{"id":18858,"date":"2024-12-20T09:56:06","date_gmt":"2024-12-20T00:56:06","guid":{"rendered":"http:\/\/itsbio.co.kr\/?page_id=18858"},"modified":"2024-12-20T09:56:09","modified_gmt":"2024-12-20T00:56:09","slug":"gcsf-ada-granulocyte-colony-stimulating-factor-elisa-el-141-73196","status":"publish","type":"page","link":"http:\/\/itsbio.co.kr\/?page_id=18858","title":{"rendered":"GCSF ADA (granulocyte colony stimulating factor) ELISA | EL-141-73196"},"content":{"rendered":"

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HOME<\/a> \"\" Affinityimmuno<\/a> \"\"ELISAs<\/a><\/p>\n<\/div>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.16″ vertical_offset_tablet=”0″ horizontal_offset_tablet=”0″ z_index_tablet=”0″ custom_css_main_element=”padding-top:0;||padding-bottom:0;||marign-top:0;||marign-bottom:0;” box_shadow_horizontal_tablet=”0px” box_shadow_vertical_tablet=”0px” box_shadow_blur_tablet=”40px” box_shadow_spread_tablet=”0px” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_column type=”4_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||” theme_builder_area=”post_content”][et_pb_image src=”http:\/\/itsbio.co.kr\/wp-content\/uploads\/2021\/01\/affinityimmuno.png” _builder_version=”4.16″ vertical_offset_tablet=”0″ horizontal_offset_tablet=”0″ width=”25%” z_index_tablet=”0″ box_shadow_horizontal_tablet=”0px” box_shadow_vertical_tablet=”0px” box_shadow_blur_tablet=”40px” box_shadow_spread_tablet=”0px” global_colors_info=”{}” theme_builder_area=”post_content”][\/et_pb_image][et_pb_text _builder_version=”4.23″ vertical_offset_tablet=”0″ horizontal_offset_tablet=”0″ custom_padding=”||0px|||” hover_enabled=”0″ z_index_tablet=”0″ text_text_shadow_horizontal_length_tablet=”0px” text_text_shadow_vertical_length_tablet=”0px” text_text_shadow_blur_strength_tablet=”1px” link_text_shadow_horizontal_length_tablet=”0px” link_text_shadow_vertical_length_tablet=”0px” link_text_shadow_blur_strength_tablet=”1px” ul_text_shadow_horizontal_length_tablet=”0px” ul_text_shadow_vertical_length_tablet=”0px” ul_text_shadow_blur_strength_tablet=”1px” ol_text_shadow_horizontal_length_tablet=”0px” ol_text_shadow_vertical_length_tablet=”0px” ol_text_shadow_blur_strength_tablet=”1px” quote_text_shadow_horizontal_length_tablet=”0px” quote_text_shadow_vertical_length_tablet=”0px” quote_text_shadow_blur_strength_tablet=”1px” header_text_shadow_horizontal_length_tablet=”0px” header_text_shadow_vertical_length_tablet=”0px” header_text_shadow_blur_strength_tablet=”1px” header_2_text_shadow_horizontal_length_tablet=”0px” header_2_text_shadow_vertical_length_tablet=”0px” header_2_text_shadow_blur_strength_tablet=”1px” header_3_text_shadow_horizontal_length_tablet=”0px” header_3_text_shadow_vertical_length_tablet=”0px” header_3_text_shadow_blur_strength_tablet=”1px” header_4_text_shadow_horizontal_length_tablet=”0px” header_4_text_shadow_vertical_length_tablet=”0px” header_4_text_shadow_blur_strength_tablet=”1px” header_5_text_shadow_horizontal_length_tablet=”0px” header_5_text_shadow_vertical_length_tablet=”0px” header_5_text_shadow_blur_strength_tablet=”1px” header_6_text_shadow_horizontal_length_tablet=”0px” header_6_text_shadow_vertical_length_tablet=”0px” header_6_text_shadow_blur_strength_tablet=”1px” box_shadow_horizontal_tablet=”0px” box_shadow_vertical_tablet=”0px” box_shadow_blur_tablet=”40px” box_shadow_spread_tablet=”0px” global_colors_info=”{}” theme_builder_area=”post_content” sticky_enabled=”0″]<\/p>\n

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GCSF ADA (granulocyte colony stimulating factor) ELISA | EL-141-73196<\/strong><\/span><\/h1>\n<\/div>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.16″ vertical_offset_tablet=”0″ horizontal_offset_tablet=”0″ custom_margin=”1px|||||” z_index_tablet=”0″ border_width_bottom=”5px” box_shadow_horizontal_tablet=”0px” box_shadow_vertical_tablet=”0px” box_shadow_blur_tablet=”40px” box_shadow_spread_tablet=”0px” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_row column_structure=”1_2,1_2″ _builder_version=”4.16″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_column type=”1_2″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||” theme_builder_area=”post_content”][et_pb_image src=”http:\/\/itsbio.co.kr\/wp-content\/uploads\/2021\/01\/ELISAs.png” align=”center” _builder_version=”4.16″ vertical_offset_tablet=”0″ horizontal_offset_tablet=”0″ width=”100%” max_width=”100%” custom_padding=”0px||0px|||” z_index_tablet=”0″ box_shadow_horizontal_tablet=”0px” box_shadow_vertical_tablet=”0px” box_shadow_blur_tablet=”40px” box_shadow_spread_tablet=”0px” global_colors_info=”{}” theme_builder_area=”post_content”][\/et_pb_image][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||” theme_builder_area=”post_content”][et_pb_text _builder_version=”4.23″ vertical_offset_tablet=”0″ horizontal_offset_tablet=”0″ hover_enabled=”0″ z_index_tablet=”0″ text_text_shadow_horizontal_length_tablet=”0px” text_text_shadow_vertical_length_tablet=”0px” text_text_shadow_blur_strength_tablet=”1px” link_text_shadow_horizontal_length_tablet=”0px” link_text_shadow_vertical_length_tablet=”0px” link_text_shadow_blur_strength_tablet=”1px” ul_text_shadow_horizontal_length_tablet=”0px” ul_text_shadow_vertical_length_tablet=”0px” ul_text_shadow_blur_strength_tablet=”1px” ol_text_shadow_horizontal_length_tablet=”0px” ol_text_shadow_vertical_length_tablet=”0px” ol_text_shadow_blur_strength_tablet=”1px” quote_text_shadow_horizontal_length_tablet=”0px” quote_text_shadow_vertical_length_tablet=”0px” quote_text_shadow_blur_strength_tablet=”1px” header_text_shadow_horizontal_length_tablet=”0px” header_text_shadow_vertical_length_tablet=”0px” header_text_shadow_blur_strength_tablet=”1px” header_2_text_shadow_horizontal_length_tablet=”0px” header_2_text_shadow_vertical_length_tablet=”0px” header_2_text_shadow_blur_strength_tablet=”1px” header_3_text_shadow_horizontal_length_tablet=”0px” header_3_text_shadow_vertical_length_tablet=”0px” header_3_text_shadow_blur_strength_tablet=”1px” header_4_text_shadow_horizontal_length_tablet=”0px” header_4_text_shadow_vertical_length_tablet=”0px” header_4_text_shadow_blur_strength_tablet=”1px” header_5_text_shadow_horizontal_length_tablet=”0px” header_5_text_shadow_vertical_length_tablet=”0px” header_5_text_shadow_blur_strength_tablet=”1px” header_6_text_shadow_horizontal_length_tablet=”0px” header_6_text_shadow_vertical_length_tablet=”0px” header_6_text_shadow_blur_strength_tablet=”1px” box_shadow_horizontal_tablet=”0px” box_shadow_vertical_tablet=”0px” box_shadow_blur_tablet=”40px” box_shadow_spread_tablet=”0px” global_colors_info=”{}” theme_builder_area=”post_content” sticky_enabled=”0″]<\/p>\n

CATALOG NUMBER :\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 <\/span><\/span>EL-141-73196<\/span><\/strong><\/p>\n

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The AffinityImmuno GCSF ADA ELISA kit contains the components required for measuring antibodies to GCSF in biological matrices such as human serum, plasma or cell culture supernatant.<\/span><\/h3>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.16″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_column type=”4_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||” theme_builder_area=”post_content”][et_pb_text _builder_version=”4.23″ vertical_offset_tablet=”0″ horizontal_offset_tablet=”0″ custom_margin=”||71px|||” hover_enabled=”0″ z_index_tablet=”0″ text_text_shadow_horizontal_length_tablet=”0px” text_text_shadow_vertical_length_tablet=”0px” text_text_shadow_blur_strength_tablet=”1px” link_text_shadow_horizontal_length_tablet=”0px” link_text_shadow_vertical_length_tablet=”0px” link_text_shadow_blur_strength_tablet=”1px” ul_text_shadow_horizontal_length_tablet=”0px” ul_text_shadow_vertical_length_tablet=”0px” ul_text_shadow_blur_strength_tablet=”1px” ol_text_shadow_horizontal_length_tablet=”0px” ol_text_shadow_vertical_length_tablet=”0px” ol_text_shadow_blur_strength_tablet=”1px” quote_text_shadow_horizontal_length_tablet=”0px” quote_text_shadow_vertical_length_tablet=”0px” quote_text_shadow_blur_strength_tablet=”1px” header_text_shadow_horizontal_length_tablet=”0px” header_text_shadow_vertical_length_tablet=”0px” header_text_shadow_blur_strength_tablet=”1px” header_2_text_shadow_horizontal_length_tablet=”0px” header_2_text_shadow_vertical_length_tablet=”0px” header_2_text_shadow_blur_strength_tablet=”1px” header_3_text_shadow_horizontal_length_tablet=”0px” header_3_text_shadow_vertical_length_tablet=”0px” header_3_text_shadow_blur_strength_tablet=”1px” header_4_text_shadow_horizontal_length_tablet=”0px” header_4_text_shadow_vertical_length_tablet=”0px” header_4_text_shadow_blur_strength_tablet=”1px” header_5_text_shadow_horizontal_length_tablet=”0px” header_5_text_shadow_vertical_length_tablet=”0px” header_5_text_shadow_blur_strength_tablet=”1px” header_6_text_shadow_horizontal_length_tablet=”0px” header_6_text_shadow_vertical_length_tablet=”0px” header_6_text_shadow_blur_strength_tablet=”1px” box_shadow_horizontal_tablet=”0px” box_shadow_vertical_tablet=”0px” box_shadow_blur_tablet=”40px” box_shadow_spread_tablet=”0px” global_colors_info=”{}” theme_builder_area=”post_content” sticky_enabled=”0″]<\/p>\n

INTRODUCTION<\/strong><\/span><\/h5>\n

 <\/p>\n

The AffinityImmuno GCSF ADA ELISA kit contains the components required for measuring antibodies to GCSF in biological matrices such as human serum, plasma or cell culture supernatant.<\/span><\/p>\n

 <\/p>\n

PRINCIPLE OF THE ASSAY<\/strong><\/span><\/h5>\n

 <\/p>\n

This assay employs the indirect enzyme immunoassay technique. GCSF is coated onto a 96 well microplate. Calibrator and test samples are prepared by dilution into assay buffer and are pipetted into the appropriate wells. Antibodies to GCSF present in biological matrices are bound to the immobilized GCSF. After washing away any unbound substances,
antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color
development is proportional to the amount of antibodies to GCSF present in the calibrator and test samples. The color development is stopped and the intensity of the color is measured.<\/span><\/p>\n

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\u00a0<\/h3>\n

Product Specifications<\/span><\/strong><\/h3>\n

 <\/p>\n<\/div>\n<\/div>\n

\n
\n
\n
\u00a0<\/div>\n
    \n
  • \n
    \n
    \n
    Application(s)<\/span>
    Measure anti-GCSF antibodies<\/h6>\n

     <\/p>\n<\/div>\n<\/div>\n<\/li>\n

  • \n
    \n
    \n
    Target Protein<\/span>
    GCSF (granulocyte colony stimulating factor)<\/h6>\n

     <\/p>\n<\/div>\n<\/div>\n<\/li>\n

  • \n
    \n
    \n
    Alternative Name of Target<\/span>
    Filgrastim<\/h6>\n

     <\/p>\n<\/div>\n<\/div>\n<\/li>\n

  • \n
    \n
    \n
    Size<\/span>
    96 wells<\/h6>\n

     <\/p>\n<\/div>\n<\/div>\n<\/li>\n

  • \n
    \n
    \n
    Precision<\/span>
    Intra-assay coefficient variation (CV) <20%, inter-assay CV <20%<\/h6>\n

     <\/p>\n<\/div>\n<\/div>\n<\/li>\n

  • \n
    \n
    \n
    Detection Limit<\/span>
    45 ng\/mL<\/h6>\n

     <\/p>\n<\/div>\n<\/div>\n<\/li>\n

  • \n
    \n
    \n
    Storage Conditions<\/span>
    Stable at -20\u00b0C<\/h6>\n

     <\/p>\n<\/div>\n<\/div>\n<\/li>\n

  • \n
    \n
    \n
    Safety Warnings and Precautions<\/span>
    Research Use Only. Follow instructions.<\/h6>\n

     <\/p>\n<\/div>\n<\/div>\n<\/li>\n

  • \n
    \n
    \n
    Each Kit Includes<\/span><\/h6>\n
    – Coated microtiter plate-96 wells<\/h6>\n
    – Calibrator diluent 1.8 ml<\/h6>\n
    – Calibrator diluent (1.15mg\/mL -12 \u03bcl)<\/h6>\n
    – 10X wash buffer-50 ml<\/h6>\n
    – Assay buffer-50 ml<\/h6>\n
    – 1000X detection reagent-17 \u03bcl<\/h6>\n
    – TMB -12 ml<\/h6>\n
    – TMB stop solution-12 ml<\/h6>\n<\/div>\n<\/div>\n<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n

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