{"id":13598,"date":"2021-06-01T13:41:08","date_gmt":"2021-06-01T04:41:08","guid":{"rendered":"http:\/\/itsbio.co.kr\/?page_id=13598"},"modified":"2021-06-08T09:25:24","modified_gmt":"2021-06-08T00:25:24","slug":"ez-ldh","status":"publish","type":"page","link":"http:\/\/itsbio.co.kr\/?page_id=13598","title":{"rendered":"EZ-LDH"},"content":{"rendered":"

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HOME<\/a> \"\" DoGen<\/a> \"\"Cell Based Assay<\/a><\/p>\n<\/div>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.4.2″ vertical_offset_tablet=”0″ horizontal_offset_tablet=”0″ z_index_tablet=”0″ custom_css_main_element=”padding-top:0;||padding-bottom:0;||marign-top:0;||marign-bottom:0;” box_shadow_horizontal_tablet=”0px” box_shadow_vertical_tablet=”0px” box_shadow_blur_tablet=”40px” box_shadow_spread_tablet=”0px”][et_pb_column type=”4_4″ _builder_version=”3.0.47″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_image src=”http:\/\/itsbio.co.kr\/wp-content\/uploads\/2021\/06\/DoGen-logo.png” title_text=”DoGen logo” _builder_version=”4.6.3″ vertical_offset_tablet=”0″ horizontal_offset_tablet=”0″ width=”8%” z_index_tablet=”0″ box_shadow_horizontal_tablet=”0px” box_shadow_vertical_tablet=”0px” box_shadow_blur_tablet=”40px” box_shadow_spread_tablet=”0px”][\/et_pb_image][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.4.2″ vertical_offset_tablet=”0″ horizontal_offset_tablet=”0″ custom_margin=”1px|||||” z_index_tablet=”0″ border_width_bottom=”5px” box_shadow_horizontal_tablet=”0px” box_shadow_vertical_tablet=”0px” box_shadow_blur_tablet=”40px” box_shadow_spread_tablet=”0px”][et_pb_row column_structure=”1_2,1_2″ _builder_version=”3.25″ background_size=”initial” background_position=”top_left” background_repeat=”repeat”][et_pb_column type=”1_2″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_image src=”http:\/\/itsbio.co.kr\/wp-content\/uploads\/2021\/06\/EZ-LDH.png” title_text=”EZ-LDH” align=”center” admin_label=”\uc774\ubbf8\uc9c0” _builder_version=”4.6.3″ vertical_offset_tablet=”0″ horizontal_offset_tablet=”0″ width=”50%” max_width=”100%” custom_padding=”0px||0px|||” z_index_tablet=”0″ box_shadow_horizontal_tablet=”0px” box_shadow_vertical_tablet=”0px” box_shadow_blur_tablet=”40px” box_shadow_spread_tablet=”0px”][\/et_pb_image][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.6.3″ vertical_offset_tablet=”0″ horizontal_offset_tablet=”0″ custom_margin=”||58px|||” z_index_tablet=”0″ text_text_shadow_horizontal_length_tablet=”0px” text_text_shadow_vertical_length_tablet=”0px” text_text_shadow_blur_strength_tablet=”1px” link_text_shadow_horizontal_length_tablet=”0px” link_text_shadow_vertical_length_tablet=”0px” link_text_shadow_blur_strength_tablet=”1px” ul_text_shadow_horizontal_length_tablet=”0px” ul_text_shadow_vertical_length_tablet=”0px” ul_text_shadow_blur_strength_tablet=”1px” ol_text_shadow_horizontal_length_tablet=”0px” ol_text_shadow_vertical_length_tablet=”0px” ol_text_shadow_blur_strength_tablet=”1px” quote_text_shadow_horizontal_length_tablet=”0px” quote_text_shadow_vertical_length_tablet=”0px” quote_text_shadow_blur_strength_tablet=”1px” header_text_shadow_horizontal_length_tablet=”0px” header_text_shadow_vertical_length_tablet=”0px” header_text_shadow_blur_strength_tablet=”1px” header_2_text_shadow_horizontal_length_tablet=”0px” header_2_text_shadow_vertical_length_tablet=”0px” header_2_text_shadow_blur_strength_tablet=”1px” header_3_text_shadow_horizontal_length_tablet=”0px” header_3_text_shadow_vertical_length_tablet=”0px” header_3_text_shadow_blur_strength_tablet=”1px” header_4_text_shadow_horizontal_length_tablet=”0px” header_4_text_shadow_vertical_length_tablet=”0px” header_4_text_shadow_blur_strength_tablet=”1px” header_5_text_shadow_horizontal_length_tablet=”0px” header_5_text_shadow_vertical_length_tablet=”0px” header_5_text_shadow_blur_strength_tablet=”1px” header_6_text_shadow_horizontal_length_tablet=”0px” header_6_text_shadow_vertical_length_tablet=”0px” header_6_text_shadow_blur_strength_tablet=”1px” box_shadow_horizontal_tablet=”0px” box_shadow_vertical_tablet=”0px” box_shadow_blur_tablet=”40px” box_shadow_spread_tablet=”0px”]<\/p>\n

EZ-LDH<\/strong><\/span><\/h3>\n

 <\/p>\n

Cell cytotoxicity assay kit<\/span><\/p>\n

[\/et_pb_text][et_pb_text _builder_version=”4.6.3″ vertical_offset_tablet=”0″ horizontal_offset_tablet=”0″ z_index_tablet=”0″ text_text_shadow_horizontal_length_tablet=”0px” text_text_shadow_vertical_length_tablet=”0px” text_text_shadow_blur_strength_tablet=”1px” link_text_shadow_horizontal_length_tablet=”0px” link_text_shadow_vertical_length_tablet=”0px” link_text_shadow_blur_strength_tablet=”1px” ul_text_shadow_horizontal_length_tablet=”0px” ul_text_shadow_vertical_length_tablet=”0px” ul_text_shadow_blur_strength_tablet=”1px” ol_text_shadow_horizontal_length_tablet=”0px” ol_text_shadow_vertical_length_tablet=”0px” ol_text_shadow_blur_strength_tablet=”1px” quote_text_shadow_horizontal_length_tablet=”0px” quote_text_shadow_vertical_length_tablet=”0px” quote_text_shadow_blur_strength_tablet=”1px” header_text_shadow_horizontal_length_tablet=”0px” header_text_shadow_vertical_length_tablet=”0px” header_text_shadow_blur_strength_tablet=”1px” header_2_text_shadow_horizontal_length_tablet=”0px” header_2_text_shadow_vertical_length_tablet=”0px” header_2_text_shadow_blur_strength_tablet=”1px” header_3_text_shadow_horizontal_length_tablet=”0px” header_3_text_shadow_vertical_length_tablet=”0px” header_3_text_shadow_blur_strength_tablet=”1px” header_4_text_shadow_horizontal_length_tablet=”0px” header_4_text_shadow_vertical_length_tablet=”0px” header_4_text_shadow_blur_strength_tablet=”1px” header_5_text_shadow_horizontal_length_tablet=”0px” header_5_text_shadow_vertical_length_tablet=”0px” header_5_text_shadow_blur_strength_tablet=”1px” header_6_text_shadow_horizontal_length_tablet=”0px” header_6_text_shadow_vertical_length_tablet=”0px” header_6_text_shadow_blur_strength_tablet=”1px” box_shadow_horizontal_tablet=”0px” box_shadow_vertical_tablet=”0px” box_shadow_blur_tablet=”40px” box_shadow_spread_tablet=”0px” inline_fonts=”Abel”]<\/p>\n\n\n\n\n\n
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Catalog No.<\/strong><\/span><\/p>\n<\/th>\n

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Assay<\/strong><\/span><\/p>\n<\/th>\n<\/tr>\n

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DG-LDH500<\/span><\/p>\n<\/td>\n

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500 tests<\/span><\/p>\n<\/td>\n<\/tr>\n

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DG-LDH1000<\/span><\/p>\n<\/td>\n

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1000 tests<\/span><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”3.25″ background_size=”initial” background_position=”top_left” background_repeat=”repeat”][et_pb_column type=”4_4″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.6.3″ vertical_offset_tablet=”0″ horizontal_offset_tablet=”0″ z_index_tablet=”0″ text_text_shadow_horizontal_length_tablet=”0px” text_text_shadow_vertical_length_tablet=”0px” text_text_shadow_blur_strength_tablet=”1px” link_text_shadow_horizontal_length_tablet=”0px” link_text_shadow_vertical_length_tablet=”0px” link_text_shadow_blur_strength_tablet=”1px” ul_text_shadow_horizontal_length_tablet=”0px” ul_text_shadow_vertical_length_tablet=”0px” ul_text_shadow_blur_strength_tablet=”1px” ol_text_shadow_horizontal_length_tablet=”0px” ol_text_shadow_vertical_length_tablet=”0px” ol_text_shadow_blur_strength_tablet=”1px” quote_text_shadow_horizontal_length_tablet=”0px” quote_text_shadow_vertical_length_tablet=”0px” quote_text_shadow_blur_strength_tablet=”1px” header_text_shadow_horizontal_length_tablet=”0px” header_text_shadow_vertical_length_tablet=”0px” header_text_shadow_blur_strength_tablet=”1px” header_2_text_shadow_horizontal_length_tablet=”0px” header_2_text_shadow_vertical_length_tablet=”0px” header_2_text_shadow_blur_strength_tablet=”1px” header_3_text_shadow_horizontal_length_tablet=”0px” header_3_text_shadow_vertical_length_tablet=”0px” header_3_text_shadow_blur_strength_tablet=”1px” header_4_text_shadow_horizontal_length_tablet=”0px” header_4_text_shadow_vertical_length_tablet=”0px” header_4_text_shadow_blur_strength_tablet=”1px” header_5_text_shadow_horizontal_length_tablet=”0px” header_5_text_shadow_vertical_length_tablet=”0px” header_5_text_shadow_blur_strength_tablet=”1px” header_6_text_shadow_horizontal_length_tablet=”0px” header_6_text_shadow_vertical_length_tablet=”0px” header_6_text_shadow_blur_strength_tablet=”1px” box_shadow_horizontal_tablet=”0px” box_shadow_vertical_tablet=”0px” box_shadow_blur_tablet=”40px” box_shadow_spread_tablet=”0px”]<\/p>\n

Description<\/strong><\/span><\/p>\n

 <\/p>\n

EZ-LDH \ub294 WST(water soluble tetrazolium salt)\ub97c \uc0ac\uc6a9\ud558\uc5ec \u00a0apoptosis \ud639\uc740 necrosis\ub4f1\uc5d0 \uc758\ud558\uc5ec \uc8fd\uac70\ub098 \uc190\uc0c1\ub41c \uc138\ud3ec\uc5d0\uc11c \ubc29\ucd9c\ub418\ub294 Lactate dehydrogenase(LDH)\uc758 \uc591\uc744 \uace0\uac10\ub3c4\ub85c \uce21\uc815\ud568\uc73c\ub85c\uc368 cytotoxicity\/cytolysis\ub97c \uac04\ub2e8\ud558\uace0 \uc815\ud655\ud558\uac8c \uce21\uc815\ud560 \uc218 \uc788\ub294 kit \uc785\ub2c8\ub2e4. <\/span><\/p>\n

Cytotoxicity (Cell death)\ub294 cell viability test\ub97c \ud1b5\ud558\uc5ec \uac04\uc811\uc801\uc73c\ub85c \uce21\uc815\ud560 \uc218 \uc788\uc9c0\ub9cc, \ub354 sensitive\ud558\uace0, \uc815\ud655\ud55c \uce21\uc815\uc744 \uc704\ud574\uc11c\ub294 cell death \ub610\ub294 cell damage\uc640 \uad00\ub828\ub41c \ud6a8\uc18c\ub97c \uc774\uc6a9\ud55c \ubc29\ubc95\uc73c\ub85c \uc2e4\ud5d8\ud558\ub294 \uac83\uc774 \uc88b\uc2b5\ub2c8\ub2e4.\u00a0<\/span><\/p>\n

Lactate dehydrogenase(LDH)\ub294 \uc138\ud3ec\uc9c8\uc5d0 \uc874\uc7ac\ud558\ub294 stable enzyme\uc73c\ub85c \ud1b5\uc0c1\uc801\uc73c\ub85c\ub294 \uc138\ud3ec\ub9c9\uc744 \ud1b5\uacfc\ud558\uc9c0 \ubabb\ud558\uc5ec \uc138\ud3ec \ubc16\uc73c\ub85c \ubc30\ucd9c\ub418\uc9c0 \uc54a\uc73c\ub098, \uc138\ud3ec\ub9c9\uc774 \uc190\uc0c1\ub418\uac70\ub098 \uc138\ud3ec\uac00 \uc8fd\ub294 \uacbd\uc6b0 \ubc30\uc9c0 \uc911\uc73c\ub85c \ubc29\ucd9c\ub429\ub2c8\ub2e4. \uadf8\ub807\uae30 \ub54c\ubb38\uc5d0 \ubc30\uc9c0\uc911\uc758 LDH\uc591\uc740 \uc8fd\uac70\ub098 \ud639\uc740 \uc0c1\ud574\ub97c \uc785\uc740 \uc138\ud3ec\uc758 \uc218\uc640 \ube44\ub840 \ud558\uac8c \ub429\ub2c8\ub2e4.<\/span><\/p>\n

EZ-LDH cytotoxicity Assay kit\ub294 \uc774\ub7ec\ud55c LDH\uc758 \ud2b9\uc131\uc5d0 \uae30\ubc18\ud558\uc5ec, \uc138\ud3ec\uc5d0\uc11c \ubc29\ucd9c\ub41c LDH\uc758 \uc591\uc744 water soluble tetrazolium salt (WST)\ub97c \uc774\uc6a9\ud558\uc5ec \ud761\uad11\ub3c4(450nm)\ub97c \uce21\uc815\ud558\ub294 \ubc29\uc2dd\uc744 \ud1b5\ud574 cell cytotoxicity test\ub97c \uac04\ud3b8\ud558\uace0 \ube60\ub974\uac8c \uc218\ud589\ud560 \uc218 \uc788\ub294 \uc81c\ud488\uc785\ub2c8\ub2e4.<\/span><\/p>\n

 <\/p>\n

Product details<\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n
\n

Detection Method<\/strong><\/span><\/p>\n<\/th>\n

\n

Colorimetric – Absorbance at 450nm\u00a0 (420-480 nm)<\/span><\/p>\n<\/td>\n<\/tr>\n

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Sample type<\/strong><\/span><\/p>\n<\/th>\n

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Cell media<\/span><\/p>\n<\/td>\n<\/tr>\n

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Assay type<\/strong><\/span><\/p>\n<\/th>\n

\n

Quantitative<\/span><\/p>\n<\/td>\n<\/tr>\n

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Kit Components\u00a0<\/strong><\/span><\/p>\n

&<\/strong><\/span><\/p>\n

Storage Condition<\/strong><\/span><\/p>\n<\/th>\n

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    \n
  • WST Substrate Mix \u00a0: -20\u2103<\/span><\/li>\n
  • LDH Assay Buffer : 0~4\u2103<\/span><\/li>\n
  • Cell Lysis Solution : 0~4\u2103<\/span><\/li>\n
  • Stop Solution: 0~4\u2103<\/span><\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
\n

Advantages<\/strong><\/span><\/p>\n<\/th>\n

\n
    \n
  • Fast<\/strong>\u00a0: 1\uc2dc\uac04 \ub0b4\uc5d0 \uc2e4\ud5d8\uc744 \ub05d\ub0b4\uc2e4 \uc218 \uc788\uc2b5\ub2c8\ub2e4.<\/span><\/li>\n
  • Accuracy<\/strong>\u00a0: \u00a0\ud761\uad11\ub3c4\uc640 \uc0c1\ud574\ub97c \uc785\uc740 \uc138\ud3ec\uc758 \uc218\ub294 \uc9c1\uc120\uc801\uc778 \uc0c1\uad00\uad00\uacc4\ub97c \ub098\ud0c0\ub0c5\ub2c8\ub2e4.<\/span><\/li>\n
  • Safety<\/strong>\u00a0: \u00a0\ub3d9\uc704\uc6d0\uc18c\ub97c \uc0ac\uc6a9\ud558\uc9c0 \uc54a\uc2b5\ub2c8\ub2e4.<\/span>
    \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \uc2dc\uc57d \uc790\uccb4 \ub3c5\uc131\uc774; \uc5c6\uc5b4 \uc2e4\ud5d8 \ud6c4, DNA \u00a0or RNA extraction,\u00a0<\/span>
    \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 western blot, MTT \ub4f1 \ucd94\uac00 \uc2e4\ud5d8\uc774 \uac00\ub2a5\ud569\ub2c8\ub2e4.<\/span><\/li>\n
  • Sensitivity<\/strong>\u00a0: \u00a0INT\ubc29\uc2dd\ubcf4\ub2e4 \ub354 \uc815\ud655\ud55c \uce21\uc815\uc774 \uac00\ub2a5\ud569\ub2c8\ub2e4.<\/span>
    \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 – 10% FBS \uac00 \ud568\uc720\ub41c media \uc0ac\uc6a9 \uac00\ub2a5.<\/span><\/li>\n
  • Low background<\/strong>\u00a0: \uc6d0\ub8cc\uc758 purity\uc640 \ucd5c\uc801\uc758 buffer \uc870\uc131\uc73c\ub85c\u00a0<\/span>
    \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 background\ub97c \ucd5c\uc18c\ud654\ud558\uc600\uc2b5\ub2c8\ub2e4.<\/span><\/li>\n
  • Flexibility<\/strong>\u00a0: \u00a0Adherent cell\uacfc Suspension cell \ubaa8\ub450 \uc0ac\uc6a9 \uac00\ub2a5\ud569\ub2c8\ub2e4.<\/span><\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
\n

Application<\/strong><\/span><\/p>\n<\/th>\n

\n
    \n
  • Cytotoxicity assay<\/span><\/li>\n
  • Cell mediated cytotoxicity assay<\/span>
    NK activity : Natural Killer \u00a0cell\u00a0<\/span>
    CTL : \u00a0Cytotoxicity T-Lymphocytes cell\u00a0<\/span>
    CMC : Complement Mediated Cytotoxicity<\/span>
    ADCC : \u00a0Antibody Dependent Cellular Cytotoxicity\u00a0<\/span>
    LAK : \u00a0Lymphokine Activated Killer cell<\/span><\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

     <\/p>\n

     <\/p>\n

    Data<\/strong><\/span><\/p>\n

     <\/p>\n

    \"\"<\/span><\/p>\n

    Fig. Assay Principle<\/strong><\/span><\/p>\n

    \uc138\ud3ec\uc5d0\uc11c \ubc29\ucd9c\ub41c LDH\ub294 Lactate\ub97c \ud0c8\uc218\uc18c\ud654 \uc2dc\ucf1c Pyruvate\uacfc NADH\ub97c \uc0dd\uc131\ud569\ub2c8\ub2e4.<\/span><\/p>\n

    \uc774 NADH\uac00 diaphorase\uc758 \ucd09\ub9e4\uc5d0 \uc758\ud574 WST\ub97c \ud658\uc6d0 \uc2dc\ucf1c 450nm \ud30c\uc7a5\uc5d0\uc11c \ud761\uad11\uc744 \uac16\ub294 \uc801\uc0c9\uc758 formazan\uc744 \ud615\uc131\ud569\ub2c8\ub2e4.<\/span><\/p>\n

    \ub54c\ubb38\uc5d0 \ubc30\uc9c0\uc911\uc758 LDH\uac00 \uc99d\uac00\ud558\uba74 formazan\uc758 \uc0dd\uc131\uc774 \uc99d\uac00\ud558\uc5ec \ud761\uad11\ub3c4\uac00 \uc99d\uac00\ud558\uba70, \uc774\ub294 450nm\uc758 \ud761\uad11\ub3c4 \uc99d\ub300\ub85c \uce21\uc815\ud560 \uc218 \uc788\uc2b5\ub2c8\ub2e4.<\/span><\/p>\n

     <\/p>\n

     <\/p>\n

    \"\"<\/span><\/p>\n

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    HOME DoGen Cell Based Assay EZ-LDH   Cell cytotoxicity assay kit Catalog No. Assay DG-LDH500 500 tests DG-LDH1000 1000 tests Description   EZ-LDH \ub294 WST(water soluble tetrazolium salt)\ub97c \uc0ac\uc6a9\ud558\uc5ec \u00a0apoptosis \ud639\uc740 necrosis\ub4f1\uc5d0 \uc758\ud558\uc5ec \uc8fd\uac70\ub098 \uc190\uc0c1\ub41c \uc138\ud3ec\uc5d0\uc11c \ubc29\ucd9c\ub418\ub294 Lactate dehydrogenase(LDH)\uc758 \uc591\uc744 \uace0\uac10\ub3c4\ub85c \uce21\uc815\ud568\uc73c\ub85c\uc368 cytotoxicity\/cytolysis\ub97c \uac04\ub2e8\ud558\uace0 \uc815\ud655\ud558\uac8c \uce21\uc815\ud560 \uc218 \uc788\ub294 kit \uc785\ub2c8\ub2e4. Cytotoxicity (Cell death)\ub294 cell viability […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"_links":{"self":[{"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=\/wp\/v2\/pages\/13598"}],"collection":[{"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=13598"}],"version-history":[{"count":5,"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=\/wp\/v2\/pages\/13598\/revisions"}],"predecessor-version":[{"id":14016,"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=\/wp\/v2\/pages\/13598\/revisions\/14016"}],"wp:attachment":[{"href":"http:\/\/itsbio.co.kr\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=13598"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}