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HOME<\/a> [\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.16″ vertical_offset_tablet=”0″ horizontal_offset_tablet=”0″ custom_margin=”||-80px||false|false” z_index_tablet=”0″ custom_css_main_element=”padding-top:0;” box_shadow_horizontal_tablet=”0px” box_shadow_vertical_tablet=”0px” box_shadow_blur_tablet=”40px” box_shadow_spread_tablet=”0px” global_colors_info=”{}”][et_pb_row column_structure=”2_3,1_3″ _builder_version=”4.16″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” global_colors_info=”{}”][et_pb_column type=”2_3″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.16″ vertical_offset_tablet=”0″ horizontal_offset_tablet=”0″ custom_margin=”||-80px||false|false” z_index_tablet=”0″ text_text_shadow_horizontal_length_tablet=”0px” text_text_shadow_vertical_length_tablet=”0px” text_text_shadow_blur_strength_tablet=”1px” link_text_shadow_horizontal_length_tablet=”0px” link_text_shadow_vertical_length_tablet=”0px” link_text_shadow_blur_strength_tablet=”1px” ul_text_shadow_horizontal_length_tablet=”0px” ul_text_shadow_vertical_length_tablet=”0px” ul_text_shadow_blur_strength_tablet=”1px” ol_text_shadow_horizontal_length_tablet=”0px” ol_text_shadow_vertical_length_tablet=”0px” ol_text_shadow_blur_strength_tablet=”1px” quote_text_shadow_horizontal_length_tablet=”0px” quote_text_shadow_vertical_length_tablet=”0px” quote_text_shadow_blur_strength_tablet=”1px” header_text_shadow_horizontal_length_tablet=”0px” header_text_shadow_vertical_length_tablet=”0px” header_text_shadow_blur_strength_tablet=”1px” header_2_text_shadow_horizontal_length_tablet=”0px” header_2_text_shadow_vertical_length_tablet=”0px” header_2_text_shadow_blur_strength_tablet=”1px” header_3_text_shadow_horizontal_length_tablet=”0px” header_3_text_shadow_vertical_length_tablet=”0px” header_3_text_shadow_blur_strength_tablet=”1px” header_4_text_shadow_horizontal_length_tablet=”0px” header_4_text_shadow_vertical_length_tablet=”0px” header_4_text_shadow_blur_strength_tablet=”1px” header_5_text_shadow_horizontal_length_tablet=”0px” header_5_text_shadow_vertical_length_tablet=”0px” header_5_text_shadow_blur_strength_tablet=”1px” header_6_text_shadow_horizontal_length_tablet=”0px” header_6_text_shadow_vertical_length_tablet=”0px” header_6_text_shadow_blur_strength_tablet=”1px” box_shadow_horizontal_tablet=”0px” box_shadow_vertical_tablet=”0px” box_shadow_blur_tablet=”40px” box_shadow_spread_tablet=”0px” global_colors_info=”{}”]<\/p>\n The most advanced RNA tracking, visualization and pull down technology.<\/strong> <\/p>\n Key Features<\/strong><\/p>\n <\/p>\n RNA Mango technology is based on the specific binding of the RNA Mango Aptamer and a Thizole Orange (TO) bi-functional dye. The main features of this technology is the tight binding between the dye and aptamer (KD \u2248 3nM) , and the strong ~1000X enhancement of the dye\u2019s fluorescence when bound to the Mango aptamer (Fluorescent enhancement FE=1,100).<\/p>\n <\/p>\n The Thizole Orange (TO) dye has a number of other desirable properties including:<\/p>\n <\/p>\n TO1-biotin is the standard variety of TO dye for RNA Mango experiments.\u00a0View<\/span><\/a>\u00a0our complete list of RNA Mango dyes.<\/p>\n Can’t find what you’re looking for, or have a specific request? <\/p>\n <\/p>\n [\/et_pb_text][\/et_pb_column][et_pb_column type=”1_3″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.16″ _module_preset=”default” background_color=”#ffffff” global_colors_info=”{}”]<\/p>\n Popular Products<\/b><\/span><\/p>\n Documents<\/b><\/span><\/p>\n [\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ 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header_text_shadow_blur_strength_tablet=”1px” header_2_text_shadow_horizontal_length_tablet=”0px” header_2_text_shadow_vertical_length_tablet=”0px” header_2_text_shadow_blur_strength_tablet=”1px” header_3_text_shadow_horizontal_length_tablet=”0px” header_3_text_shadow_vertical_length_tablet=”0px” header_3_text_shadow_blur_strength_tablet=”1px” header_4_text_shadow_horizontal_length_tablet=”0px” header_4_text_shadow_vertical_length_tablet=”0px” header_4_text_shadow_blur_strength_tablet=”1px” header_5_text_shadow_horizontal_length_tablet=”0px” header_5_text_shadow_vertical_length_tablet=”0px” header_5_text_shadow_blur_strength_tablet=”1px” header_6_text_shadow_horizontal_length_tablet=”0px” header_6_text_shadow_vertical_length_tablet=”0px” header_6_text_shadow_blur_strength_tablet=”1px” box_shadow_horizontal_tablet=”0px” box_shadow_vertical_tablet=”0px” box_shadow_blur_tablet=”40px” box_shadow_spread_tablet=”0px” global_colors_info=”{}”]<\/p>\n Laboratory Results<\/p>\n<\/div>\n [\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_2,1_2″ _builder_version=”4.16″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” global_colors_info=”{}”][et_pb_column type=”1_2″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.16″ vertical_offset_tablet=”0″ horizontal_offset_tablet=”0″ z_index_tablet=”0″ text_text_shadow_horizontal_length_tablet=”0px” text_text_shadow_vertical_length_tablet=”0px” text_text_shadow_blur_strength_tablet=”1px” link_text_shadow_horizontal_length_tablet=”0px” link_text_shadow_vertical_length_tablet=”0px” link_text_shadow_blur_strength_tablet=”1px” ul_text_shadow_horizontal_length_tablet=”0px” ul_text_shadow_vertical_length_tablet=”0px” ul_text_shadow_blur_strength_tablet=”1px” ol_text_shadow_horizontal_length_tablet=”0px” ol_text_shadow_vertical_length_tablet=”0px” ol_text_shadow_blur_strength_tablet=”1px” 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header_6_text_shadow_vertical_length_tablet=”0px” header_6_text_shadow_blur_strength_tablet=”1px” box_shadow_horizontal_tablet=”0px” box_shadow_vertical_tablet=”0px” box_shadow_blur_tablet=”40px” box_shadow_spread_tablet=”0px” global_colors_info=”{}”]<\/p>\n \n [\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_2,1_2″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”1_2″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”]<\/p>\n![\"\"](\"http:\/\/itsbio.kjhhkh.gethompy.com\/wp-content\/uploads\/2020\/04\/arrow.png\"){kind=icon}
abm<\/a> Molecular Biology and PCR<\/a> RNA Tracking & Visualization (RNA Mango)
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Technology for studying the diverse cellular roles of RNA has lagged behind the tools for studying DNA and proteins, but innovative researchers are working to change that! One such researcher is\u00a0Dr. Peter Unrau of Simon Fraser University<\/a>.<\/span> He and his team have created RNA Mango, a novel technology with a number of useful applications.<\/p>\n\n
Speak to one of our technical support specialists today\u00a0technical@abmgood.com<\/a>.<\/span><\/p>\n\n
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![\"The](\"http:\/\/itsbio.co.kr\/wp-content\/uploads\/2020\/09\/RNA-1.jpg\")
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The system has two components: the RNA Mango aptamer, and the TO-1 dye. The dye only fluoresces when bound to the Mango aptamer.<\/div>\n![\"Tagging](\"http:\/\/itsbio.co.kr\/wp-content\/uploads\/2020\/09\/RNA-2.jpg\")
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For mRNA inset the tag into the 3’UTR. For structured non-coding RNA replace a stem-loop that is not essential to the RNA function.<\/div>\n![\"Application:](\"http:\/\/itsbio.co.kr\/wp-content\/uploads\/2020\/09\/RNA-3.jpg\")
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Express RNA of interest (with Mango Tag) using a vector or CRISPR Knock-In. Soak cells in TO-1 Biotin dye to illuminate RNA of interest localization<\/div>\n![\"pApplication:](\"http:\/\/itsbio.co.kr\/wp-content\/uploads\/2020\/09\/RNA-4.jpg\")
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Express RNA of interest (with Mango Tag) using a vector or CRISPR Knock-In. Lyse the cells, and recover RNA of interest with bound RNA or proteins using streptavidin breads<\/div>\n![\"Yeast](\"http:\/\/itsbio.co.kr\/wp-content\/uploads\/2020\/09\/RNA-Mango_RNP-Pulldown-Purification_data-01.png\")
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Left: RNAs present in native extract in which U1M migrates as doublet. Right: U1M is a single band RNP present after mango-based purification using TO1 3PEG-Desthiobiotin Fluorophore<\/div>\n![\"RNA](\"http:\/\/itsbio.co.kr\/wp-content\/uploads\/2020\/09\/magno.png\")
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Mango dye binding to in vitro<\/i> transcribed RNA Mango-tagged sgRNA. Stable binding and fluorescence even after leaving the tube for 1 month at room temperature.<\/div>\n\n