{"id":16357,"date":"2023-10-13T13:46:14","date_gmt":"2023-10-13T04:46:14","guid":{"rendered":"http:\/\/itsbio.co.kr\/?page_id=16357"},"modified":"2023-12-28T11:57:04","modified_gmt":"2023-12-28T02:57:04","slug":"speedy-lentivirus-purification-2","status":"publish","type":"page","link":"http:\/\/itsbio.co.kr\/?page_id=16357","title":{"rendered":"Speedy Lentivirus Purification"},"content":{"rendered":"<p>[et_pb_section fb_built=&#8221;1&#8243; _builder_version=&#8221;4.16&#8243; vertical_offset_tablet=&#8221;0&#8243; horizontal_offset_tablet=&#8221;0&#8243; z_index_tablet=&#8221;0&#8243; custom_css_main_element=&#8221;padding:0;||margin:0;&#8221; box_shadow_horizontal_tablet=&#8221;0px&#8221; box_shadow_vertical_tablet=&#8221;0px&#8221; box_shadow_blur_tablet=&#8221;40px&#8221; box_shadow_spread_tablet=&#8221;0px&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_row _builder_version=&#8221;4.16&#8243; vertical_offset_tablet=&#8221;0&#8243; horizontal_offset_tablet=&#8221;0&#8243; z_index_tablet=&#8221;0&#8243; 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header_3_text_shadow_blur_strength_tablet=&#8221;1px&#8221; header_4_text_shadow_horizontal_length_tablet=&#8221;0px&#8221; header_4_text_shadow_vertical_length_tablet=&#8221;0px&#8221; header_4_text_shadow_blur_strength_tablet=&#8221;1px&#8221; header_5_text_shadow_horizontal_length_tablet=&#8221;0px&#8221; header_5_text_shadow_vertical_length_tablet=&#8221;0px&#8221; header_5_text_shadow_blur_strength_tablet=&#8221;1px&#8221; header_6_text_shadow_horizontal_length_tablet=&#8221;0px&#8221; header_6_text_shadow_vertical_length_tablet=&#8221;0px&#8221; header_6_text_shadow_blur_strength_tablet=&#8221;1px&#8221; box_shadow_horizontal_tablet=&#8221;0px&#8221; box_shadow_vertical_tablet=&#8221;0px&#8221; box_shadow_blur_tablet=&#8221;40px&#8221; box_shadow_spread_tablet=&#8221;0px&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p style=\"font-size: 1.8em; line-height: 200px;\"><span style=\"color: #000000;\"><strong>Speedy Lentivirus Purification<br \/>\n<\/strong><\/span><\/p>\n<p>[\/et_pb_text][et_pb_text admin_label=&#8221;\ud14d\uc2a4\ud2b8&#8221; 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No.<\/span><\/strong><\/div>\n<\/th>\n<th>LV999<\/th>\n<\/tr>\n<\/thead>\n<tfoot>\n<tr style=\"border-color: #ef6331;\">\n<td style=\"text-align: center;\"><strong><span style=\"color: #000000;\">Unit<\/span><\/strong><\/td>\n<td>\n<div class=\"row row-no-gutters abm-mb-1 show-info\">\n<div class=\"col-xs-7 col-md-9\">100 ml<\/div>\n<\/div>\n<\/td>\n<\/tr>\n<\/tfoot>\n<\/table>\n<\/div>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;4_4&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.22.2&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;]<style id=\"sp_tab_dynamic_style16361\">#sp-tabpro-wrapper_16361.sp-tab__lay-default .sp-tab__tab-content {width: 100%;}#sp-tabpro-wrapper_16361 .animated 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#444;}<\/style>\t\t<div id=\"sp-wp-tabs-wrapper_16361\" class=\"sp-tab__lay-default\" data-preloader=\"1\" data-activemode=\"tabs-activator-event-click\">\n\t\t\t<div class=\"sp-tab__preloader\">\n\t\t<div class=\"sp-tab__spinner\">\n\t\t\t<div class=\"rect1\"><\/div>\n\t\t\t<div class=\"rect2\"><\/div>\n\t\t\t<div class=\"rect3\"><\/div>\n\t\t\t<div class=\"rect4\"><\/div>\n\t\t\t<div class=\"rect5\"><\/div>\n\t\t<\/div>\n\t<\/div>\n\t<ul class=\"sp-tab__nav sp-tab__nav-tabs\" id=\"sp-tab__ul\" role=\"tablist\">\n\t\t\t\t<li class=\"sp-tab__nav-item\" role=\"tab\">\n\t\t\t\t<label class=\"sp-tab__nav-link sp-tab__active\" data-sptoggle=\"tab\" for=\"#tab-163611\" role=\"tab\" aria-controls=tab-163611 aria-selected=true tabindex=0>\n\t\t\t\t\t<span class=\"tab_title_area\"><H3 class=\"sp-tab__tab_title\">Specifications<\/H3><\/span>\n\t\t\t\t<\/label>\n\t\t\t<\/li>\n\t\t\t\t\t\t<li class=\"sp-tab__nav-item\" role=\"tab\">\n\t\t\t\t<label class=\"sp-tab__nav-link\" data-sptoggle=\"tab\" for=\"#tab-163612\" role=\"tab\" aria-controls=tab-163612 aria-selected=true tabindex=0>\n\t\t\t\t\t<span class=\"tab_title_area\"><H3 class=\"sp-tab__tab_title\">Datasheet<\/H3><\/span>\n\t\t\t\t<\/label>\n\t\t\t<\/li>\n\t\t\t\t\t\t<li class=\"sp-tab__nav-item\" role=\"tab\">\n\t\t\t\t<label class=\"sp-tab__nav-link\" data-sptoggle=\"tab\" for=\"#tab-163613\" role=\"tab\" aria-controls=tab-163613 aria-selected=true tabindex=0>\n\t\t\t\t\t<span class=\"tab_title_area\"><H3 class=\"sp-tab__tab_title\">Documents<\/H3><\/span>\n\t\t\t\t<\/label>\n\t\t\t<\/li>\n\t\t\t\t\t\t<li class=\"sp-tab__nav-item\" role=\"tab\">\n\t\t\t\t<label class=\"sp-tab__nav-link\" data-sptoggle=\"tab\" for=\"#tab-163614\" role=\"tab\" aria-controls=tab-163614 aria-selected=true tabindex=0>\n\t\t\t\t\t<span class=\"tab_title_area\"><H3 class=\"sp-tab__tab_title\">FAQs<\/H3><\/span>\n\t\t\t\t<\/label>\n\t\t\t<\/li>\n\t\t\t\t\t\t<li class=\"sp-tab__nav-item\" role=\"tab\">\n\t\t\t\t<label class=\"sp-tab__nav-link\" data-sptoggle=\"tab\" for=\"#tab-163615\" role=\"tab\" aria-controls=tab-163615 aria-selected=true tabindex=0>\n\t\t\t\t\t<span class=\"tab_title_area\"><H3 class=\"sp-tab__tab_title\">References<\/H3><\/span>\n\t\t\t\t<\/label>\n\t\t\t<\/li>\n\t\t\t\t\t\t<li class=\"sp-tab__nav-item\" role=\"tab\">\n\t\t\t\t<label class=\"sp-tab__nav-link\" data-sptoggle=\"tab\" for=\"#tab-163616\" role=\"tab\" aria-controls=tab-163616 aria-selected=true tabindex=0>\n\t\t\t\t\t<span class=\"tab_title_area\"><H3 class=\"sp-tab__tab_title\">Reviews<\/H3><\/span>\n\t\t\t\t<\/label>\n\t\t\t<\/li>\n\t\t\t<\/ul>\n<div class=\"sp-tab__tab-content\">\n\t\t\t\t<div id=\"tab-163611\" class=\"sp-tab__tab-pane sp-tab__show sp-tab__active\" role=\"tabpanel\">\n\t\t\t\t\t<div class=\"sp-tab-content animated fadeIn\"><table>\n<tbody>\n<tr class=\"abm-specifications-cat_no\">\n<td class=\"abm-specifications-label\" valign=\"top\">Cat. No.<\/td>\n<td class=\"abm-specifications-value\" valign=\"top\"><span id=\"abm-specifications-cat_no\">LV999<\/span><\/td>\n<\/tr>\n<tr class=\"abm-specifications-name\">\n<td class=\"abm-specifications-label\" valign=\"top\">Name<\/td>\n<td class=\"abm-specifications-value\" valign=\"top\">Speedy Lentivirus Purification<\/td>\n<\/tr>\n<tr class=\"abm-specifications-unit_quantity\">\n<td class=\"abm-specifications-label\" valign=\"top\">Unit<\/td>\n<td class=\"abm-specifications-value\" valign=\"top\">100 ml<\/td>\n<\/tr>\n<tr class=\"abm-specifications-description\">\n<td class=\"abm-specifications-label\" valign=\"top\">Description<\/td>\n<td class=\"abm-specifications-value\" valign=\"top\">Recombinant lentiviral vectors have been shown to be a powerful tool for stable gene transfer to both dividing and non-dividing cells in vitro and in vivo.Through years of experience with lentiviral vectors, ABM has developed its own proprietary pLenti-combo packaging mix and an efficient protocol for rapid production of recombinant lentiviral vectors with titers up to 10<sup>7<\/sup>IU\/ml. The quickest and easiest way to purify and concentrate lentiviral particles up to 100x.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<div id=\"tab-163612\" class=\"sp-tab__tab-pane \" role=\"tabpanel\">\n\t\t\t\t\t<div class=\"sp-tab-content animated fadeIn\"><div class=\"abm-doc-title\">Datasheet<\/div>\n<ul class=\"abm-document-list\">\n<li><span style=\"color: #ff6600\"><a style=\"color: #ff6600\" href=\"https:\/\/www.abmgood.com\/uploads\/document\/lv999-datasheet%202023.pdf\" target=\"_blank\" rel=\"noopener\">Speedy Lentivirus Purification LV999 Datasheet<\/a><\/span><\/li>\n<\/ul>\n<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<div id=\"tab-163613\" class=\"sp-tab__tab-pane \" role=\"tabpanel\">\n\t\t\t\t\t<div class=\"sp-tab-content animated fadeIn\"><div class=\"abm-doc-div\">\n<div class=\"abm-doc-title\">Search CoA\u00a0<a class=\"abm-link\" href=\"https:\/\/www.abmgood.com\/CoA-Library.html\" target=\"_blank\" rel=\"noopener\">here<\/a><\/div>\n<\/div>\n<div class=\"abm-doc-div\">\n<div class=\"abm-doc-title\">Supporting Protocol<\/div>\n<ul class=\"abm-document-list\" data-count=\"1\">\n<li><a href=\"https:\/\/www.abmgood.com\/document\/Drug%20selection%20killing%20curve%20Final_111315.pdf\" target=\"_blank\" rel=\"noopener\"><span style=\"color: #ff6600\">Selection-Drug Killing Curve<\/span><\/a><\/li>\n<li><span style=\"color: #ff6600\"><a style=\"color: #ff6600\" href=\"https:\/\/www.abmgood.com\/uploads\/document\/A4_lenti_packaging_2023_V2.pdf\" target=\"_blank\" rel=\"noopener\">Lentivector Packaging Protocol<\/a><\/span><\/li>\n<li><span style=\"color: #ff6600\"><a style=\"color: #ff6600\" href=\"https:\/\/www.abmgood.com\/uploads\/document\/a4_lenti_infection-with_spinoculation_.pdf\" target=\"_blank\" rel=\"noopener\">Lentivirus Infection Protocol<\/a><\/span><\/li>\n<li><span style=\"color: #ff6600\"><a style=\"color: #ff6600\" href=\"https:\/\/www.abmgood.com\/document\/LV999-Protocol_V1.pdf\" target=\"_blank\" rel=\"noopener\">Speedy Lentivirus Purification Protocol<\/a><\/span><\/li>\n<\/ul>\n<\/div>\n<div class=\"abm-doc-div\">\n<div class=\"abm-doc-title\">Other<\/div>\n<ul class=\"abm-document-list\" data-count=\"1\">\n<li><span style=\"color: #ff6600\"><a style=\"color: #ff6600\" href=\"https:\/\/www.abmgood.com\/uploads\/document\/Lentivirus%20incompetency-abm.pdf\" target=\"_blank\" rel=\"noopener\">Enhanced Lentivirus Safety Features: Replication Incompetency<\/a><\/span><\/li>\n<li><span style=\"color: #ff6600\"><a style=\"color: #ff6600\" href=\"https:\/\/www.abmgood.com\/uploads\/document\/A4%20%20%20lenti%20infection%20%20Cancer%20Cell%20Line%20Guide.pdf\" target=\"_blank\" rel=\"noopener\">Suggested MOI for Common Cancer Cell Lines<\/a><\/span><\/li>\n<li><span style=\"color: #ff6600\"><a style=\"color: #ff6600\" href=\"https:\/\/www.abmgood.com\/document\/\/l\/e\/lentiviral-system-faq.pdf\" target=\"_blank\" rel=\"noopener\">Lentiviral System FAQ<\/a><\/span><\/li>\n<\/ul>\n<\/div>\n<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<div id=\"tab-163614\" class=\"sp-tab__tab-pane \" role=\"tabpanel\">\n\t\t\t\t\t<div class=\"sp-tab-content animated fadeIn\"><div class=\"product-info-box abm-mt-5 abm-pt-5\">\n<div class=\"tab-content\">\n<div id=\"faqs\" class=\"tab-pane active\" role=\"tabpanel\">\n<div class=\"abm-doc-div\"><style>#sp-ea-16362 .spcollapsing { height: 0; overflow: hidden; transition-property: height;transition-duration: 500ms;}#sp-ea-16362.sp-easy-accordion>.sp-ea-single {border: 1px solid #e2e2e2; }#sp-ea-16362.sp-easy-accordion>.sp-ea-single>.ea-header a {color: #444;}#sp-ea-16362.sp-easy-accordion>.sp-ea-single>.sp-collapse>.ea-body {background: #fff; color: #444;}#sp-ea-16362.sp-easy-accordion>.sp-ea-single {background: #eee;}#sp-ea-16362.sp-easy-accordion>.sp-ea-single>.ea-header a .ea-expand-icon.fa { float: left; color: #444;font-size: 16px;}<\/style><div id=\"sp_easy_accordion-1782566630\"><div id=\"sp-ea-16362\" class=\"sp-ea-one sp-easy-accordion\" data-ex-icon=\"fa-minus\" data-col-icon=\"fa-plus\"  data-ea-active=\"ea-click\"  data-ea-mode=\"vertical\" data-preloader=\"\" data-scroll-active-item=\"\" data-offset-to-scroll=\"0\"><div class=\"ea-card ea-expand sp-ea-single\"><h3 class=\"ea-header\"><a class=\"collapsed\" data-sptoggle=\"spcollapse\" data-sptarget=#collapse163620 href=\"javascript:void(0)\"  aria-expanded=\"true\"><i class=\"ea-expand-icon fa fa-minus\"><\/i> What is the mechanism of purification for the Speedy Lentivirus Purification?<\/a><\/h3><div class=\"sp-collapse spcollapse collapsed show\" id=\"collapse163620\" data-parent=#sp-ea-16362><div class=\"ea-body\"><p>The Speedy Lentivirus Purification is a positively charged matrix solution that binds to the negatively charged lentivirus, forming a precipitation that can be pelleted through centrifugation.<\/p>\n<\/div><\/div><\/div><div class=\"ea-card  sp-ea-single\"><h3 class=\"ea-header\"><a class=\"collapsed\" data-sptoggle=\"spcollapse\" data-sptarget=#collapse163621 href=\"javascript:void(0)\"  aria-expanded=\"false\"><i class=\"ea-expand-icon fa fa-plus\"><\/i> Can it also be used for Retrovirus purification?<\/a><\/h3><div class=\"sp-collapse spcollapse \" id=\"collapse163621\" data-parent=#sp-ea-16362><div class=\"ea-body\"><p>No, because retroviruses are too sensitive to survive the required centrifugation step.<\/p>\n<\/div><\/div><\/div><div class=\"ea-card  sp-ea-single\"><h3 class=\"ea-header\"><a class=\"collapsed\" data-sptoggle=\"spcollapse\" data-sptarget=#collapse163622 href=\"javascript:void(0)\"  aria-expanded=\"false\"><i class=\"ea-expand-icon fa fa-plus\"><\/i> Will the lack of FBS and calcium affect the functionality of the speedy lentivirus purification?<\/a><\/h3><div class=\"sp-collapse spcollapse \" id=\"collapse163622\" data-parent=#sp-ea-16362><div class=\"ea-body\"><p>No, the function will not be affected.<\/p>\n<\/div><\/div><\/div><div class=\"ea-card  sp-ea-single\"><h3 class=\"ea-header\"><a class=\"collapsed\" data-sptoggle=\"spcollapse\" data-sptarget=#collapse163623 href=\"javascript:void(0)\"  aria-expanded=\"false\"><i class=\"ea-expand-icon fa fa-plus\"><\/i> What buffer should I use for resuspension?<\/a><\/h3><div class=\"sp-collapse spcollapse \" id=\"collapse163623\" data-parent=#sp-ea-16362><div class=\"ea-body\"><p>The pelleted lentivirus can be resuspended in any appropriate buffer.<\/p>\n<\/div><\/div><\/div><div class=\"ea-card  sp-ea-single\"><h3 class=\"ea-header\"><a class=\"collapsed\" data-sptoggle=\"spcollapse\" data-sptarget=#collapse163624 href=\"javascript:void(0)\"  aria-expanded=\"false\"><i class=\"ea-expand-icon fa fa-plus\"><\/i> Can I remove the matrix as much as possible from the resuspended lentivirus?<\/a><\/h3><div class=\"sp-collapse spcollapse \" id=\"collapse163624\" data-parent=#sp-ea-16362><div class=\"ea-body\"><p>Yes it is possible but the lentivirus should go through a buffer exchange procedure. Our recommended method is loading the resuspended lentivirus onto a 20mL-50uL ultrafiltration unit (30,000 MWCO), add PBS, then wash out the matrix by multiple centrifuge spins and discard the flowthrough. Once the exchange has occurred, use the desired volume of solvent to resuspend the lentivirus from the top filter and aliquot into storage containers.<\/p>\n<\/div><\/div><\/div><div class=\"ea-card  sp-ea-single\"><h3 class=\"ea-header\"><a class=\"collapsed\" data-sptoggle=\"spcollapse\" data-sptarget=#collapse163625 href=\"javascript:void(0)\"  aria-expanded=\"false\"><i class=\"ea-expand-icon fa fa-plus\"><\/i> What is the difference between Retro-, Lenti-, and Adeno- viruses?<\/a><\/h3><div class=\"sp-collapse spcollapse \" id=\"collapse163625\" data-parent=#sp-ea-16362><div class=\"ea-body\"><p>Retrovirus: Classic, can integrate into the genome but with low transduction efficiency. They are useful for gene transfer and protein expression in cells that have low transfection efficiency with other transfection reagents. Lentivirus: Can integrate into the genome with relatively high transduction efficiency and they are very useful for cells that have low transfection efficiency with other transfection reagents. No special competent cells required, as they are stable plasmids. Lentiviruses are a powerful tool for stable gene transfer to both dividing and non-dividing cells in vitro and in vivo. Adenovirus: Only work transiently (about 7 days) but have almost 100% transduction efficiency. Adenoviruses can infect a broad range of cell types with the highest efficiency and infection is not dependent on active host cell division. A second key feature is that high virus titers and high-level gene expression can be obtained in most mammalian cells.<\/p>\n<\/div><\/div><\/div><div class=\"ea-card  sp-ea-single\"><h3 class=\"ea-header\"><a class=\"collapsed\" data-sptoggle=\"spcollapse\" data-sptarget=#collapse163626 href=\"javascript:void(0)\"  aria-expanded=\"false\"><i class=\"ea-expand-icon fa fa-plus\"><\/i> What are the correct concentration units for each recombinant viral particle?<\/a><\/h3><div class=\"sp-collapse spcollapse \" id=\"collapse163626\" data-parent=#sp-ea-16362><div class=\"ea-body\"><p>For lentiviruses and retroviruses, they are measured in CFU\/ml (colony-forming units per millilitre). Transduction with lentiviruses and retroviruses can cause the formation of colonies, which can be quantified for concentration. For AAV the titer is measured as genome copies per mL (GC\/mL). Adenoviruses are measured as PFU\/ml (plaque-forming units per millilitre). Transduction with adenoviruses will kill packaging cells, forming plaques in the process for quantification. The concentration for all three types of viruses can also be classified as IU\/ml (Infectious Units\/ml). Ultimately, the units refers to the viral particles and different units reflect the different assays involved.<\/p>\n<\/div><\/div><\/div><div class=\"ea-card  sp-ea-single\"><h3 class=\"ea-header\"><a class=\"collapsed\" data-sptoggle=\"spcollapse\" data-sptarget=#collapse163627 href=\"javascript:void(0)\"  aria-expanded=\"false\"><i class=\"ea-expand-icon fa fa-plus\"><\/i> What do I use to check if my cells were successfully immortalized by the SV40 agent?<\/a><\/h3><div class=\"sp-collapse spcollapse \" id=\"collapse163627\" data-parent=#sp-ea-16362><div class=\"ea-body\"><p>We have an SV40 T antibody that can be used for the western blot analysis. The catalog number is G202. Otherwise, a qPCR primer can be designed on the SV40 gene for qPCR analysis. The sequence can be found in the link below: http:\/\/www.abmgood.com\/pLenti%20SV40-Vector-Location-Map.html<\/p>\n<\/div><\/div><\/div><div class=\"ea-card  sp-ea-single\"><h3 class=\"ea-header\"><a class=\"collapsed\" data-sptoggle=\"spcollapse\" data-sptarget=#collapse163628 href=\"javascript:void(0)\"  aria-expanded=\"false\"><i class=\"ea-expand-icon fa fa-plus\"><\/i> What filter can I used for filter sterilization after the purification step?<\/a><\/h3><div class=\"sp-collapse spcollapse \" id=\"collapse163628\" data-parent=#sp-ea-16362><div class=\"ea-body\"><p>We recommend a low protein binding filter with membrane pores of 0.2-0.45um. A few suggested membrane materials are PVDF, PES, and Supor (from Pall).<\/p>\n<\/div><\/div><\/div><div class=\"ea-card  sp-ea-single\"><h3 class=\"ea-header\"><a class=\"collapsed\" data-sptoggle=\"spcollapse\" data-sptarget=#collapse163629 href=\"javascript:void(0)\"  aria-expanded=\"false\"><i class=\"ea-expand-icon fa fa-plus\"><\/i> What are the primers to use for SV40 identification?<\/a><\/h3><div class=\"sp-collapse spcollapse \" id=\"collapse163629\" data-parent=#sp-ea-16362><div class=\"ea-body\"><p>SV40 Forward Primer Sequence 5\u2019 ACTGAGGGGCCTGAAATGA SV40 Reverse Primer Sequence 5\u2019 GACTCAGGGCATGAAACAGG These are qPCR primers and the band size is 61 bp.<\/p>\n<\/div><\/div><\/div><div class=\"ea-card  sp-ea-single\"><h3 class=\"ea-header\"><a class=\"collapsed\" data-sptoggle=\"spcollapse\" data-sptarget=#collapse1636210 href=\"javascript:void(0)\"  aria-expanded=\"false\"><i class=\"ea-expand-icon fa fa-plus\"><\/i> What advantages \/ disadvantages exist between the Lenti-SV40, -SV40T, and SV40T+t vectors?<\/a><\/h3><div class=\"sp-collapse spcollapse \" id=\"collapse1636210\" data-parent=#sp-ea-16362><div class=\"ea-body\"><p>There are simply differences in the content of all vectors due to customer demand for variety. Lenti-SV40 will contain the whole SV40 gene, -SV40T, the large T Antigen only, and -SV40T&t the large and small T antigens only. It is up to the end user to decide which vectors will best suit their project, however we have successfully used Lenti-SV40 (whole gene) in a wide range of immortalization projects.<\/p>\n<\/div><\/div><\/div><div class=\"ea-card  sp-ea-single\"><h3 class=\"ea-header\"><a class=\"collapsed\" data-sptoggle=\"spcollapse\" data-sptarget=#collapse1636211 href=\"javascript:void(0)\"  aria-expanded=\"false\"><i class=\"ea-expand-icon fa fa-plus\"><\/i> What is the accession number for the SV40?<\/a><\/h3><div class=\"sp-collapse spcollapse \" id=\"collapse1636211\" data-parent=#sp-ea-16362><div class=\"ea-body\"><p>The SV40 covers the entire genome and the accession number is J02400.1. You can use this information to design primers for conventional PCR as well.<\/p>\n<\/div><\/div><\/div><div class=\"ea-card  sp-ea-single\"><h3 class=\"ea-header\"><a class=\"collapsed\" data-sptoggle=\"spcollapse\" data-sptarget=#collapse1636212 href=\"javascript:void(0)\"  aria-expanded=\"false\"><i class=\"ea-expand-icon fa fa-plus\"><\/i> How long after transduction can the infection efficiency be observed?<\/a><\/h3><div class=\"sp-collapse spcollapse \" id=\"collapse1636212\" data-parent=#sp-ea-16362><div class=\"ea-body\"><p>You can observe transduction efficiency from 48 hours up to 5 days after infection.<\/p>\n<\/div><\/div><\/div><div class=\"ea-card  sp-ea-single\"><h3 class=\"ea-header\"><a class=\"collapsed\" data-sptoggle=\"spcollapse\" data-sptarget=#collapse1636213 href=\"javascript:void(0)\"  aria-expanded=\"false\"><i class=\"ea-expand-icon fa fa-plus\"><\/i> What are the primers to use for SV40T and SV40T tsA58 detection?<\/a><\/h3><div class=\"sp-collapse spcollapse \" id=\"collapse1636213\" data-parent=#sp-ea-16362><div class=\"ea-body\"><p>PCR primers: SV40T Forward Primer Sequence 5\u2019 AGCCTGTAGAACCAAACATT 3' SV40T Reverse Primer Sequence 5\u2019 CTGCTGACTCTCAACATTCT 3' The two primers should amplify the region between 3677-4468bp, giving a 792bp fragment.<\/p>\n<\/div><\/div><\/div><div class=\"ea-card  sp-ea-single\"><h3 class=\"ea-header\"><a class=\"collapsed\" data-sptoggle=\"spcollapse\" data-sptarget=#collapse1636214 href=\"javascript:void(0)\"  aria-expanded=\"false\"><i class=\"ea-expand-icon fa fa-plus\"><\/i>  What is the sequence of the SV40 large T antigen?<\/a><\/h3><div class=\"sp-collapse spcollapse \" id=\"collapse1636214\" data-parent=#sp-ea-16362><div class=\"ea-body\"><p>This information can be accessed on this page by clicking on \"pLenti-SV40-T\" under vector map. The Large T antigen is at position 5079-5927.<\/p>\n<\/div><\/div><\/div><div class=\"ea-card  sp-ea-single\"><h3 class=\"ea-header\"><a class=\"collapsed\" data-sptoggle=\"spcollapse\" data-sptarget=#collapse1636215 href=\"javascript:void(0)\"  aria-expanded=\"false\"><i class=\"ea-expand-icon fa fa-plus\"><\/i> In the final solution what medium and FBS should I use to keep the concentrated viruses?<\/a><\/h3><div class=\"sp-collapse spcollapse \" id=\"collapse1636215\" data-parent=#sp-ea-16362><div class=\"ea-body\"><p>When using our Speedy Lentivirus Purification (Cat#LV999), the choice of which medium and FBS to use to keep the concentrated viruses will vary based upon the intended downstream application of the lentivirus; i.e. depending on the complete media required for your cell line of interest. You can re-suspend the pellet in the complete media of the cell line which will be infected with this virus prep, to avoid dilution. For example, if 293T cells were the intended cell line, we would recommend using DMEM + 10% FBS. If in doubt, serum-free DMEM is typically a safe choice, however the required dilution will be a factor to consider.<\/p>\n<\/div><\/div><\/div><div class=\"ea-card  sp-ea-single\"><h3 class=\"ea-header\"><a class=\"collapsed\" data-sptoggle=\"spcollapse\" data-sptarget=#collapse1636216 href=\"javascript:void(0)\"  aria-expanded=\"false\"><i class=\"ea-expand-icon fa fa-plus\"><\/i> For G221 and LV620, what does the 'V12' in RasV12 mean?<\/a><\/h3><div class=\"sp-collapse spcollapse \" id=\"collapse1636216\" data-parent=#sp-ea-16362><div class=\"ea-body\"><p>The V12 means that amino acid # 12 is mutated from a Valine to a Glycine. Other than that, the sequence matches the coding region of HRAS perfectly (NM_005343).<\/p>\n<\/div><\/div><\/div><div class=\"ea-card  sp-ea-single\"><h3 class=\"ea-header\"><a class=\"collapsed\" data-sptoggle=\"spcollapse\" data-sptarget=#collapse1636217 href=\"javascript:void(0)\"  aria-expanded=\"false\"><i class=\"ea-expand-icon fa fa-plus\"><\/i> Where is the SV40T tsA58 gene sequence?<\/a><\/h3><div class=\"sp-collapse spcollapse \" id=\"collapse1636217\" data-parent=#sp-ea-16362><div class=\"ea-body\"><p>The SV40T tsA58 gene is located between 3138-5264bp, with the Alanine-to-Valine mutation at amino acid 438.<\/p>\n<\/div><\/div><\/div><\/div><\/div><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<div id=\"tab-163615\" class=\"sp-tab__tab-pane \" role=\"tabpanel\">\n\t\t\t\t\t<div class=\"sp-tab-content animated fadeIn\"><div class=\"product-info-box abm-mt-5 abm-pt-5\">\n<div class=\"tab-content\">\n<div id=\"references\" class=\"tab-pane active\" role=\"tabpanel\">\n<div>\n<div class=\"abm-doc-div\">There are no references for this product yet!<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"abm-options-product\">\n<div class=\"abm-clear-div\"><\/div>\n<div class=\"col-sm-12 col-md-12 col-lg-12\"><\/div>\n<\/div>\n<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<div id=\"tab-163616\" class=\"sp-tab__tab-pane \" role=\"tabpanel\">\n\t\t\t\t\t<div class=\"sp-tab-content animated fadeIn\"><p>This product has no review yet.<\/p>\n<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=&#8221;1&#8243; admin_label=&#8221;\uc808&#8221; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; locked=&#8221;off&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_row _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; locked=&#8221;off&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;4_4&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text admin_label=&#8221;\ud14d\uc2a4\ud2b8&#8221; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p style=\"font-size: 25px;\"><span style=\"color: #000000;\"><strong>Controls and Related Product:\u00a0<\/strong><\/span><\/p>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=&#8221;1_4,1_4,1_4,1_4&#8243; admin_label=&#8221;\ud589&#8221; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; locked=&#8221;off&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;1_4&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.22.2&#8243; _module_preset=&#8221;default&#8221; locked=&#8221;off&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<style>\n.o{\nborder: 3px solid #ef6331;\nborder-radius: 12px;\ndisplay: inline-block;\npadding: 0.8em;\nwidth: 100%;\nheight: 280px;\n}\n<\/style>\n<div class=\"o\">\n<p><span style=\"color: #ff6600; font-size: 15px;\"><strong><a style=\"color: #ff6600;\" href=\"\/?page_id=10618\">DNAfectin\u2122 Plus Transfection Reagent<\/a><\/strong><\/span><\/p>\n<hr \/>\n<table style=\"border: none; border-color: #ef6331;\">\n<thead>\n<tr style=\"border-color: #ef6331;\">\n<th style=\"text-align: center; border-color: #ef6331;\">\n<div class=\"col-xs-5 col-md-3 abm-text-bold\"><strong><span style=\"color: #000000;\">Cat. No.<\/span><\/strong><\/div>\n<\/th>\n<th>\n<div class=\"abm-product-row col-sm-7\">\n<div class=\"abm-product-row col-sm-7\">G2500<\/div>\n<\/div>\n<\/th>\n<\/tr>\n<\/thead>\n<tfoot>\n<tr style=\"border-color: #ef6331;\">\n<td style=\"text-align: center;\"><strong><span style=\"color: #000000;\">Unit<\/span><\/strong><\/td>\n<td>\n<div class=\"abm-product-more-line\">\n<div class=\"abm-product-row col-sm-7\">1.0 ml<\/div>\n<\/div>\n<\/td>\n<\/tr>\n<\/tfoot>\n<\/table>\n<\/div>\n<p>[\/et_pb_text][et_pb_text _builder_version=&#8221;4.22.2&#8243; _module_preset=&#8221;default&#8221; locked=&#8221;off&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<div class=\"o\">\n<p><span style=\"color: #ff6600; font-size: 14px;\"><strong><a style=\"color: #ff6600;\" href=\"\/?page_id=6353\" data-wplink-edit=\"true\">2nd Generation Packaging System Mix<\/a><\/strong><\/span><\/p>\n<hr \/>\n<table style=\"border: none; border-color: #ef6331;\">\n<thead>\n<tr style=\"border-color: #ef6331;\">\n<th style=\"text-align: center; border-color: #ef6331;\">\n<div class=\"col-xs-5 col-md-3 abm-text-bold\"><strong><span style=\"color: #000000;\">Cat. No.<\/span><\/strong><\/div>\n<\/th>\n<th>\n<div class=\"abm-product-row col-sm-7\">\n<div class=\"abm-product-row col-sm-7\">\n<div class=\"abm-product-row col-sm-7\">LV003<\/div>\n<\/div>\n<\/div>\n<\/th>\n<\/tr>\n<\/thead>\n<tfoot>\n<tr style=\"border-color: #ef6331;\">\n<td style=\"text-align: center;\"><strong><span style=\"color: #000000;\">Unit<\/span><\/strong><\/td>\n<td>\n<div class=\"abm-product-more-line\">\n<div class=\"abm-product-row col-sm-7\">200\u00b5l<\/div>\n<\/div>\n<\/td>\n<\/tr>\n<\/tfoot>\n<\/table>\n<\/div>\n<p>[\/et_pb_text][\/et_pb_column][et_pb_column type=&#8221;1_4&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.22.2&#8243; _module_preset=&#8221;default&#8221; locked=&#8221;off&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<div class=\"o\">\n<p><span style=\"color: #ff6600; font-size: 14px;\"><strong><a style=\"color: #ff6600;\" href=\"\/?page_id=6525\" data-wplink-edit=\"true\">qPCR Lentivirus Titer Kit<\/a><\/strong><\/span><\/p>\n<hr \/>\n<table style=\"border: none; border-color: #ef6331;\">\n<thead>\n<tr style=\"border-color: #ef6331;\">\n<th style=\"text-align: center; border-color: #ef6331;\">\n<div class=\"col-xs-5 col-md-3 abm-text-bold\"><strong><span style=\"color: #000000;\">Cat. No.<\/span><\/strong><\/div>\n<\/th>\n<th>\n<div class=\"abm-product-row col-sm-7\">\n<div class=\"abm-product-row col-sm-7\">LV900<\/div>\n<\/div>\n<\/th>\n<\/tr>\n<\/thead>\n<tfoot>\n<tr style=\"border-color: #ef6331;\">\n<td style=\"text-align: center;\"><strong><span style=\"color: #000000;\">Unit<\/span><\/strong><\/td>\n<td>\n<div class=\"abm-product-more-line\">\n<div class=\"abm-product-row col-sm-7\">100 rxn<\/div>\n<\/div>\n<\/td>\n<\/tr>\n<\/tfoot>\n<\/table>\n<\/div>\n<p>[\/et_pb_text][et_pb_text _builder_version=&#8221;4.22.2&#8243; _module_preset=&#8221;default&#8221; locked=&#8221;off&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<div class=\"o\">\n<p><span style=\"color: #ff6600; font-size: 15px;\"><strong><a style=\"color: #ff6600;\" href=\"\/?page_id=6366\" data-wplink-edit=\"true\">3rd Generation Packaging System Mix<\/a><\/strong><\/span><\/p>\n<hr \/>\n<table style=\"border: none; border-color: #ef6331;\">\n<thead>\n<tr style=\"border-color: #ef6331;\">\n<th style=\"text-align: center; border-color: #ef6331;\">\n<div class=\"col-xs-5 col-md-3 abm-text-bold\"><strong><span style=\"color: #000000;\">Cat. No.<\/span><\/strong><\/div>\n<\/th>\n<th>\n<div class=\"abm-product-row col-sm-7\">\n<div class=\"abm-product-row col-sm-7\">\n<div class=\"abm-product-row col-sm-7\">LV053<\/div>\n<\/div>\n<\/div>\n<\/th>\n<\/tr>\n<\/thead>\n<tfoot>\n<tr style=\"border-color: #ef6331;\">\n<td style=\"text-align: center;\"><strong><span style=\"color: #000000;\">Unit<\/span><\/strong><\/td>\n<td>\n<div class=\"abm-product-more-line\">\n<div class=\"abm-product-row col-sm-7\">200\u00b5l<\/div>\n<\/div>\n<\/td>\n<\/tr>\n<\/tfoot>\n<\/table>\n<\/div>\n<p>[\/et_pb_text][\/et_pb_column][et_pb_column type=&#8221;1_4&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.22.2&#8243; _module_preset=&#8221;default&#8221; locked=&#8221;off&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<div class=\"o\">\n<p><span style=\"color: #ff6600; font-size: 14px;\"><strong><a style=\"color: #ff6600;\" href=\"\/?page_id=16282\">ViralEntry\u2122 Transduction Enhancer (100X)<\/a><\/strong><\/span><\/p>\n<hr \/>\n<table style=\"border: none; border-color: #ef6331;\">\n<thead>\n<tr style=\"border-color: #ef6331;\">\n<th style=\"text-align: center; border-color: #ef6331;\">\n<div class=\"col-xs-5 col-md-3 abm-text-bold\"><strong><span style=\"color: #000000;\">Cat. No.<\/span><\/strong><\/div>\n<\/th>\n<th>\n<div class=\"abm-product-more-line\">\n<div class=\"abm-product-row col-sm-7\">\n<div class=\"abm-product-row col-sm-7\">G515<\/div>\n<\/div>\n<\/div>\n<\/th>\n<\/tr>\n<\/thead>\n<tfoot>\n<tr style=\"border-color: #ef6331;\">\n<td style=\"text-align: center;\"><strong><span style=\"color: #000000;\">Unit<\/span><\/strong><\/td>\n<td>\n<div class=\"abm-product-more-line\">\n<div class=\"abm-product-row col-sm-7\">1.0 ml<\/div>\n<\/div>\n<\/td>\n<\/tr>\n<\/tfoot>\n<\/table>\n<\/div>\n<p>[\/et_pb_text][\/et_pb_column][et_pb_column type=&#8221;1_4&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.22.2&#8243; _module_preset=&#8221;default&#8221; locked=&#8221;off&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<div class=\"o\">\n<p><span style=\"color: #ff6600; font-size: 15px;\"><strong><a style=\"color: #ff6600;\" href=\"\/?page_id=6336\">Lentivirus Promoter Blast\u2122 kit<\/a><\/strong><\/span><\/p>\n<hr \/>\n<table style=\"border: none; border-color: #ef6331;\">\n<thead>\n<tr style=\"border-color: #ef6331;\">\n<th style=\"text-align: center; border-color: #ef6331;\">\n<div class=\"col-xs-5 col-md-3 abm-text-bold\"><strong><span style=\"color: #000000;\">Cat. No.<\/span><\/strong><\/div>\n<\/th>\n<th>\n<div class=\"abm-product-row col-sm-7\">\n<div class=\"abm-product-row col-sm-7\">LV950<\/div>\n<\/div>\n<\/th>\n<\/tr>\n<\/thead>\n<tfoot>\n<tr style=\"border-color: #ef6331;\">\n<td style=\"text-align: center;\"><strong><span style=\"color: #000000;\">Unit<\/span><\/strong><\/td>\n<td>\n<div class=\"abm-product-more-line\">\n<div class=\"abm-product-row col-sm-7\">4 x 1ml<\/div>\n<\/div>\n<\/td>\n<\/tr>\n<\/tfoot>\n<\/table>\n<\/div>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=&#8221;1&#8243; admin_label=&#8221;\uc808&#8221; _builder_version=&#8221;4.23&#8243; _module_preset=&#8221;default&#8221; locked=&#8221;off&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_row _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; custom_margin=&#8221;||-50px||false|false&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;4_4&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.23&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<hr class=\"abm-custom-page-hr\" style=\"width: 100%; height: 4px; background-color: #ef6331;\" \/>\n<p>&nbsp;<\/p>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=&#8221;1_3,1_3,1_3&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;1_3&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<style>\n a:hover  {\ncolor:#ef6331;\n}\na{color:black}\n<\/style>\n<p><strong><a class=\"abm-link\" style=\"color: #ef6331;\" href=\"\/?page_id=373\">General Materials &amp; Others<\/a><\/strong><\/p>\n<p><a href=\"\/?page_id=14770\">PCR, qPCR, and RT<\/a><\/p>\n<p><a href=\"\/?page_id=5926\">PCR Polymerases<\/a><\/p>\n<p><a href=\"\/?page_id=14837\">Molecular Biology Enzymes and Kits<\/a><\/p>\n<p><a href=\"\/?page_id=5908\">SafeView DNA Stains<\/a><\/p>\n<p><a href=\"\/?page_id=6125\">RNA Tracking (RNA Mango)<\/a><\/p>\n<p><a href=\"\/?page_id=14879\">Gels, Blots &amp; ELISAs<\/a><\/p>\n<p><a href=\"\/?page_id=14912\">DNA &amp; RNA Purification Kits<\/a><\/p>\n<p><a href=\"\/?page_id=14940\">Expression Systems<\/a><\/p>\n<p><a href=\"\/?page_id=14989\">Cloning Vectors &amp; Packaging Mixes<\/a><\/p>\n<p><a href=\"\/?page_id=15031\">Reagents and Chemicals<\/a><\/p>\n<p><a href=\"\/?page_id=15046\">Equipment &amp; Lab Consumables<\/a>[\/et_pb_text][et_pb_text _builder_version=&#8221;4.23&#8243; _module_preset=&#8221;default&#8221; hover_enabled=&#8221;0&#8243; global_colors_info=&#8221;{}&#8221; sticky_enabled=&#8221;0&#8243;]<\/p>\n<p><strong><a class=\"abm-link\" style=\"color: #ef6331;\" href=\"\/?page_id=17100\">Antibodies<\/a><\/strong><\/p>\n<p><a href=\"\/?page_id=17126\">Tag Antibodies<\/a><\/p>\n<p><a href=\"\/?page_id=17139\">Loading Control Antibodies<\/a><\/p>\n<p><a href=\"\/?page_id=17149\">Secondary Antibodies<\/a><\/p>\n<p><a href=\"\/?page_id=17156\">IHC Antibodies<\/a><\/p>\n<p><a href=\"\/?page_id=17168\">Monoclonal Antibodies<\/a><\/p>\n<p><a href=\"\/?page_id=17178\">Polyclonal Antibodies<\/a><\/p>\n<p>[\/et_pb_text][\/et_pb_column][et_pb_column type=&#8221;1_3&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.23&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p><strong><a class=\"abm-link\" style=\"color: #ef6331;\" href=\"\/?page_id=16969\">Cellular Materials<\/a><\/strong><\/p>\n<p><a href=\"\/?page_id=17002\">Immortalized Cell Lines<\/a><\/p>\n<p><a href=\"\/?page_id=17011\">Stable Cell Lines<\/a><\/p>\n<p><a href=\"\/?page_id=17023\">Tumor Cell Lines<\/a><\/p>\n<p><a href=\"\/?page_id=17030\">Primary Cells<\/a><\/p>\n<p><a href=\"\/?page_id=17036\">Media, Reagents, Kits &amp; Plastics<\/a><\/p>\n<p><a href=\"\/?page_id=17045\">Viruses for Cell Immortalization<\/a><\/p>\n<p><a href=\"\/?page_id=17056\">Growth Factors &amp; Cytokines<\/a><\/p>\n<p>[\/et_pb_text][et_pb_text _builder_version=&#8221;4.23&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p><strong><a class=\"abm-link\" style=\"color: #ef6331;\" href=\"https:\/\/www.abmgood.com\/Contract-Research-CRO-Services.html\">Custom Services<\/a><\/strong><\/p>\n<p><a href=\"https:\/\/www.abmgood.com\/orderSupport\">How to Order<\/a><\/p>\n<p><a href=\"https:\/\/www.abmgood.com\/technical\">Tech Support<\/a><\/p>\n<p><a href=\"https:\/\/www.abmgood.com\/research\">Research Resources<\/a><\/p>\n<p>[\/et_pb_text][\/et_pb_column][et_pb_column type=&#8221;1_3&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.23&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p><strong><a class=\"abm-link\" style=\"color: #ef6331;\" href=\"https:\/\/www.abmgood.com\/genetic-materials.html\">Genetic Materials<\/a><\/strong><\/p>\n<p><a href=\"\/?page_id=382\">Proteins<\/a><\/p>\n<p><a href=\"\/?page_id=16655\">Lentivirus<\/a><\/p>\n<p><a href=\"https:\/\/www.abmgood.com\/AAV-Adeno-Associated-Virus.html\">AAV<\/a><\/p>\n<p><a href=\"\/?page_id=16688\">CRISPR<\/a><\/p>\n<p><a href=\"\/?page_id=16744\">siRNA<\/a><\/p>\n<p><a href=\"\/?page_id=16775\">miRNA and 3&#8217;UTR<\/a><\/p>\n<p><a href=\"\/?page_id=16796\">Control Vectors &amp; Viruses<\/a><\/p>\n<p><a href=\"\/?page_id=16808\">Adenovirus<\/a><\/p>\n<p><a href=\"\/?page_id=16815\">ORF Vectors<\/a><\/p>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Speedy Lentivirus Purification HOME abm Cat. No. LV999 Unit 100 ml <style id=\"sp_tab_dynamic_style16361\">#sp-tabpro-wrapper_16361.sp-tab__lay-default .sp-tab__tab-content {width: 100%;}#sp-tabpro-wrapper_16361 .animated {-webkit-animation-duration: 500ms;animation-duration: 500ms;}#sp-wp-tabs-wrapper_16361 .sp-tab__nav {justify-content: start;}#sp-wp-tabs-wrapper_16361.sp-tab__lay-default.sp-tab__horizontal-bottom {display: flex;flex-direction: column-reverse;}#sp-wp-tabs-wrapper_16361.sp-tab__lay-default.sp-tab__horizontal-bottom > ul {border-top: 1px solid #f2f2f2;border-bottom: 0;margin-top: 0;}#sp-wp-tabs-wrapper_16361.sp-tab__lay-default.sp-tab__horizontal-bottom > ul > li label.sp-tab__active {border-color: transparent #f2f2f2#f2f2f2;margin-top: -1px;}#sp-wp-tabs-wrapper_16361.sp-tab__lay-default.sp-tab__horizontal-bottom > ul > li label,#sp-wp-tabs-wrapper_16361.sp-tab__lay-default.sp-tab__horizontal-bottom > ul > li a,#sp-wp-tabs-wrapper_16361.sp-tab__lay-default.sp-tab__horizontal-bottom > ul > .sp-tab__nav-item 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#444;}#sp-wp-tabs-wrapper_16361.sp-tab__lay-default > .sp-tab__tab-content .sp-tab__tab-pane ul li a, #sp-wp-tabs-wrapper_16361.sp-tab__lay-default .sp-tab__tab-content .sp-tab__tab-pane ol li a {color: #444;}<\/style>\t\t<div id=\"sp-wp-tabs-wrapper_16361\" class=\"sp-tab__lay-default\" data-preloader=\"1\" data-activemode=\"tabs-activator-event-click\">\n\t\t\t<div class=\"sp-tab__preloader\">\n\t\t<div class=\"sp-tab__spinner\">\n\t\t\t<div class=\"rect1\"><\/div>\n\t\t\t<div class=\"rect2\"><\/div>\n\t\t\t<div class=\"rect3\"><\/div>\n\t\t\t<div class=\"rect4\"><\/div>\n\t\t\t<div class=\"rect5\"><\/div>\n\t\t<\/div>\n\t<\/div>\n\t<ul class=\"sp-tab__nav sp-tab__nav-tabs\" id=\"sp-tab__ul\" role=\"tablist\">\n\t\t\t\t<li class=\"sp-tab__nav-item\" role=\"tab\">\n\t\t\t\t<label class=\"sp-tab__nav-link sp-tab__active\" data-sptoggle=\"tab\" for=\"#tab-163611\" role=\"tab\" aria-controls=tab-163611 aria-selected=true tabindex=0>\n\t\t\t\t\t<span class=\"tab_title_area\"><H3 class=\"sp-tab__tab_title\">Specifications<\/H3><\/span>\n\t\t\t\t<\/label>\n\t\t\t<\/li>\n\t\t\t\t\t\t<li class=\"sp-tab__nav-item\" role=\"tab\">\n\t\t\t\t<label class=\"sp-tab__nav-link\" data-sptoggle=\"tab\" for=\"#tab-163612\" role=\"tab\" aria-controls=tab-163612 aria-selected=true tabindex=0>\n\t\t\t\t\t<span class=\"tab_title_area\"><H3 class=\"sp-tab__tab_title\">Datasheet<\/H3><\/span>\n\t\t\t\t<\/label>\n\t\t\t<\/li>\n\t\t\t\t\t\t<li class=\"sp-tab__nav-item\" role=\"tab\">\n\t\t\t\t<label class=\"sp-tab__nav-link\" data-sptoggle=\"tab\" for=\"#tab-163613\" role=\"tab\" aria-controls=tab-163613 aria-selected=true tabindex=0>\n\t\t\t\t\t<span class=\"tab_title_area\"><H3 class=\"sp-tab__tab_title\">Documents<\/H3><\/span>\n\t\t\t\t<\/label>\n\t\t\t<\/li>\n\t\t\t\t\t\t<li class=\"sp-tab__nav-item\" role=\"tab\">\n\t\t\t\t<label class=\"sp-tab__nav-link\" data-sptoggle=\"tab\" for=\"#tab-163614\" role=\"tab\" aria-controls=tab-163614 aria-selected=true tabindex=0>\n\t\t\t\t\t<span class=\"tab_title_area\"><H3 class=\"sp-tab__tab_title\">FAQs<\/H3><\/span>\n\t\t\t\t<\/label>\n\t\t\t<\/li>\n\t\t\t\t\t\t<li class=\"sp-tab__nav-item\" role=\"tab\">\n\t\t\t\t<label class=\"sp-tab__nav-link\" data-sptoggle=\"tab\" for=\"#tab-163615\" role=\"tab\" aria-controls=tab-163615 aria-selected=true tabindex=0>\n\t\t\t\t\t<span class=\"tab_title_area\"><H3 class=\"sp-tab__tab_title\">References<\/H3><\/span>\n\t\t\t\t<\/label>\n\t\t\t<\/li>\n\t\t\t\t\t\t<li class=\"sp-tab__nav-item\" role=\"tab\">\n\t\t\t\t<label class=\"sp-tab__nav-link\" data-sptoggle=\"tab\" for=\"#tab-163616\" role=\"tab\" aria-controls=tab-163616 aria-selected=true tabindex=0>\n\t\t\t\t\t<span class=\"tab_title_area\"><H3 class=\"sp-tab__tab_title\">Reviews<\/H3><\/span>\n\t\t\t\t<\/label>\n\t\t\t<\/li>\n\t\t\t<\/ul>\n<div class=\"sp-tab__tab-content\">\n\t\t\t\t<div id=\"tab-163611\" class=\"sp-tab__tab-pane sp-tab__show sp-tab__active\" role=\"tabpanel\">\n\t\t\t\t\t<div class=\"sp-tab-content animated fadeIn\"><table>\n<tbody>\n<tr class=\"abm-specifications-cat_no\">\n<td class=\"abm-specifications-label\" valign=\"top\">Cat. No.<\/td>\n<td class=\"abm-specifications-value\" valign=\"top\"><span id=\"abm-specifications-cat_no\">LV999<\/span><\/td>\n<\/tr>\n<tr class=\"abm-specifications-name\">\n<td class=\"abm-specifications-label\" valign=\"top\">Name<\/td>\n<td class=\"abm-specifications-value\" valign=\"top\">Speedy Lentivirus Purification<\/td>\n<\/tr>\n<tr class=\"abm-specifications-unit_quantity\">\n<td class=\"abm-specifications-label\" valign=\"top\">Unit<\/td>\n<td class=\"abm-specifications-value\" valign=\"top\">100 ml<\/td>\n<\/tr>\n<tr class=\"abm-specifications-description\">\n<td class=\"abm-specifications-label\" valign=\"top\">Description<\/td>\n<td class=\"abm-specifications-value\" valign=\"top\">Recombinant lentiviral vectors have been shown to be a powerful tool for stable gene transfer to both dividing and non-dividing cells in vitro and in vivo.Through years of experience with lentiviral vectors, ABM has developed its own proprietary pLenti-combo packaging mix and an efficient protocol for rapid production of recombinant lentiviral vectors with titers up to 10<sup>7<\/sup>IU\/ml. The quickest and easiest way to purify and concentrate lentiviral particles up to 100x.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<div id=\"tab-163612\" class=\"sp-tab__tab-pane \" role=\"tabpanel\">\n\t\t\t\t\t<div class=\"sp-tab-content animated fadeIn\"><div class=\"abm-doc-title\">Datasheet<\/div>\n<ul class=\"abm-document-list\">\n<li><span style=\"color: #ff6600\"><a style=\"color: #ff6600\" href=\"https:\/\/www.abmgood.com\/uploads\/document\/lv999-datasheet%202023.pdf\" target=\"_blank\" rel=\"noopener\">Speedy Lentivirus Purification LV999 Datasheet<\/a><\/span><\/li>\n<\/ul>\n<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<div id=\"tab-163613\" class=\"sp-tab__tab-pane \" role=\"tabpanel\">\n\t\t\t\t\t<div class=\"sp-tab-content animated fadeIn\"><div class=\"abm-doc-div\">\n<div class=\"abm-doc-title\">Search CoA\u00a0<a class=\"abm-link\" href=\"https:\/\/www.abmgood.com\/CoA-Library.html\" target=\"_blank\" rel=\"noopener\">here<\/a><\/div>\n<\/div>\n<div class=\"abm-doc-div\">\n<div class=\"abm-doc-title\">Supporting Protocol<\/div>\n<ul class=\"abm-document-list\" data-count=\"1\">\n<li><a href=\"https:\/\/www.abmgood.com\/document\/Drug%20selection%20killing%20curve%20Final_111315.pdf\" target=\"_blank\" rel=\"noopener\"><span style=\"color: #ff6600\">Selection-Drug Killing Curve<\/span><\/a><\/li>\n<li><span style=\"color: #ff6600\"><a style=\"color: #ff6600\" href=\"https:\/\/www.abmgood.com\/uploads\/document\/A4_lenti_packaging_2023_V2.pdf\" target=\"_blank\" rel=\"noopener\">Lentivector Packaging Protocol<\/a><\/span><\/li>\n<li><span style=\"color: #ff6600\"><a style=\"color: #ff6600\" href=\"https:\/\/www.abmgood.com\/uploads\/document\/a4_lenti_infection-with_spinoculation_.pdf\" target=\"_blank\" rel=\"noopener\">Lentivirus Infection Protocol<\/a><\/span><\/li>\n<li><span style=\"color: #ff6600\"><a style=\"color: #ff6600\" href=\"https:\/\/www.abmgood.com\/document\/LV999-Protocol_V1.pdf\" target=\"_blank\" rel=\"noopener\">Speedy Lentivirus Purification Protocol<\/a><\/span><\/li>\n<\/ul>\n<\/div>\n<div class=\"abm-doc-div\">\n<div class=\"abm-doc-title\">Other<\/div>\n<ul class=\"abm-document-list\" data-count=\"1\">\n<li><span style=\"color: #ff6600\"><a style=\"color: #ff6600\" href=\"https:\/\/www.abmgood.com\/uploads\/document\/Lentivirus%20incompetency-abm.pdf\" target=\"_blank\" rel=\"noopener\">Enhanced Lentivirus Safety Features: Replication Incompetency<\/a><\/span><\/li>\n<li><span style=\"color: #ff6600\"><a style=\"color: #ff6600\" href=\"https:\/\/www.abmgood.com\/uploads\/document\/A4%20%20%20lenti%20infection%20%20Cancer%20Cell%20Line%20Guide.pdf\" target=\"_blank\" rel=\"noopener\">Suggested MOI for Common Cancer Cell Lines<\/a><\/span><\/li>\n<li><span style=\"color: #ff6600\"><a style=\"color: #ff6600\" href=\"https:\/\/www.abmgood.com\/document\/\/l\/e\/lentiviral-system-faq.pdf\" target=\"_blank\" rel=\"noopener\">Lentiviral System FAQ<\/a><\/span><\/li>\n<\/ul>\n<\/div>\n<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<div id=\"tab-163614\" class=\"sp-tab__tab-pane \" role=\"tabpanel\">\n\t\t\t\t\t<div class=\"sp-tab-content animated fadeIn\"><div class=\"product-info-box abm-mt-5 abm-pt-5\">\n<div class=\"tab-content\">\n<div id=\"faqs\" class=\"tab-pane active\" role=\"tabpanel\">\n<div class=\"abm-doc-div\"><style>#sp-ea-16362 .spcollapsing { height: 0; overflow: hidden; transition-property: height;transition-duration: 500ms;}#sp-ea-16362.sp-easy-accordion>.sp-ea-single {border: 1px solid #e2e2e2; }#sp-ea-16362.sp-easy-accordion>.sp-ea-single>.ea-header a {color: #444;}#sp-ea-16362.sp-easy-accordion>.sp-ea-single>.sp-collapse>.ea-body {background: #fff; color: #444;}#sp-ea-16362.sp-easy-accordion>.sp-ea-single {background: #eee;}#sp-ea-16362.sp-easy-accordion>.sp-ea-single>.ea-header a .ea-expand-icon.fa { float: left; color: #444;font-size: 16px;}<\/style><div id=\"sp_easy_accordion-1782566630\"><div id=\"sp-ea-16362\" class=\"sp-ea-one sp-easy-accordion\" data-ex-icon=\"fa-minus\" data-col-icon=\"fa-plus\"  data-ea-active=\"ea-click\"  data-ea-mode=\"vertical\" data-preloader=\"\" data-scroll-active-item=\"\" data-offset-to-scroll=\"0\"><div class=\"ea-card ea-expand sp-ea-single\"><h3 class=\"ea-header\"><a class=\"collapsed\" data-sptoggle=\"spcollapse\" data-sptarget=#collapse163620 href=\"javascript:void(0)\"  aria-expanded=\"true\"><i class=\"ea-expand-icon fa fa-minus\"><\/i> What is the mechanism of purification for the Speedy Lentivirus Purification?<\/a><\/h3><div class=\"sp-collapse spcollapse collapsed show\" id=\"collapse163620\" data-parent=#sp-ea-16362><div class=\"ea-body\"><p>The Speedy Lentivirus Purification is a positively charged matrix solution that binds to the negatively charged lentivirus, forming a precipitation that can be pelleted through centrifugation.<\/p>\n<\/div><\/div><\/div><div class=\"ea-card  sp-ea-single\"><h3 class=\"ea-header\"><a class=\"collapsed\" data-sptoggle=\"spcollapse\" data-sptarget=#collapse163621 href=\"javascript:void(0)\"  aria-expanded=\"false\"><i class=\"ea-expand-icon fa fa-plus\"><\/i> Can it also be used for Retrovirus purification?<\/a><\/h3><div class=\"sp-collapse spcollapse \" id=\"collapse163621\" data-parent=#sp-ea-16362><div class=\"ea-body\"><p>No, because retroviruses are too sensitive to survive the required centrifugation step.<\/p>\n<\/div><\/div><\/div><div class=\"ea-card  sp-ea-single\"><h3 class=\"ea-header\"><a class=\"collapsed\" data-sptoggle=\"spcollapse\" data-sptarget=#collapse163622 href=\"javascript:void(0)\"  aria-expanded=\"false\"><i class=\"ea-expand-icon fa fa-plus\"><\/i> Will the lack of FBS and calcium affect the functionality of the speedy lentivirus purification?<\/a><\/h3><div class=\"sp-collapse spcollapse \" id=\"collapse163622\" data-parent=#sp-ea-16362><div class=\"ea-body\"><p>No, the function will not be affected.<\/p>\n<\/div><\/div><\/div><div class=\"ea-card  sp-ea-single\"><h3 class=\"ea-header\"><a class=\"collapsed\" data-sptoggle=\"spcollapse\" data-sptarget=#collapse163623 href=\"javascript:void(0)\"  aria-expanded=\"false\"><i class=\"ea-expand-icon fa fa-plus\"><\/i> What buffer should I use for resuspension?<\/a><\/h3><div class=\"sp-collapse spcollapse \" id=\"collapse163623\" data-parent=#sp-ea-16362><div class=\"ea-body\"><p>The pelleted lentivirus can be resuspended in any appropriate buffer.<\/p>\n<\/div><\/div><\/div><div class=\"ea-card  sp-ea-single\"><h3 class=\"ea-header\"><a class=\"collapsed\" data-sptoggle=\"spcollapse\" data-sptarget=#collapse163624 href=\"javascript:void(0)\"  aria-expanded=\"false\"><i class=\"ea-expand-icon fa fa-plus\"><\/i> Can I remove the matrix as much as possible from the resuspended lentivirus?<\/a><\/h3><div class=\"sp-collapse spcollapse \" id=\"collapse163624\" data-parent=#sp-ea-16362><div class=\"ea-body\"><p>Yes it is possible but the lentivirus should go through a buffer exchange procedure. Our recommended method is loading the resuspended lentivirus onto a 20mL-50uL ultrafiltration unit (30,000 MWCO), add PBS, then wash out the matrix by multiple centrifuge spins and discard the flowthrough. Once the exchange has occurred, use the desired volume of solvent to resuspend the lentivirus from the top filter and aliquot into storage containers.<\/p>\n<\/div><\/div><\/div><div class=\"ea-card  sp-ea-single\"><h3 class=\"ea-header\"><a class=\"collapsed\" data-sptoggle=\"spcollapse\" data-sptarget=#collapse163625 href=\"javascript:void(0)\"  aria-expanded=\"false\"><i class=\"ea-expand-icon fa fa-plus\"><\/i> What is the difference between Retro-, Lenti-, and Adeno- viruses?<\/a><\/h3><div class=\"sp-collapse spcollapse \" id=\"collapse163625\" data-parent=#sp-ea-16362><div class=\"ea-body\"><p>Retrovirus: Classic, can integrate into the genome but with low transduction efficiency. They are useful for gene transfer and protein expression in cells that have low transfection efficiency with other transfection reagents. Lentivirus: Can integrate into the genome with relatively high transduction efficiency and they are very useful for cells that have low transfection efficiency with other transfection reagents. No special competent cells required, as they are stable plasmids. Lentiviruses are a powerful tool for stable gene transfer to both dividing and non-dividing cells in vitro and in vivo. Adenovirus: Only work transiently (about 7 days) but have almost 100% transduction efficiency. Adenoviruses can infect a broad range of cell types with the highest efficiency and infection is not dependent on active host cell division. A second key feature is that high virus titers and high-level gene expression can be obtained in most mammalian cells.<\/p>\n<\/div><\/div><\/div><div class=\"ea-card  sp-ea-single\"><h3 class=\"ea-header\"><a class=\"collapsed\" data-sptoggle=\"spcollapse\" data-sptarget=#collapse163626 href=\"javascript:void(0)\"  aria-expanded=\"false\"><i class=\"ea-expand-icon fa fa-plus\"><\/i> What are the correct concentration units for each recombinant viral particle?<\/a><\/h3><div class=\"sp-collapse spcollapse \" id=\"collapse163626\" data-parent=#sp-ea-16362><div class=\"ea-body\"><p>For lentiviruses and retroviruses, they are measured in CFU\/ml (colony-forming units per millilitre). Transduction with lentiviruses and retroviruses can cause the formation of colonies, which can be quantified for concentration. For AAV the titer is measured as genome copies per mL (GC\/mL). Adenoviruses are measured as PFU\/ml (plaque-forming units per millilitre). Transduction with adenoviruses will kill packaging cells, forming plaques in the process for quantification. The concentration for all three types of viruses can also be classified as IU\/ml (Infectious Units\/ml). Ultimately, the units refers to the viral particles and different units reflect the different assays involved.<\/p>\n<\/div><\/div><\/div><div class=\"ea-card  sp-ea-single\"><h3 class=\"ea-header\"><a class=\"collapsed\" data-sptoggle=\"spcollapse\" data-sptarget=#collapse163627 href=\"javascript:void(0)\"  aria-expanded=\"false\"><i class=\"ea-expand-icon fa fa-plus\"><\/i> What do I use to check if my cells were successfully immortalized by the SV40 agent?<\/a><\/h3><div class=\"sp-collapse spcollapse \" id=\"collapse163627\" data-parent=#sp-ea-16362><div class=\"ea-body\"><p>We have an SV40 T antibody that can be used for the western blot analysis. The catalog number is G202. Otherwise, a qPCR primer can be designed on the SV40 gene for qPCR analysis. The sequence can be found in the link below: http:\/\/www.abmgood.com\/pLenti%20SV40-Vector-Location-Map.html<\/p>\n<\/div><\/div><\/div><div class=\"ea-card  sp-ea-single\"><h3 class=\"ea-header\"><a class=\"collapsed\" data-sptoggle=\"spcollapse\" data-sptarget=#collapse163628 href=\"javascript:void(0)\"  aria-expanded=\"false\"><i class=\"ea-expand-icon fa fa-plus\"><\/i> What filter can I used for filter sterilization after the purification step?<\/a><\/h3><div class=\"sp-collapse spcollapse \" id=\"collapse163628\" data-parent=#sp-ea-16362><div class=\"ea-body\"><p>We recommend a low protein binding filter with membrane pores of 0.2-0.45um. A few suggested membrane materials are PVDF, PES, and Supor (from Pall).<\/p>\n<\/div><\/div><\/div><div class=\"ea-card  sp-ea-single\"><h3 class=\"ea-header\"><a class=\"collapsed\" data-sptoggle=\"spcollapse\" data-sptarget=#collapse163629 href=\"javascript:void(0)\"  aria-expanded=\"false\"><i class=\"ea-expand-icon fa fa-plus\"><\/i> What are the primers to use for SV40 identification?<\/a><\/h3><div class=\"sp-collapse spcollapse \" id=\"collapse163629\" data-parent=#sp-ea-16362><div class=\"ea-body\"><p>SV40 Forward Primer Sequence 5\u2019 ACTGAGGGGCCTGAAATGA SV40 Reverse Primer Sequence 5\u2019 GACTCAGGGCATGAAACAGG These are qPCR primers and the band size is 61 bp.<\/p>\n<\/div><\/div><\/div><div class=\"ea-card  sp-ea-single\"><h3 class=\"ea-header\"><a class=\"collapsed\" data-sptoggle=\"spcollapse\" data-sptarget=#collapse1636210 href=\"javascript:void(0)\"  aria-expanded=\"false\"><i class=\"ea-expand-icon fa fa-plus\"><\/i> What advantages \/ disadvantages exist between the Lenti-SV40, -SV40T, and SV40T+t vectors?<\/a><\/h3><div class=\"sp-collapse spcollapse \" id=\"collapse1636210\" data-parent=#sp-ea-16362><div class=\"ea-body\"><p>There are simply differences in the content of all vectors due to customer demand for variety. Lenti-SV40 will contain the whole SV40 gene, -SV40T, the large T Antigen only, and -SV40T&t the large and small T antigens only. It is up to the end user to decide which vectors will best suit their project, however we have successfully used Lenti-SV40 (whole gene) in a wide range of immortalization projects.<\/p>\n<\/div><\/div><\/div><div class=\"ea-card  sp-ea-single\"><h3 class=\"ea-header\"><a class=\"collapsed\" data-sptoggle=\"spcollapse\" data-sptarget=#collapse1636211 href=\"javascript:void(0)\"  aria-expanded=\"false\"><i class=\"ea-expand-icon fa fa-plus\"><\/i> What is the accession number for the SV40?<\/a><\/h3><div class=\"sp-collapse spcollapse \" id=\"collapse1636211\" data-parent=#sp-ea-16362><div class=\"ea-body\"><p>The SV40 covers the entire genome and the accession number is J02400.1. You can use this information to design primers for conventional PCR as well.<\/p>\n<\/div><\/div><\/div><div class=\"ea-card  sp-ea-single\"><h3 class=\"ea-header\"><a class=\"collapsed\" data-sptoggle=\"spcollapse\" data-sptarget=#collapse1636212 href=\"javascript:void(0)\"  aria-expanded=\"false\"><i class=\"ea-expand-icon fa fa-plus\"><\/i> How long after transduction can the infection efficiency be observed?<\/a><\/h3><div class=\"sp-collapse spcollapse \" id=\"collapse1636212\" data-parent=#sp-ea-16362><div class=\"ea-body\"><p>You can observe transduction efficiency from 48 hours up to 5 days after infection.<\/p>\n<\/div><\/div><\/div><div class=\"ea-card  sp-ea-single\"><h3 class=\"ea-header\"><a class=\"collapsed\" data-sptoggle=\"spcollapse\" data-sptarget=#collapse1636213 href=\"javascript:void(0)\"  aria-expanded=\"false\"><i class=\"ea-expand-icon fa fa-plus\"><\/i> What are the primers to use for SV40T and SV40T tsA58 detection?<\/a><\/h3><div class=\"sp-collapse spcollapse \" id=\"collapse1636213\" data-parent=#sp-ea-16362><div class=\"ea-body\"><p>PCR primers: SV40T Forward Primer Sequence 5\u2019 AGCCTGTAGAACCAAACATT 3' SV40T Reverse Primer Sequence 5\u2019 CTGCTGACTCTCAACATTCT 3' The two primers should amplify the region between 3677-4468bp, giving a 792bp fragment.<\/p>\n<\/div><\/div><\/div><div class=\"ea-card  sp-ea-single\"><h3 class=\"ea-header\"><a class=\"collapsed\" data-sptoggle=\"spcollapse\" data-sptarget=#collapse1636214 href=\"javascript:void(0)\"  aria-expanded=\"false\"><i class=\"ea-expand-icon fa fa-plus\"><\/i>  What is the sequence of the SV40 large T antigen?<\/a><\/h3><div class=\"sp-collapse spcollapse \" id=\"collapse1636214\" data-parent=#sp-ea-16362><div class=\"ea-body\"><p>This information can be accessed on this page by clicking on \"pLenti-SV40-T\" under vector map. The Large T antigen is at position 5079-5927.<\/p>\n<\/div><\/div><\/div><div class=\"ea-card  sp-ea-single\"><h3 class=\"ea-header\"><a class=\"collapsed\" data-sptoggle=\"spcollapse\" data-sptarget=#collapse1636215 href=\"javascript:void(0)\"  aria-expanded=\"false\"><i class=\"ea-expand-icon fa fa-plus\"><\/i> In the final solution what medium and FBS should I use to keep the concentrated viruses?<\/a><\/h3><div class=\"sp-collapse spcollapse \" id=\"collapse1636215\" data-parent=#sp-ea-16362><div class=\"ea-body\"><p>When using our Speedy Lentivirus Purification (Cat#LV999), the choice of which medium and FBS to use to keep the concentrated viruses will vary based upon the intended downstream application of the lentivirus; i.e. depending on the complete media required for your cell line of interest. You can re-suspend the pellet in the complete media of the cell line which will be infected with this virus prep, to avoid dilution. For example, if 293T cells were the intended cell line, we would recommend using DMEM + 10% FBS. If in doubt, serum-free DMEM is typically a safe choice, however the required dilution will be a factor to consider.<\/p>\n<\/div><\/div><\/div><div class=\"ea-card  sp-ea-single\"><h3 class=\"ea-header\"><a class=\"collapsed\" data-sptoggle=\"spcollapse\" data-sptarget=#collapse1636216 href=\"javascript:void(0)\"  aria-expanded=\"false\"><i class=\"ea-expand-icon fa fa-plus\"><\/i> For G221 and LV620, what does the 'V12' in RasV12 mean?<\/a><\/h3><div class=\"sp-collapse spcollapse \" id=\"collapse1636216\" data-parent=#sp-ea-16362><div class=\"ea-body\"><p>The V12 means that amino acid # 12 is mutated from a Valine to a Glycine. Other than that, the sequence matches the coding region of HRAS perfectly (NM_005343).<\/p>\n<\/div><\/div><\/div><div class=\"ea-card  sp-ea-single\"><h3 class=\"ea-header\"><a class=\"collapsed\" data-sptoggle=\"spcollapse\" data-sptarget=#collapse1636217 href=\"javascript:void(0)\"  aria-expanded=\"false\"><i class=\"ea-expand-icon fa fa-plus\"><\/i> Where is the SV40T tsA58 gene sequence?<\/a><\/h3><div class=\"sp-collapse spcollapse \" id=\"collapse1636217\" data-parent=#sp-ea-16362><div class=\"ea-body\"><p>The SV40T tsA58 gene is located between 3138-5264bp, with the Alanine-to-Valine mutation at amino acid 438.<\/p>\n<\/div><\/div><\/div><\/div><\/div><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<div id=\"tab-163615\" class=\"sp-tab__tab-pane \" role=\"tabpanel\">\n\t\t\t\t\t<div class=\"sp-tab-content animated fadeIn\"><div class=\"product-info-box abm-mt-5 abm-pt-5\">\n<div class=\"tab-content\">\n<div id=\"references\" class=\"tab-pane active\" role=\"tabpanel\">\n<div>\n<div class=\"abm-doc-div\">There are no references for this product yet!<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"abm-options-product\">\n<div class=\"abm-clear-div\"><\/div>\n<div class=\"col-sm-12 col-md-12 col-lg-12\"><\/div>\n<\/div>\n<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<div id=\"tab-163616\" class=\"sp-tab__tab-pane \" role=\"tabpanel\">\n\t\t\t\t\t<div class=\"sp-tab-content animated fadeIn\"><p>This product has no review yet.<\/p>\n<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<\/div>\n\t\tControls and Related Product:\u00a0 DNAfectin\u2122 Plus Transfection Reagent Cat. No. G2500 Unit 1.0 ml 2nd Generation Packaging System Mix Cat. No. LV003 Unit 200\u00b5l qPCR Lentivirus Titer Kit Cat. No. LV900 Unit 100 rxn 3rd Generation Packaging System Mix Cat. No. LV053 Unit [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"_links":{"self":[{"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=\/wp\/v2\/pages\/16357"}],"collection":[{"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=16357"}],"version-history":[{"count":5,"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=\/wp\/v2\/pages\/16357\/revisions"}],"predecessor-version":[{"id":17384,"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=\/wp\/v2\/pages\/16357\/revisions\/17384"}],"wp:attachment":[{"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=16357"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}