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abm Cell Biology Stem Cells and iPSCs Media Kits for Stem Cells iPSC Freezing Media
iPSC Freezing Media
| CAT.NO | UNIT |
|---|---|
| TM023 | 100ml |
| Description | The iPSC Freezing Medium is suitable for the cryopreservation of extremely sensitive cell types such as hematopoietic stem cells, mesenchymal stem cells and iPSCs. ABM’s iPSC Freezing Medium is an animal protein-free defined cryopreservation medium and have been tested to show increased cell recovery rate compared to conventional DMSO freezing medium. |
|---|---|
| SKU | TM023 |
| Sterility | Sterility tested for endotoxin, bacteria, yeast and fungi. |
| Storage Condition | Store at -20°C. |
| Unit quantity | 100ml |
Supporting Protocol
MSDS
QC
Other
What is the protocol for freezing iPSCs?
1) 4-5 days after cell split, when iPSCs reach a confluency of 30-60%, aspirate out spend medium and add 1ml 0.5mM EDTA/PBS. Incubate cells at 37C for ~5min. 2) When colonies start to detach, gently harvest all the cells into a 5-ml or 15-ml or 50-ml tube by pippeting 1-2 times. Avoid multiple pippetting. Breaking down clumps into single cells may substantially decrease cell survival. May add iPSC medium to harvest all the cells. 3) Spin down at 200g for 2-3 minutes. 4) Carefully aspirate out supernatent and gently resuspend cells in 0.5ml iPSC medium. 5) Add 0.5ml freezing medium (TM023) and ROCK inhibitor. Mix well by flipping the vial and transfer the medium to a cryovial. 6) Finally transfer the vial to -80C freezer for short-term storage (days to weeks). After cells are frozen, you may then transfer the vial to liquid nitrogen tank for long-term storage (years).
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