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MegaFi™ Fidelity DNA Polymerase

CAT.NO UNIT
G896 500 rxn / 20ul      (200 µl)

Specifications

Description

1300X lower error rates, 3X more affordable!

MegaFi™ Fidelity DNA Polymerase sets new standards for sensitive, robust, and high-fidelity PCR performance. This strategically-engineered polymerase has exceptional sensitivity and can amplify even the most difficult templates (compared to the leading competitors). In addition, MegaFi™ has high fidelity and ultra-low error rates (over 1,000X less than Taq polymerase, representing the lowest error rate on the market, see website for more information), making it incredibly useful for a variety of PCR applications demanding high fidelity, including Next Generation Sequencing, molecular cloning, or diagnostics.

Product Features:

  • 1300X lower error rates than regular Taq
  • Amplifiies difficult templates
  • Effective in long-range PCR
  • 3X more cost-effective than competing brands

 

PRODUCT COMPONENT QUANTITY
MegaFi™ Fidelity DNA Polymerase  500 rxn/20 ul   (200 µl)
5X MegaFi™ Buffer 1 2.5 ml (2 x 1.25 ml)

1 Buffer contains 1.5 mm Mg2+

 

SKU G896
Applications
  • Ultra high-fidelity PCR
  • Next Generation Sequencing
Storage Condition Store at -20 °C.
Unit quantity 500 rxn  / 20ul
CHARACTERISTIC MegaFi™ Fidelity
Proofreading Yes
Fidelity (vs. Native Taq) 1300X
Specificity ●●●●
Extension Speed 20-30 sec/kb
Target Length 15-20 kb
Suitable for TA cloning No
Format Enzyme (G896)
MasterMix (G897)
Special Feature Fidelity and Sensitivity

 

dNTP Mix

 

제품 번호 품   명    및    규    격
G050 dNTP Set           4 x 250 µl (100 mM each)
G010 dNTP Mix           250 µl, 10 mM each
G128 dNTP Mix           500 µl, 10 mM each
G129 dNTP Mix           1.0 ml, 10 mM each

FAQs

References

 

  • Chung, G et al. “CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans” DNA Repair (Amst.) 10 (11):1174-1182 (2011). DOI: 10.1016/j.dnarep.2011.09.011PubMed: 21968058. Application: PCR.