{"id":5221,"date":"2020-08-13T12:30:23","date_gmt":"2020-08-13T03:30:23","guid":{"rendered":"http:\/\/itsbio.co.kr\/?page_id=5221"},"modified":"2020-08-13T12:30:23","modified_gmt":"2020-08-13T03:30:23","slug":"comet-assay-tanks","status":"publish","type":"page","link":"http:\/\/itsbio.co.kr\/?page_id=5221","title":{"rendered":"Comet Assay Tanks"},"content":{"rendered":"<p>[et_pb_section bb_built=&#8221;1&#8243; specialty=&#8221;off&#8221; _builder_version=&#8221;4.4.2&#8243; box_shadow_horizontal_tablet=&#8221;0px&#8221; box_shadow_vertical_tablet=&#8221;0px&#8221; box_shadow_blur_tablet=&#8221;40px&#8221; box_shadow_spread_tablet=&#8221;0px&#8221; custom_css_main_element=&#8221;padding:0;||margin:0;&#8221; vertical_offset_tablet=&#8221;0&#8243; horizontal_offset_tablet=&#8221;0&#8243; z_index_tablet=&#8221;0&#8243; next_background_color=&#8221;#000000&#8243;][et_pb_row _builder_version=&#8221;4.4.2&#8243; box_shadow_horizontal_tablet=&#8221;0px&#8221; box_shadow_vertical_tablet=&#8221;0px&#8221; 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box_shadow_vertical_tablet=&#8221;0px&#8221; box_shadow_blur_tablet=&#8221;40px&#8221; box_shadow_spread_tablet=&#8221;0px&#8221; vertical_offset_tablet=&#8221;0&#8243; horizontal_offset_tablet=&#8221;0&#8243; z_index_tablet=&#8221;0&#8243;]<\/p>\n<h2><strong>Description<\/strong><\/h2>\n<p>\u00a0<\/p>\n<p>Cleaver Scientific Comet Assay Tanks are available in four slide formats to study single cell gel electrophoresis (SCGE), a technique made popular by drug toxicology and carcinogenesis studies for the detection and quantification of DNA damage in cells. Each tank\u2019s robust construction from ebony acrylic ensures that cells remain free of exposure to background light and DNA damage during electrophoresis, while a cooled central platform provides a convenient surface for slide preparation and control of slide temperature during the assay. Following electrophoresis DNA damage may be measured using Comet Assay scoring software.<\/p>\n<p>\u00a0<\/p>\n<p><b>Background<\/b>: First introduced in 1981 to quantify double-stranded DNA breakages in single cells exposed to irradiation, the Comet Assay (or SCGE) has since been adapted to analyse specific DNA lesions and repair processes.<\/p>\n<p>\u00a0<\/p>\n<p><b>Overview<\/b>: Following genotoxic insult, such as ionizing radiation, the resultant strand breakage of supercoiled duplex DNA reduces the size of the large genomic DNA from which these strands are separated or drawn out by electrophoresis. The genomic DNA then takes on the appearance of a comet as its negatively charged broken ends and fragments migrate towards the anode during electrophoresis.<\/p>\n<p>\u00a0<\/p>\n<p><b>Method<\/b>: After exposure to a genotoxic insult cells are suspended within low melting point agarose and embedded within a thin layer of agarose on a microscope slide. Cellular protein is then removed by lysis in detergent, when DNA is allowed to unwind in alkaline conditions before electrophoresis. The DNA is electrophoresed, stained and then analysed using software.<\/p>\n<p>\u00a0<\/p>\n<p><b>Results<\/b>: Microscopy imaging is used to measure DNA fluorescence upon staining. In DNA damaged cells the resultant image resembles a \u2018comet\u2019 with the cellular DNA separated into a head and tail. The head is mainly composed of intact genomic DNA, whereas any fragmented or damaged DNA is concentrated within and towards the tail.<\/p>\n<p>\u00a0<\/p>\n<p>Catalogue Page Number: 50<\/p>\n<p>This unit has a 12 month limited warranty, for more information, consult our terms and conditions.<\/p>\n<p>\u00a0<\/p>\n<p>\u00a0<\/p>\n<p>[\/et_pb_text][et_pb_text _builder_version=&#8221;4.5.3&#8243; text_text_shadow_horizontal_length=&#8221;text_text_shadow_style,%91object Object%93&#8243; text_text_shadow_horizontal_length_tablet=&#8221;0px&#8221; text_text_shadow_vertical_length=&#8221;text_text_shadow_style,%91object Object%93&#8243; text_text_shadow_vertical_length_tablet=&#8221;0px&#8221; text_text_shadow_blur_strength=&#8221;text_text_shadow_style,%91object Object%93&#8243; text_text_shadow_blur_strength_tablet=&#8221;1px&#8221; link_text_shadow_horizontal_length=&#8221;link_text_shadow_style,%91object Object%93&#8243; link_text_shadow_horizontal_length_tablet=&#8221;0px&#8221; link_text_shadow_vertical_length=&#8221;link_text_shadow_style,%91object Object%93&#8243; 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header_6_text_shadow_vertical_length_tablet=&#8221;0px&#8221; header_6_text_shadow_blur_strength=&#8221;header_6_text_shadow_style,%91object Object%93&#8243; header_6_text_shadow_blur_strength_tablet=&#8221;1px&#8221; box_shadow_horizontal_tablet=&#8221;0px&#8221; box_shadow_vertical_tablet=&#8221;0px&#8221; box_shadow_blur_tablet=&#8221;40px&#8221; box_shadow_spread_tablet=&#8221;0px&#8221; vertical_offset_tablet=&#8221;0&#8243; horizontal_offset_tablet=&#8221;0&#8243; z_index_tablet=&#8221;0&#8243;]<\/p>\n<h2><strong>Technical Specifications<\/strong><\/h2>\n<p>\u00a0<\/p>\n<table>\n<tbody>\n<tr>\n<th>SKU<\/th>\n<th>CSL-COM10<\/th>\n<th>CSL-COM20<\/th>\n<th>CSL-COM40<\/th>\n<\/tr>\n<tr>\n<th>Unit Dimensions (cm)<\/th>\n<td>17 x 34 x 9<\/td>\n<td>31 x 34 x 9<\/td>\n<td>33 x 59 x 9<\/td>\n<\/tr>\n<tr>\n<th>Slide Capacity<\/th>\n<td>10<\/td>\n<td>20<\/td>\n<td>40<\/td>\n<\/tr>\n<tr>\n<th>Base Buffer Volume<\/th>\n<td>550<\/td>\n<td>1000<\/td>\n<td>2100<\/td>\n<\/tr>\n<tr>\n<th>Typical Running Conditions<\/th>\n<td>25V\/300mA for 1h<\/td>\n<td>25V\/300mA for 1h<\/td>\n<td>25V\/300mA for 1h<\/td>\n<\/tr>\n<tr>\n<th>Includes Casting Base<\/th>\n<td>FALSE<\/td>\n<td>FALSE<\/td>\n<td>FALSE<\/td>\n<\/tr>\n<tr>\n<th>Includes Additional Caster<\/th>\n<td>FALSE<\/td>\n<td>FALSE<\/td>\n<td>FALSE<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>\u00a0<\/p>\n<p>\u00a0<\/p>\n<p>[\/et_pb_text][et_pb_text _builder_version=&#8221;4.5.3&#8243; 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