noreferrer\">Retro-Easy2 Cloning Kit<\/a><\/td>\n<\/tr>\n<tr>\n<td>E-515<\/td>\n<td><a href=\"https:\/\/www.abmgood.com\/pretro-e2-e-515.html\" target=\"blank\" rel=\"noopener noreferrer\">pRetro-E2<\/a><\/td>\n<\/tr>\n<tr>\n<td>E-517<\/td>\n<td><a href=\"https:\/\/www.abmgood.com\/pretro-e2-his-e-517.html\" target=\"blank\" rel=\"noopener noreferrer\">pRetro-E2-His<\/a><\/td>\n<\/tr>\n<tr>\n<td>E-519<\/td>\n<td><a href=\"https:\/\/www.abmgood.com\/retro-easy-his-e-519.html\" target=\"blank\" rel=\"noopener noreferrer\">Retro-Easy-His Cloning Kit<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section bb_built=&#8221;1&#8243; 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One of the significant advances [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"_links":{"self":[{"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=\/wp\/v2\/pages\/6422"}],"collection":[{"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=6422"}],"version-history":[{"count":7,"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=\/wp\/v2\/pages\/6422\/revisions"}],"predecessor-version":[{"id":8042,"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=\/wp\/v2\/pages\/6422\/revisions\/8042"}],"wp:attachment":[{"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=6422"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}