CRISPR Gene Deletion & miRNA/lncRNA Knockout Service
Go beyond indel mutations! abm offers a custom CRISPR Gene Deletion and miRNA/lncRNA Knockout Service for unprecedented control of your CRISPR deletion.
CRISPR can be used to carry out large genomic deletions by utilizing dual sgRNAs, which target Cas9 cleavage upstream and downstream of the locus of interest. These CRISPR gene deletions benefit from the presence of a homology-directed repair (HDR) template, which allows for the precise repair of DNA via the HDR pathway. This ability is particularly useful for performing CRISPR lncRNA knockout or CRISPR miRNA knockout, as simple indel mutations do not facilitate effective knockout of miRNA or lncRNA. Order a gene deletion or miRNA/lncRNA knockout, and you’ll receive an edited cell line ready for use in downstream experiments.
Applications:
- Delete precise genomic sequences in your gene of interest
- Delete non-coding RNA sequences including microRNA (miRNA) and long non-coding RNA (lncRNA)
“We are quite happy with the knockout cells that you made. This was a tricky knockout and hemizygous removal was much more prevalent than the double knockout, but you did it. In addition to your excellent genomic sequence characterization of both mutant LIF alleles, I have confirmed in our lab that the tumor cells have lost all mouse LIF production”
Dr. Robert Jackman, Boston University, Mouse LIF CRISPR Knock Out C26 Tumor Cell Line Generation and Screening Service
Additional Resources:
Service Details
| BASIC PACKAGE 1 (CAT. NO. C316) | SERVICE INCLUDES: | DELIVERABLES | LEAD TIME |
|---|---|---|---|
| Custom miRNA or lncRNA Knockout Service |
|
Genetically engineered cell line (up to 2 vials 1×106 cells/vial) and custom service report | 22‑25 weeks |
| BASIC PACKAGE 2 (CAT. NO. C317) | SERVICE INCLUDES: | DELIVERABLES | LEAD TIME |
|---|---|---|---|
| Custom Specific Genomic Sequence Knockout Service |
|
Genetically engineered cell line (up to 2 vials 1×106 cells/vial) and custom service report | 22‑25 weeks |
| ADD‑ON SERVICES | UNIT | CAT. NO. |
|---|---|---|
| Cell Line of Choice for Gene Editing# | 1 Cell Line | C141 |
| WT Control Cell Line Expressing Cas9 for Comparison (50% discount for academic customers if ordered with the basic package) | 1 Cell Line | C142 |
| Additional Clones | 1 Clone | C143 |
| Additional Vials of Delivered Cells^^^ | 1 Vial | C144 |
| Western Blot Validation Service (Up to 10 Clones Tested)## | 1 Service | C145 |
| Off-Target Analysis by Whole Genome Sequencing | Per Clone | C146 |
| Additional Rounds of Selection & Screening by Sanger Sequencing | 1 Screening | C147 |
| CRISPR-Cas9 Targeted Amplicon Sequencing | 1 Service | IA00100 |
| Cell Line Authentication: STR Profiling of 1 WT Parental Cell Line & 1 Knock-Out Cell Line Clone (50% discount if ordered with the basic package) |
1 Service | C287 |
*Choose from HEK293, HEK293T, A549, HeLa, MDCK, A375, HepG2, HT1080 or U87MG. These cell lines are available as Cas-9 expressing versions or parental versions. Cas9 expressing version will be used by default. If the parental version is preferred, please request prior to order placement.
**The above pricing is valid for a single gene knockout for diploid loci only.
#Customer is required to provide at least 2 million cells, 1L of propagation media/any special coated flasks if needed (if cells need DMEM or RPMI, abm will supply media). Also, the cell line must tolerate single cell cloning and display adequate transfection and transduction efficiency.
##Pre-validated antibody needs to be provided by customer with appropriate positive control (preferably with supporting data).
^All “add on” services need to be indicated prior to order placement as the project design will need to be finalized prior to start of the service.
^^ Final Lead time may vary depending on actual growth rate of cell line while expanding from single cell.
Additional Info
Workflow
Additional Information & MTA
Orders of this service are subjected to the completion of a signed Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at technical@abmgood.com. The end user acknowledges that the Materials provided under abm’s MTA does not grant a license for commercial use or imply any ownership rights or any intellectual property rights relating to the Materials.
Please note that if the gene to be knocked out may be essential to cell survival, it is up to the end-user to proceed with the services. abm is unable to guarantee cell survival in these cases and will only attempt to rescue the clones under these conditions. abm is not accountable for cell survival if rescuing the clones (instructions to be provided customers) is unsuccessful. If customer chooses to proceed, abm will provide a hemizygous pool as the default deliverable to compensate for any lethal effects (unless otherwise requested).
***A deposit is required to initiate all CRISPR Cell Line projects
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